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Viruses, Volume 16, Issue 10

October 2024 - 140 articles

Cover Story: Expansion microscopy, a technique that physically enlarges samples using a swellable hydrogel before imaging, has recently gained attention for its capabilities in high-resolution imaging of biological structures. However, its application in the field of virology remains limited. In this study, we evaluate the ultrastructure expansion microscopy (U-ExM) protocol, which achieves roughly four-fold sample expansion, to visualize various post-entry stages of the HIV-1 life cycle. Our findings demonstrate that U-ExM effectively preserves and expands samples across a diverse range of cellular targets, including both infected and uninfected cell-culture-adapted and primary cells. We therefore believe that U-ExM represents a significant advancement for studying virus–host interactions with enhanced spatial resolution. View this paper
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Articles (140)

  • Case Report
  • Open Access
2 Citations
2,806 Views
12 Pages

Inflammasome-Driven Fatal Acute-on-Chronic Liver Failure Triggered by Mild COVID-19

  • Vivian Chih-Wei Chen,
  • Craig Ryan Joseph,
  • Wharton O. Y. Chan,
  • Wan Rong Sia,
  • Qi Su,
  • Xin Xiu Sam,
  • Hemavathi Tamilarasan,
  • Yun Yan Mah,
  • Wei Lun Ng and
  • Joe Yeong
  • + 5 authors

21 October 2024

Inflammasome is linked to many inflammatory diseases, including COVID-19 and autoimmune liver diseases. While severe COVID-19 was reported to exacerbate liver failure, we report a fatal acute-on-chronic liver failure (ACLF) in a stable primary biliar...

  • Project Report
  • Open Access
3 Citations
1,800 Views
13 Pages

Benchmarking of an Intervention Aiming at the Micro-Elimination of Hepatitis C in Vulnerable Populations in Perpignan, France, to Inform Scale-Up and Elimination on the French Territory

  • Gordana Avramovic,
  • Laura O’Doherty,
  • Tina McHugh,
  • Andre Jean Remy,
  • Arnaud Happiette,
  • Hakim Bouchkira,
  • Philippe Murat,
  • Olivier Scemama,
  • Adrien Esclade and
  • Maria Isabel Farfan Camacho
  • + 2 authors

21 October 2024

Hepatitis C virus (HCV) is an important cause of chronic liver disease. Among at-risk populations, access to care is challenging. The French Ministry of Health has supported a seek-and-treat pilot intervention aiming at micro-elimination in Perpignan...

  • Article
  • Open Access
5 Citations
4,699 Views
17 Pages

Insights into Genetic and Antigenic Characteristics of Influenza A(H1N1)pdm09 Viruses Circulating in Sicily During the Surveillance Season 2023–2024: The Potential Effect on the Seasonal Vaccine Effectiveness

  • Fabio Tramuto,
  • Carmelo Massimo Maida,
  • Giulia Randazzo,
  • Adriana Previti,
  • Giuseppe Sferlazza,
  • Giorgio Graziano,
  • Claudio Costantino,
  • Walter Mazzucco and
  • Francesco Vitale

21 October 2024

After disruption in the influenza circulation due to the emergence of SARS-CoV-2, the intensity of seasonal outbreaks has returned to the pre-pandemic levels. This study aimed to evaluate the evolution and variability of whole-genome sequences of A(H...

  • Article
  • Open Access
2,559 Views
21 Pages

The HIV-1 vpr R77Q Mutant Induces Apoptosis, G2 Cell Cycle Arrest, and Lower Production of Pro-Inflammatory Cytokines in Human CD4+ T Cells

  • Antonio Solis-Leal,
  • Dalton C. Karlinsey,
  • Sidney T. Sithole,
  • Jack Brandon Lopez,
  • Amanda Carlson,
  • Vicente Planelles,
  • Brian D. Poole and
  • Bradford K. Berges

21 October 2024

Acquired immunodeficiency syndrome (AIDS) occurs when HIV depletes CD4+ helper T cells. Some patients develop AIDS slowly or not at all, and are termed long-term non-progressors (LTNP), and while mutations in the HIV-1 Viral Protein R (vpr) gene such...

  • Article
  • Open Access
1 Citations
5,126 Views
18 Pages

Leveraging Synthetic Virology for the Rapid Engineering of Vesicular Stomatitis Virus (VSV)

  • Chad M. Moles,
  • Rupsa Basu,
  • Peter Weijmarshausen,
  • Brenda Ho,
  • Manal Farhat,
  • Taylor Flaat and
  • Bruce F. Smith

21 October 2024

Vesicular stomatitis virus (VSV) is a prototype RNA virus that has been instrumental in advancing our understanding of viral molecular biology and has applications in vaccine development, cancer therapy, antiviral screening, and more. Current VSV gen...

  • Article
  • Open Access
2 Citations
1,970 Views
18 Pages

Reexamining the Mycovirome of Botrytis spp.

  • Hugo Muñoz-Suárez,
  • Ana Ruiz-Padilla,
  • Livia Donaire,
  • Ernesto Pérez Benito and
  • María A. Ayllón

21 October 2024

Botrytis species cause gray mold disease in more than 200 crops worldwide. To control this disease, chemical fungicides are usually applied. However, more sustainable control alternatives should be explored, such as the use of hypovirulent mycovirus-...

  • Article
  • Open Access
5 Citations
2,000 Views
12 Pages

Evaluation of a Commercial Rapid Molecular Point-of-Care Assay for Differential Diagnosis Between SARS-CoV-2 and Flu A/B Infections in a Pediatric Setting

  • Paolo Bottino,
  • Costanza Massarino,
  • Christian Leli,
  • Elisabetta Scomparin,
  • Cristina Bara,
  • Franca Gotta,
  • Elisa Cornaglia,
  • Enrico Felici,
  • Michela Gentile and
  • Sara Ranzan
  • + 6 authors

20 October 2024

Given the ongoing COVID-19 pandemic, there is a need to identify SARS-CoV-2 and to differentiate it from other respiratory viral infections, especially influenza A and B, in various critical settings. Since their introduction, the use of rapid antige...

  • Article
  • Open Access
1,657 Views
16 Pages

The Role of Quantitative PCR in Evaluating the Clinical Significance of Human Bocavirus Detection in Children

  • Maja Mijač,
  • Tomislav Meštrović,
  • Irena Ivković-Jureković,
  • Tatjana Tot,
  • Jasmina Vraneš and
  • Sunčanica Ljubin-Sternak

19 October 2024

Human bocavirus (HBoV) has emerged as a significant pathogen primarily associated with respiratory infections in children. This study aimed to evaluate the clinical relevance of HBoV infection by quantifying viral loads in nasopharyngeal swabs from h...

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Viruses - ISSN 1999-4915