The platinum(II) complexes
trans-[PtCl
2(L
n)
2]∙
xSolv
1–
13 (Solv = H
2O or CH
3OH), involving N6-benzyladenosine-based N-donor ligands, were synthesized; L
n stands for N6-(2-methoxybenzyl)adenosine (
L1, involved in complex
1), N6-(4-methoxy-benzyl)adenosine (
L2,
2), N6-(2-chlorobenzyl)adenosine (
L3,
3), N6-(4-chlorobenzyl)-adenosine (
L4,
4), N6-(2-hydroxybenzyl)adenosine (
L5,
5), N6-(3-hydroxybenzyl)-adenosine (
L6,
6), N6-(2-hydroxy-3-methoxybenzyl)adenosine (
L7,
7), N6-(4-fluoro-benzyl)adenosine (
L8,
8), N6-(4-methylbenzyl)adenosine (
L9,
9), 2-chloro-N6-(3-hydroxy-benzyl)adenosine (
L10,
10), 2-chloro-N6-(4-hydroxybenzyl)adenosine (
L11,
11), 2-chloro-N6-(2-hydroxy-3-methoxybenzyl)adenosine (
L12,
12) and 2-chloro-N6-(2-hydroxy-5-methylbenzyl)adenosine (
L13,
13). The compounds were characterized by elemental analysis, mass spectrometry, IR and multinuclear (
1H-,
13C-,
195Pt- and
15N-) and two-dimensional NMR spectroscopy, which proved the N7-coordination mode of the appropriate N6-benzyladenosine derivative and
trans-geometry of the title complexes. The complexes
1–
13 were found to be non-toxic
in vitro against two selected human cancer cell lines (HOS and MCF7; with IC
50 > 50.0 µM). However, they were found (by ESI-MS study) to be able to interact with the physiological levels of the sulfur-containing biogenic biomolecule L-methionine by a relatively simple 1:1 exchange mechanism (one L
n molecule was replaced by one L-methionine molecule), thus forming a mixed-nitrogen/sulfur-ligand dichlorido-platinum(II) coordination species.
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