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Special Issue "Application of Novel Method in Pharmaceutical Study"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Medicinal Chemistry".

Deadline for manuscript submissions: 30 November 2018

Special Issue Editors

Guest Editor
Prof. Dr. Lesheng Teng

Department of Biopharmaceutics, School of Life Sciences, Jilin University, No.2699, Qianjin Street, Changchun 130012, China
Website | E-Mail
Interests: novel drug delivery system, innovative technologies, microfluidic, targeted therapy, nanotechnology
Guest Editor
Dr. Zhaogang Yang

Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA
E-Mail
Interests: innovative nanotechnologies, exosome, gene therapy, nanochannel electroporation, nanoparticle drug delivery
Guest Editor
Prof. Dr. Jing Xie

Department of Biopharmaceutics, School of Life Sciences, Jilin University, No.2699, Qianjin Street, Changchun 130012, China
Website | E-Mail
Interests: lipid nanoparticles, polymeric nanoparticles, liposomes, nucleic acid, toxicology, anticancer drug

Special Issue Information

Dear Colleagues,

With the improvements in medical treatment levels, the prevention and treatment of diseases that are difficult to cure have made important progress. Currently, a wide variety of drug formulations and technologies have been explored in malignant tumors, cardio cerebral vascular disease, anti-infection, geriatric diseases, etc. However, a number of problems still need to be solved before their utilization can become routine. These issues include low solubility, poor penetration into cells, hepatic disposition, narrow therapeutic index, rapid uptake by normal tissues, low bioavailability and high costs. Additionally, therapeutic drugs may have unfavorable pharmacokinetic parameters, such as a fast clearance rate and short terminal elimination half-life. Moreover, the emergence of drug resistance involving multiple mechanisms is a major obstacle to successful application in clinics. Therefore, in order to solve these problems, various novel strategies must be applied to pharmaceutical research. These methods include: Nanotechnology for the delivery of drugs, active targeting delivery of drugs for diseases therapy, scientific application of polymer materials and molecular biological methods, and so on. 

The purpose of this Special Issue is to present a series of application of novel methods in pharmaceutical study. We are committed to acquiring high-tech drugs that are fixed-time, fixed-position, and quantitative release. We hope these novel methods can help us overcome all the obstacles above.

Prof. Dr. Lesheng Teng
Dr. Zhaogang Yang
Prof. Dr. Jing Xie
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (5 papers)

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Research

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Open AccessArticle Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia
Molecules 2018, 23(11), 2874; https://doi.org/10.3390/molecules23112874
Received: 14 September 2018 / Revised: 26 October 2018 / Accepted: 28 October 2018 / Published: 4 November 2018
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Abstract
Ischemic injuries and local hypoxia can result in osteocytes dysfunction and play a key role in the pathogenesis of avascular osteonecrosis. Conventional imaging techniques including magnetic resonance imaging (MRI) and computed tomography (CT) can reveal structural and functional changes within bony anatomy; however,
[...] Read more.
Ischemic injuries and local hypoxia can result in osteocytes dysfunction and play a key role in the pathogenesis of avascular osteonecrosis. Conventional imaging techniques including magnetic resonance imaging (MRI) and computed tomography (CT) can reveal structural and functional changes within bony anatomy; however, characterization of osteocyte behavioral dynamics in the setting of osteonecrosis at the single cell resolution is limited. Here, we demonstrate an optical approach to study real-time osteocyte functions in vivo. Using nicotinamide adenine dinucleotide (NADH) as a biomarker for metabolic dynamics in osteocytes, we showed that NADH level within osteocytes transiently increase significantly after local ischemia through non-invasive photo-induced thrombosis of afferent arterioles followed by a steady decline. Our study presents a non-invasive optical approach to study osteocyte behavior through the modulation of local environmental conditions. Thus it provides a powerful toolkit to study cellular processes involved in bone pathologies in vivo. Full article
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Open AccessArticle Optimization of Fermentation Condition for Echinacoside Yield Improvement with Penicillium sp. H1, an Endophytic Fungus Isolated from Ligustrum lucidum Ait Using Response Surface Methodology
Molecules 2018, 23(10), 2586; https://doi.org/10.3390/molecules23102586
Received: 20 September 2018 / Revised: 30 September 2018 / Accepted: 1 October 2018 / Published: 10 October 2018
PDF Full-text (2889 KB) | HTML Full-text | XML Full-text
Abstract
(1) Background: Application of echinacoside has become increasingly important for its significant biological activities. However, there are many disadvantages in existing synthesis methods such as contaminating the environment, harsh reaction conditions and so on. Therefore, it is urgent to invent a novel alternative
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(1) Background: Application of echinacoside has become increasingly important for its significant biological activities. However, there are many disadvantages in existing synthesis methods such as contaminating the environment, harsh reaction conditions and so on. Therefore, it is urgent to invent a novel alternative method that can increase the yield of echinacoside. (2) Methods: In this study, we isolated and purified an endophyte from the leaves of Ligustrum lucidum Ait. Then, we improved the yield of echinacoside by optimizing the fermentation condition with an endophytic fungus. Penicillium sp. H1 was isolated from Ligustrum lucidum Ait. In addition, response surface methodology was used to optimize the fermentation condition. (3) Results: The results indicate that the maximal yield of echinacoside (37.16 mg/L) was obtained when inoculation rate, temperature and days were 13.98%, 27.85 °C and 26.06 days, respectively. The yield of echinacoside was 150.47 times higher under the optimal conditions than under the control conditions. The results indicate that the yield of echinacoside could be improved with endophytic fermentation by optimizing the fermentation condition. We provide an alternative method for echinacoside production by endophytic fermentation in this paper. It may have a profound effect on the application of echinacoside. Full article
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Open AccessArticle Study on the Quality Evaluation of Compound Danshen Preparations Based on the xCELLigence Real-Time Cell-Based Assay and Pharmacodynamic Authentication
Molecules 2018, 23(9), 2090; https://doi.org/10.3390/molecules23092090
Received: 16 June 2018 / Revised: 18 August 2018 / Accepted: 19 August 2018 / Published: 21 August 2018
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Abstract
Objective: To perform a preliminary study on the quality evaluation of compound Danshen preparations based on the xCELLigence Real-Time Cell-based Assay (RTCA) system and make a pharmacodynamics verification. Methods: The compound Danshen was discussed as a methodological example, and the bioactivity
[...] Read more.
Objective: To perform a preliminary study on the quality evaluation of compound Danshen preparations based on the xCELLigence Real-Time Cell-based Assay (RTCA) system and make a pharmacodynamics verification. Methods: The compound Danshen was discussed as a methodological example, and the bioactivity of the compound Danshen preparations were evaluated by real-time cell electronic analysis technology. Meanwhile, an in vivo experiment on an acute blood stasis rat model was performed in order to verify this novel evaluation through the curative effect of dissipating blood stasis. Results: We determined the cell index (CI) and IC50 of the compound Danshen preparations and produced time/dose-dependent cell response profiles (TCRPs). The quality of the three kinds of compound Danshen preparations was evaluated through the RTCA data. The trend of CI and TCRPs reflected the effect of drugs on the cell (promoting or inhibiting), and it was verified that the results correlated with the biological activity of the drugs using a pharmacodynamics experiment. Conclusion: The RTCA system can be used to evaluate the quality of compound Danshen Preparations, and it can provide a new idea and new method for quantitatively characterizing the biological activity of traditional Chinese medicines (TCMs). Full article
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Review

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Open AccessReview Microfluidic-Based Single-Cell Study: Current Status and Future Perspective
Molecules 2018, 23(9), 2347; https://doi.org/10.3390/molecules23092347
Received: 11 August 2018 / Revised: 5 September 2018 / Accepted: 9 September 2018 / Published: 13 September 2018
PDF Full-text (3102 KB) | HTML Full-text | XML Full-text
Abstract
Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic
[...] Read more.
Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic devices have played an increasingly important role in the field of single-cell study owing to their advantages: high efficiency, easy operation, and low cost. In this review, the applications of polymer-based microfluidics on cell manipulation, cell treatment, and cell analysis at single-cell level are detailed summarized. Moreover, three mainly types of manufacturing methods, i.e., replication, photodefining, and soft lithography methods for polymer-based microfluidics are also discussed. Full article
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Open AccessReview Recent Progress in Metal-Based Nanoparticles Mediated Photodynamic Therapy
Molecules 2018, 23(7), 1704; https://doi.org/10.3390/molecules23071704
Received: 4 June 2018 / Revised: 29 June 2018 / Accepted: 6 July 2018 / Published: 12 July 2018
Cited by 2 | PDF Full-text (1992 KB) | HTML Full-text | XML Full-text
Abstract
Photodynamic therapy (PDT) is able to non-invasively treat and diagnose various cancers and nonmalignant diseases by combining light, oxygen, and photosensitizers (PSs). However, the application of PDT is hindered by poor water solubility and limited light-penetration depth of the currently available photosensitizers (PSs).
[...] Read more.
Photodynamic therapy (PDT) is able to non-invasively treat and diagnose various cancers and nonmalignant diseases by combining light, oxygen, and photosensitizers (PSs). However, the application of PDT is hindered by poor water solubility and limited light-penetration depth of the currently available photosensitizers (PSs). Water solubility of PSs is crucial for designing pharmaceutical formulation and administration routes. Wavelength of light source at visible range normally has therapeutic depth less than 1 mm. In this review, focus is on the recent research progress of metal-based nanoparticles being applied in PDT. The potential toxicity of these nanoscales and future directions are further discussed. Full article
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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