Molecular Studies of Microorganisms in Plant Growth and Utilization

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Molecular Microbiology and Immunology".

Deadline for manuscript submissions: 31 August 2025 | Viewed by 607

Special Issue Editors


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Guest Editor
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
Interests: forage production key technology and integrated demonstration; study on the synergistic effect of cellulose decomposing bacteria and heterogeneous/homogeneous lactic acid bacteria to improve the quality of corn silage

E-Mail Website
Guest Editor Assistant
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
Interests: lactic acid bacteria; condensed tannins; aerobic exposure; paper mulberry; proteolysis

E-Mail
Guest Editor Assistant
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
Interests: silage; condensed tannins; Pediococcus pentosaceus

Special Issue Information

Dear Colleagues,

Microorganisms are important in plants, not only for growth but also in terms of utilization. Recently, the impact of abiotic stress on plants is intensifying due to global warming. In real-life environments, plants often face internal pressure from multiple sources, and these combined stresses will become the main factor restricting crop production in the near future. In addition to plants’ own resistance mechanisms against environmental stress, microorganisms also play an important role in their response to stress, for example, plant growth-promoting rhizobacteria (PGPR) such as Pseudomonas spp. and Bacillus spp. in the rhizosphere and arbuscular mycorrhizal fungi. Focusing on the functional role of microorganisms in plants’ resistance to environmental stress can inspire new techniques for maintaining agricultural production under changing circumstances, and is expected to be the first port of call for developing stress-resistant plant breeding. At present, although it is known which microorganisms are involved in plant stress, there is still a gap in our understanding of their specific regulatory mechanisms. On the other hand, ensiling is well known to be key way to more effectively utilize crops as animal feed resources. Microorganisms such as lactic acid bacteria and yeast are desired and undesired microorganisms during ensiling, respectively. Identifying the function and mechanisms of these microorganisms could improve crop utilization in the animal industry.

This Special Issue aims to explore the molecular mechanisms by which microorganisms mediate plant responses to abiotic stresses (e.g., drought, salinity, and temperature extremes) and enhance crop utilization through processes like ensiling. As such, we welcome contributions related to the application of advanced microbiology related to plant responses to abiotic stresses or related to crop utilization, such as fermentation during ensiling. The topics of interest include, but are not limited to, the following:

  1. The microbial mitigation of abiotic stress:
  • The role of PGPR (e.g., Pseudomonas and Bacillus) and mycorrhizal fungi in stress alleviation.
  • The molecular pathways involved in nutrient uptake, phytohormone modulation, and stress signaling.
  1. Omics-driven insights:
  • Genomic, transcriptomic, proteomic, and metabolomic studies elucidating plant–microbe interactions.
  • Microbial consortia and synthetic communities for enhanced resilience.
  1. Engineering microbial solutions:
  • CRISPR-edited microbes and microbiome engineering for stress tolerance.
  • Metabolic engineering of lactic acid bacteria (LAB) to optimize ensiling.
  1. Ensiling and crop utilization:
  • The dynamics of desirable (LAB) vs. undesirable (yeast) microbes in silage fermentation.
  • Strategies to suppress pathogens and enhance silage quality through microbial management.

Dr. Chunhui Ma
Guest Editor

Dr. Fanfan Zhang
Dr. Rongzheng Huang
Guest Editor Assistants

Manuscript Submission Information

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Keywords

  • plant growth-promoting rhizobacteria
  • abiotic stress
  • crop utilization
  • ensiling

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Published Papers (2 papers)

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Research

14 pages, 1444 KiB  
Article
The Effects of Lactococcus garvieae and Pediococcus pentosaceus on the Characteristics and Microbial Community of Urtica cannabina Silage
by Yongcheng Chen, Shuangming Li, Yingchao Sun, Yuxin Chai, Shuan Jia, Chunhui Ma and Fanfan Zhang
Microorganisms 2025, 13(7), 1453; https://doi.org/10.3390/microorganisms13071453 - 23 Jun 2025
Viewed by 216
Abstract
The utilization of nettle (Urtica cannabina) as feed is restricted by its material properties (antibacterial activity and high buffering capacity). This study hypothesized that the use of lactic acid bacteria (LAB) attached to nettles can improve these problems. Lactococcus garvieae (LG), [...] Read more.
The utilization of nettle (Urtica cannabina) as feed is restricted by its material properties (antibacterial activity and high buffering capacity). This study hypothesized that the use of lactic acid bacteria (LAB) attached to nettles can improve these problems. Lactococcus garvieae (LG), Pediococcus pentosaceus (PP), and LG + PP (LP) isolated from nettles were inoculated into nettle silage to explore nutrient retention and the microbial community structure. The results showed that inoculation significantly delayed dry matter and crude protein loss, inhibited neutral detergent fiber and acid detergent fiber degradation, and reduced ammonia nitrogen (NH3-N) accumulation. There was a significant increase in Firmicutes abundance after inoculation, and the dominant genus, Aerococcus, was negatively correlated with NH3-N accumulation. In the later stages of the PP treatment, Atopistipes synergistically inhibited Clostridia with acetic acid. However, the high buffering capacity and antibacterial components of raw nettle led to increased pH values during the later fermentation stages, limiting sustained acid production by LAB. These results confirm that nettle-derived LAB can effectively improve the quality of silage by regulating the microbial community and the acidification process; however, they must be combined with pretreatment strategies or optimized composite microbial agents to overcome raw material limitations. This study provides a theoretical basis and technical support for the utilization of nettle as feed. Full article
(This article belongs to the Special Issue Molecular Studies of Microorganisms in Plant Growth and Utilization)
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15 pages, 1837 KiB  
Article
Effect of Inoculation with Lactiplantibacillus plantarum and Cellulase on the Quality of Mulberry Silage
by Yingchao Sun, Yongcheng Chen, Zhiwei Huo, Guohong Liu, Xiaokai Zheng, Yayin Qi, Chunhui Ma and Fanfan Zhang
Microorganisms 2025, 13(7), 1437; https://doi.org/10.3390/microorganisms13071437 - 20 Jun 2025
Viewed by 248
Abstract
Lactic acid bacteria (LAB) and cellulase have been used as additives to improve the fermentation quality of mulberry silage. This study investigated the dynamics of fermentation characteristics and bacterial communities during 60-day ensiling through three established treatment groups: Control (no inoculation), Lactiplantibacillus plantarum [...] Read more.
Lactic acid bacteria (LAB) and cellulase have been used as additives to improve the fermentation quality of mulberry silage. This study investigated the dynamics of fermentation characteristics and bacterial communities during 60-day ensiling through three established treatment groups: Control (no inoculation), Lactiplantibacillus plantarum (LP) inoculation as well as combination of L. plantarum and cellulase inoculation group (LPC). The results showed that compared with the Control group, the LP and LPC treatments significantly reduced the loss of dry matter, soluble carbohydrates, and crude protein (p < 0.05), effectively promoted the accumulation of lactic acid and acetic acid (p < 0.05), but significantly elevated ammonia nitrogen (NH3-N) production. Inoculation was beneficial to the stability of the bacterial community in mulberry branch and leaf silage because it can maintain a high level of beneficial bacteria (Lactiplantibacillus) and inhibit the growth of harmful bacteria (Escherichia-Shigella). The combination of the inoculation of L. plantarum and cellulase may improve the quality of mulberry branch silage. Full article
(This article belongs to the Special Issue Molecular Studies of Microorganisms in Plant Growth and Utilization)
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