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Horticulturae
Special Issues
Tissue Culture Techniques and Molecular Markers of Horticultural Plants
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Tissue Culture Techniques and Molecular Markers of Horticultural Plants

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Propagation and Seeds".

Deadline for manuscript submissions: 20 August 2025 | Viewed by 3294

Special Issue Editors

Dr. Rytis Rugienius

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Guest Editor
Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, 54333 Babtai, Lithuania
Interests: horticultural plant breeding; biotechnology; physiology; stress resistance; cryopreservation
Special Issues, Collections and Topics in MDPI journals
Dr. Audrius Sasnauskas

E-Mail Website
Guest Editor
Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, 54333 Babtai, Lithuania
Interests: breeding of horticultural plants; genetic and genetic resources; variety testing
Dr. Akvile Virsile

E-Mail Website
Guest Editor
Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Kauno Str. 30, 54333 Babtai, Lithuania
Interests: plant photobiology; phytochemistry; metabolism; morphogenesis; ecophysiology

Special Issue Information

Dear Colleagues,

Micropropagation or in vitro technologies and marker-assisted selection are classic areas of biotechnology that have proven their worth over the past half century and continue to advance today. Different directions of in vitro plant cultivation research are developing, for example, screening in vitro, endophytic microorganisms, biopharming, gene editing and cryopreservation. There are very few in vitro techniques that work well for all genotypes. Often, other, more advanced technologies need to be found for specific recalcitrant genotypes. Here, advances in genomics, transcriptomics, proteomics and metabolomics provide significant new opportunities. This demonstrates the interrelationships of different plant systems under various stresses and physiological changes and makes it possible to find new high-throughput genetic markers related to valuable traits. A breakthrough in the use and application of molecular markers for breeding and fundamental research can only be expected when the markers cover the whole genome and different genetic systems in a wide and comprehensive manner.

We are pleased to invite you to submit papers for this Special Issue "Tissue Culture Techniques and Molecular Markers of Horticultural Plants", which aims to present the latest techniques in plant tissue culture and achievements of molecular markers research and applications.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: genetics, genomics, breeding and biotechnology, physiology, biochemistry and molecular biology, horticulture plant propagation, preservation of genetic resources, stress response, effect of stress to nutritional and phytochemical composition.

Dr. Rytis Rugienius
Dr. Audrius Sasnauskas
Dr. Akvile Virsile
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biotechnology
  • breeding
  • biopharming
  • controlled conditions
  • genetics
  • genetic resources
  • in vitro screening
  • micropropagation
  • phytochemical composition
  • stress resistancea

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

12 pages, 3026 KiB  
Article
Salt Stress-Induced Ascorbic Acid Accumulation and Its Trade-Off with Mannan Content in Tomato
by Chiaki Hasegawa, Kaori Yamada, Natsuki Hoyano, Mao Sano, Kiei Soyama and Hiroaki Iwai
Horticulturae 2025, 11(4), 400; https://doi.org/10.3390/horticulturae11040400 - 9 Apr 2025
Viewed by 366
Abstract
Salt stress causes osmotic stress and ion toxicity, often inhibiting plant growth and metabolism. However, salt-stressed tomato plants accumulate ascorbic acid, resulting in fruits with high commercial value. However, it was not well understood how mannose, the material for the synthesis of ascorbic [...] Read more.
Salt stress causes osmotic stress and ion toxicity, often inhibiting plant growth and metabolism. However, salt-stressed tomato plants accumulate ascorbic acid, resulting in fruits with high commercial value. However, it was not well understood how mannose, the material for the synthesis of ascorbic acid, and its metabolism are affected under salt stress conditions. In this study, we found that tomatoes grown under salinity stress had increased levels of ascorbic acid, which correlated with decreased levels of mannan in the skin and seeds. Expression analysis of the ascorbic acid synthase gene showed increased expression in early ripening stages under salt stress. In addition, the expression of cellulose synthase-like A (CSLA), genes involved in mannan metabolism, increased significantly during mid-ripening in the control condition. Since ascorbic acid and mannan share mannose as a precursor, they are likely to compete for it. This suggests that salt-stressed tomatoes may be deficient in both ascorbic acid and mannose, thereby affecting mannan synthesis. To investigate this trade-off, we developed a culture system with added mannose. The results showed that in salt-stressed tomatoes supplemented with mannose, ascorbic acid levels in unripe green peels reached those of fully ripe fruit, highlighting the influence of mannose availability on ascorbic acid accumulation. Full article
(This article belongs to the Special Issue Tissue Culture Techniques and Molecular Markers of Horticultural Plants)
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27 pages, 9691 KiB  
Article
Establishment of a Protocol for the Characterization of Secreted Biomolecules in Somatic Embryogenic Cultures of Olea europaea L.
by Rita Pires, Lénia Rodrigues, Fátima Milhano Santos, Iola F. Duarte, Sergio Ciordia, Augusto Peixe and Hélia Cardoso
Horticulturae 2025, 11(3), 331; https://doi.org/10.3390/horticulturae11030331 - 19 Mar 2025
Viewed by 399
Abstract
Somatic embryogenesis (SE) involves the formation of embryo-like structures from somatic cells without fertilization and is widely used for clonal propagation and genetic transformation. However, in olive (Olea europaea sp. europaea), SE remains challenging due to the recalcitrant behavior of adult [...] Read more.
Somatic embryogenesis (SE) involves the formation of embryo-like structures from somatic cells without fertilization and is widely used for clonal propagation and genetic transformation. However, in olive (Olea europaea sp. europaea), SE remains challenging due to the recalcitrant behavior of adult tissues when used as initial explants. Bioactive molecules released into the culture medium (conditioned medium, CM) by embryogenic cultures have been identified as modulators of the SE response. However, their potential role in enhancing SE efficiency in olive and overcoming tissue recalcitrance remains largely unexplored. To investigate the role of these biomolecules in olive SE, a protocol was established using SE cultures of cv. ‘Galega Vulgar’. Proteins and metabolites were separated by filtration, concentrated through lyophilization, and precipitated using three methods: Acetone, TCA/Acetone, and Methanol/Chloroform. The efficiency of these methods was evaluated through total protein quantification and via SDS-PAGE electrophoresis. LC-MS/MS was employed to analyze secretome composition using the TCA/Acetone precipitation method. Additionally, metabolite profiles were analyzed using 1H NMR spectroscopy. The results led to the identification of 1096 (526 protein groups) Olea europaea proteins, including well-known SE biomarkers such as kinases and peroxidases. NMR spectroscopy identified several metabolites secreted into the medium or resulting from the metabolic activity of secreted enzymes, confirming the applicability of the procedure. Although extracting secreted biomolecules from the culture medium presents significant challenges, the protocol established in this study successfully enabled the isolation and identification of both proteins and metabolites, revealing a valuable workflow for future in-depth analyses of secreted biomolecules in olive SE. Full article
(This article belongs to the Special Issue Tissue Culture Techniques and Molecular Markers of Horticultural Plants)
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14 pages, 6866 KiB  
Article
Recovery of Novel Sequence Variants in Chemically Mutagenized Seed and Vegetatively Propagated Coffea arabica L.
by Bradley J. Till, José P. Jiménez-Madrigal, Alfredo Herrera-Estrella, Karina Atriztán-Hernández and Andrés Gatica-Arias
Horticulturae 2024, 10(10), 1077; https://doi.org/10.3390/horticulturae10101077 - 9 Oct 2024
Cited by 1 | Viewed by 1962
Abstract
The negative effects of climate change impact both farmers and consumers. This is exemplified in coffee, one of the most widely consumed beverages in the world. Yield loss in high-quality Coffea arabica L., due to the spread of coffee leaf rust (Hemileia [...] Read more.
The negative effects of climate change impact both farmers and consumers. This is exemplified in coffee, one of the most widely consumed beverages in the world. Yield loss in high-quality Coffea arabica L., due to the spread of coffee leaf rust (Hemileia vastatrix), results in lower income for subsistence farmers and volatile prices in markets and cafes. Genetic improvement of crops is a proven approach to support sustainable production while mitigating the effects of biotic and abiotic stresses and simultaneously maintaining or improving quality. However, the improvement of many species, including coffee, is hindered by low genetic diversity. This can be overcome by inducing novel genetic variation via treatment of seeds or cells with mutagens. To evaluate this approach in coffee, mutant populations created by incubating seed or embryogenic calli with the chemical mutagens ethyl methanesulphonate or sodium azide were subject to reduced-representation DNA sequencing using the ddRADseq approach. More than 10,000 novel variants were recovered. Functional analysis revealed hundreds of sequence changes predicted to be deleterious for gene function. We discuss the challenges of unambiguously assigning these variants as being caused by the mutagenic treatment and describe purpose-built computational tools to facilitate the recovery of novel genetic variation from mutant plant populations. Full article
(This article belongs to the Special Issue Tissue Culture Techniques and Molecular Markers of Horticultural Plants)
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