The Functional Anatomy of Tree Xylem: From Cell Structure to Ecological Roles

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Forest Ecophysiology and Biology".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 1289

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Department of Forest Botany, Institute of Forest Sciences, Warsaw University of Life Sciences (WULS), Warsaw, Poland
Interests: wood biology; cambial events; wood formation; wood anatomy and function; wood ecology; wood hydraulic conductivity; wood structure modification; reaction wood; wood senescence; "wood" in monocotyledonous trees
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Dear Colleagues,

The secondary xylem (wood) formation is a complex process involving cambial cell divisions, enlargement of cambial cell derivatives, secondary cell wall deposition, and lignification. The autophagy of the cytoplasmic contents of the xylem cells marks the end of this process. In trees, wood formation results from the cross-talk between phytohormones and environmental signals.

The history of environmental events is encoded in the structure of secondary xylem, which is relatively simple in conifers and more complex in dicotyledonous trees. Analysis of the functional anatomical traits of secondary xylem provides insights into the environmental conditions during tree growth and the stresses experienced. The existing match between structure–function relationships and environmental requirements thus makes the secondary xylem of trees, as long-lived organisms, a key tissue in ecological research, especially in the era of climate change, because in addition to its leading physiological roles, i.e., water transport and mechanical support, it also serves as a carbon store. Therefore, we aim to invite contributions that use wood anatomy to study forest dynamics under climate change, fire history, or insect outbreaks. We assume key information on the phenotypic plasticity of trees functioning in different environmental conditions will be provided based on research on functional anatomical traits of secondary xylem.

Prof. Dr. Mirela Tulik
Guest Editor

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Keywords

  • autophagy
  • cell wall
  • conductive elements
  • dendroecology
  • heartwood
  • hormonal and environmental regulation
  • sapwood
  • xylem sap
  • xylem traits
  • trees' xylogenesis

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Published Papers (1 paper)

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Review

23 pages, 4270 KB  
Review
X-Ray Computed Microtomography and Investigations of Wood Structure and the Vascular Cambium
by David A. Collings and Ichirou Karahara
Forests 2026, 17(2), 286; https://doi.org/10.3390/f17020286 - 23 Feb 2026
Viewed by 792
Abstract
X-ray computed microtomography (µCT) provides an important complement to optical imaging for understanding the three-dimensional (3D) organization and function of xylem and wood. Unlike conventional sectioning, µCT is a non-destructive process that produces high-quality data sets that can be rotated, resliced and, following [...] Read more.
X-ray computed microtomography (µCT) provides an important complement to optical imaging for understanding the three-dimensional (3D) organization and function of xylem and wood. Unlike conventional sectioning, µCT is a non-destructive process that produces high-quality data sets that can be rotated, resliced and, following image segmentation, quantified. We highlight examples in which quantitative processing of 3D µCT sets has provided quantitative understanding of xylem and wood including the development and refilling of xylem embolisms, tree ring analyses and the development of interlocked grain. We also highlight two ways through which the µCT imaging of wood, and plants in general, will be improved. While the current staining protocols for plants are non-specific, developments in specific labeling techniques, including modifications of traditional electron microscopy stains for cell walls and recent developments in µCT imaging in non-plant specimens for studying antibody labeling and transgenes, should allow significant improvements in the imaging of xylem and wood by µCT. We also highlight machine learning which is already facilitating improvements in image segmentation and quantification of µCT data sets. When coupled with the recent advances in molecular genetics of the vascular cambium, these improvements in µCT should dramatically increase our understanding of xylem formation. Full article
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