Protein Hydrolysates: Structure, Functions and Their Application in Food

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Nutraceuticals, Functional Foods, and Novel Foods".

Deadline for manuscript submissions: 15 August 2025 | Viewed by 613

Special Issue Editors


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Guest Editor
Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. CIATEJ, Unidad Zapopan, Camino Arenero 1227, El Bajío, Zapopan 45019, Jalisco, Mexico
Interests: bioactive peptides; novel food ingredients; alternative proteins
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
1. Institute for Obesity Research, Tecnologico de Monterrey, Monterrey 64849, Mexico
2. Department of Bioengineering, School of Engineering & Sciences, Tecnologico de Monterrey, Puebla 72453, Mexico
Interests: plant based proteins; bioactive peptides; natural pigments; anthocyanins; obesity
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A. C., Avenida Normalistas 800, Colinas de la Normal, Guadalajara 44270, Jalisco, Mexico
Interests: plant biotechnology and biochemistry; functional foods; proteins of food interest: obtaining and characterization; fructans: obtaining; characterization and health effects

Special Issue Information

Dear Colleagues,

Dietary proteins play an essential role in multiple human metabolic functions. In addition to their physiological functions, proteins also participate in numerous physicochemical interactions within the food matrix when used as ingredients, resulting in different techno-functional properties. The solubility, emulsifying capacity, water retention capacity, oil retention capacity, foaming capacity, and gelling capacity of proteins are affected by protein structure, amino acid profile, and protein size, among other factors. To enhance consumers’ acceptance, the structure of proteins is often modified to improve proteins’ techno-functional properties and behavior in food formulations. Enzymatic protein hydrolysis has been widely used to modify proteins and generate protein hydrolysates and peptides. Protein hydrolysates have been recognized for their biological potential; moreover, those protein fractions could be added to food formulations as functional ingredients to improve their nutritional content or to improve the techno-functional properties of food. However, there is still a need to address important aspects of the use of protein hydrolysates as food ingredients, including the interaction with the food matrix, impact on food techno-functional properties, stability, flavor, and their impact on food acceptance.

This Special Issue invites the submission of original research articles and reviews that elucidate recent research on protein hydrolysates from different sources that can be used as food ingredients. Topics of interest include, but are not limited to, plant-based protein hydrolysates, alternative protein hydrolysates, peptide characterization, techno-functional properties of protein hydrolysates, food formulation using protein hydrolysates, and physicochemical properties of foods with added protein hydrolysates. A collection of high-quality manuscripts on these topics will be published in this Special Issue of Foods.

Dr. Luis Mojica
Dr. Diego A. Luna-Vital
Dr. Judith Esmeralda Urías-Silvas
Guest Editors

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Keywords

  • food proteins
  • peptides
  • protein hydrolysates
  • food ingredients
  • food formulation
  • physicochemical interactions
  • techno-functional properties

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Published Papers (1 paper)

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Research

12 pages, 708 KiB  
Article
Techno-Functional and Nutraceutical Assessment of Unprocessed and Germinated Amaranth Flours and Hydrolysates: Impact of the Reduction of Hydrolysis Time
by Alvaro Montoya-Rodríguez, Maribel Domínguez-Rodríguez, Eslim Sugey Sandoval-Sicairos, Evelia Maria Milán-Noris, Jorge Milán-Carrillo and Ada Keila Milán-Noris
Foods 2025, 14(15), 2666; https://doi.org/10.3390/foods14152666 - 29 Jul 2025
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Abstract
Amaranth is a nutritional and naturally gluten-free pseudocereal with several food applications. The germination and pepsin/pancreatin hydrolysis in amaranth releases antioxidant and anti-inflammatory compounds but the hydrolysis times (270 or 360 min) are too long to scale up in the development of amaranth [...] Read more.
Amaranth is a nutritional and naturally gluten-free pseudocereal with several food applications. The germination and pepsin/pancreatin hydrolysis in amaranth releases antioxidant and anti-inflammatory compounds but the hydrolysis times (270 or 360 min) are too long to scale up in the development of amaranth functional ingredients. The aim of this study was to estimate the influence of the germination and pepsin/pancreatin hydrolysis reduction time on the techno-functional properties and nutraceutical potential of amaranth flours and hydrolysates. The germination process increased 12.5% soluble protein (SP), 23.7% total phenolics (TPC), 259% water solubility, and 26% oil absorption in germinated amaranth flours (GAFs) compared to ungerminated amaranth flours (UAFs). The ungerminated (UAFH) and germinated (GAFH) amaranth hydrolysates showed values of degree of hydrolysis up to 50% with 150 min of sequential (pepsin + pancreatin) hydrolysis. The enzymatic hydrolysis released 1.5-fold SP and 14-fold TPC in both amaranth flours. The water solubility was higher in both hydrolysates than in their unhydrolyzed flour counterparts. The reduction in hydrolysis time did not significantly affect the nutraceutical potential of GAFH, enhancing its potential for further investigations. Finally, combining germination and enzymatic hydrolysis in amaranth enhances nutraceutical and techno-functional properties, increasing the seed. Consequently, GAF or GAFH could be used to elaborate on functional or gluten-free food products. Full article
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