Special Issue "Relevant Biomolecules for Germ Cells and Fertilization"

A special issue of Biomolecules (ISSN 2218-273X).

Deadline for manuscript submissions: 15 February 2020.

Special Issue Editors

Dr. Vanesa Robles
E-Mail Website
Guest Editor
Spanish Institute of Oceanography (IEO), Santander, 39012, Spain and MODCELL GROUP, Department of Molecular Biology, Universidad de León, 24071 León, Spain
Interests: reproductive and molecular biology; cryobiology; spermatology
Dr. Marta F. Riesco
E-Mail Website
Guest Editor
Spanish Institute of Oceanography (IEO), Santander, 39012, Spain
Interests: spermatology; germ cell specification and transplant; cryopreservation; molecular biomarkers; male reproduction
Dr. David G. Valcarce
E-Mail Website
Guest Editor
Spanish Institute of Oceanography (IEO), Santander, 39012, Spain
Interests: spermatology; probiotics; sperm quality; molecular biology

Special Issue Information

Dear Colleagues,

The field of reproduction has experimented important advances during the last years. The discovery of novel germ cell biomarkers, the potential of some bioactive substances in reproduction, and the development of new assays to evaluate the effect of techniques such as cryopreservation or PGC xenotransplantation on germ cell biomolecules, are only some of the fascinating advances achieved in the field. With this special topic-focused compilation on “Relevant biomolecules for germ cells and fertilization”, freely accessible online from all over the world, we would like to provide to a general audience with a large overview of the present state of our knowledge on this field. Our objective is to assemble a series of reviews or original articles in which everyone could find up-to-date information on the different issues directly connected to this topic.

We are thus welcoming any manuscript on 1) the role of germ cell proteins, coding, and non-coding RNAs as reproductive biomarkers, 2) the use of antioxidant or other bioactive molecules in reproduction, 3) the impact of biotechnologies on germ cells, and 4) the importance of crucial biomolecules for primordial germ cell specification and for gamete quality.

Dr. Vanesa Robles
Dr. Marta F. Riesco
Dr. David G. Valcarce
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • germ cells
  • gamete quality
  • cryopreservation
  • biomarkers
  • antioxidants

Published Papers (4 papers)

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Research

Open AccessArticle
Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (Sus scrofa) and Giant Panda (Ailuropoda melanoleuca)
Biomolecules 2019, 9(9), 432; https://doi.org/10.3390/biom9090432 - 01 Sep 2019
Cited by 1
Abstract
Post-thawed sperm quality parameters vary across different species after cryopreservation. To date, the molecular mechanism of sperm cryoinjury, freeze-tolerance and other influential factors are largely unknown. In this study, significantly dysregulated microRNAs (miRNAs) and mRNAs in boar and giant panda sperm with different [...] Read more.
Post-thawed sperm quality parameters vary across different species after cryopreservation. To date, the molecular mechanism of sperm cryoinjury, freeze-tolerance and other influential factors are largely unknown. In this study, significantly dysregulated microRNAs (miRNAs) and mRNAs in boar and giant panda sperm with different cryo-resistance capacity were evaluated. From the result of miRNA profile of fresh and frozen-thawed giant panda sperm, a total of 899 mature, novel miRNAs were identified, and 284 miRNAs were found to be significantly dysregulated (195 up-regulated and 89 down-regulated). Combined analysis of miRNA profiling of giant panda sperm and our previously published data on boar sperm, 46, 21 and 4 differentially expressed (DE) mRNAs in boar sperm were believed to be related to apoptosis, glycolysis and oxidative phosphorylation, respectively. Meanwhile, 87, 17 and 7 DE mRNAs in giant panda were associated with apoptosis, glycolysis and oxidative phosphorylation, respectively. Gene ontology (GO) analysis of the targets of DE miRNAs showed that they were mainly distributed on membrane related pathway in giant panda sperm, while cell components and cell processes were tied to the targets of DE miRNAs in boar sperm. Finally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DE mRNAs indicated that most of these DE mRNAs were distributed in membrane signal transduction-related pathways in giant panda sperm, while those in boar sperm were mainly distributed in the cytokine-cytokine receptor interaction pathway and inflammatory related pathways. In conclusion, although the different freezing extenders and programs were used, the DE miRNAs and mRNAs involved in apoptosis, energy metabolism, olfactory transduction pathway, inflammatory response and cytokine-cytokine interactions, could be the possible molecular mechanism of sperm cryoinjury and freeze tolerance. Full article
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
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Open AccessArticle
Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model
Biomolecules 2019, 9(8), 338; https://doi.org/10.3390/biom9080338 - 03 Aug 2019
Abstract
The aim of the present experiment is to study the effects of oral ingestion of a mixture of two probiotic bacteria on sperm quality and progenies. Three homogeneous groups of juvenile zebrafish were created. Once having reached adulthood (3 months postfertilization; mpf), each [...] Read more.
The aim of the present experiment is to study the effects of oral ingestion of a mixture of two probiotic bacteria on sperm quality and progenies. Three homogeneous groups of juvenile zebrafish were created. Once having reached adulthood (3 months postfertilization; mpf), each group received different feeding regimens: a standard diet (control), a maltodextrin-supplemented diet (vehicle control), or a probiotic-supplemented diet (a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347). The feeding regime lasted 4.5 months. Growth parameters (weight and length) were determined at 3, 5, and 7.5 mpf. Sperm motility was evaluated using computer-assisted sperm analysis at 5 and 7.5 mpf. Progeny survival, hatching rate, and malformation rate were also evaluated. Results showed that probiotic-supplemented diet improved growth parameters compared with the standard diet. The highest percentage of motile spermatozoa was reported in the probiotic-fed group. Concomitantly, the percentage of fast sperm subpopulation was significantly lower in samples derived from control males. Furthermore, there was a significant difference in progeny survival between the probiotic-fed group and the control group at three developmental times (24 hours postfertilization (hpf), 5 days postfertilization (dpf) and 7 dpf). In conclusion, in zebrafish, prolonged ingestion of a mixture of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 has positive effects on growth, sperm quality, and progeny survival. Full article
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
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Open AccessCommunication
Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
Biomolecules 2019, 9(8), 333; https://doi.org/10.3390/biom9080333 - 01 Aug 2019
Abstract
Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our [...] Read more.
Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only. Full article
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
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Open AccessArticle
Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity
Biomolecules 2019, 9(8), 307; https://doi.org/10.3390/biom9080307 - 25 Jul 2019
Abstract
A large amount of chemicals are released to the environment each year. Among them, bisphenol A (BPA) is of utmost concern since it interferes with the reproductive system of wild organisms due to its capacity to bind to hormone receptors. Additionally, BPA epigenotoxic [...] Read more.
A large amount of chemicals are released to the environment each year. Among them, bisphenol A (BPA) is of utmost concern since it interferes with the reproductive system of wild organisms due to its capacity to bind to hormone receptors. Additionally, BPA epigenotoxic activity is known to affect basic processes during embryonic life. However, its effects on primordial germ cells (PGCs) proliferation and migration, both mechanisms being crucial for gametogenesis, remain unknown. To investigate the effects of BPA on PGCs migration and eventual testicle development, zebrafish embryos were exposed to 100, 2000 and 4000 µg/L BPA during the first 24 h of development. Vasa immunostaining of PGCs revealed that exposure to 2000 and 4000 µg/L BPA impaired their migration to the genital ridge. Two pivotal genes of PGCs migration (cxcr4b and sdf1a) were highly dysregulated in embryos exposed to these doses, whereas DNA methylation and epigenetic marks in PGCs and their surrounding somatic cells were not altered. Once embryos reached adulthood, the morphometric study of their gonads revealed that, despite the reduced number of PGCs which colonized the genital ridges, normal testicles were developed. Although H3K9ac decreased in the sperm from treated fishes, it did not affect the progeny development. Full article
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

1. Authors: Oihane Diaz de Cerio, Lander Reina, Valeria Squatrito, Nestor Etxebarria, Belen Gonzalez-Gaya, Ibon Cancio

Title: "Ovothiol, a biomolecule to protect the oocytes of marine mussels from oxidative stress during spawning in polluted waters and from oxidative burst upon fertilization"

2. Authors: Changjun Zeng et al.

Title: "Comparative analysis of microRNA and mRNA profiles of sperm with different freeze tolerance capacity in boar and giant panda (Ailuropoda melanoleuca)"

3. Authors: Abdul Rasheed Baloch et al.

Title: "Labelling of Primordial Germ Cells in Sturgeon Using Iron Oxide Nanoparticles"

4. Authors: David G. Valcarce, Marta F. Riesco, Juan Manuel Martínez-Vázquez, Vanesa Robles

Title: "Long exposure to a diet supplemented with antioxidant and anti-inflammatory probiotics improves sperm quality and progeny survival in the zebrafish model"

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