New Insights into RNA-Binding Proteins

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Biomacromolecules: Proteins".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 1438

Special Issue Editor


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Guest Editor
MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UK
Interests: viral RNA processing; HIV-1; RNA binding proteins; virus-host interaction; antivirals; innate immunity
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Special Issue Information

Dear Colleagues,

RNA binding proteins are orchestrating every step in the life cycle of RNA from transcription to decay. With the advent of large-scale omics approaches, our understanding of the diversity of RNA binding proteins has dramatically increased. This allows us to investigate them in unprecedented depth, discovering not only new roles in various aspects of cell biology and disease, but also new regulatory functions and interplays. Biomolecules has taken this as an opportunity to acknowledge the importance and rapid development of the field in a Special Issue.

This Special Issue of Biomolecules seeks all types of manuscripts (e.g., reviews, original research articles, and short communications) on RNA binding proteins, including articles describing recent structural, biochemical, and cellular studies that have advanced our understanding of RNA binding proteins.

Dr. Marko Noerenberg
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • RNA-binding protein
  • RBP
  • RNA
  • riboregulation
  • RNA interactome
  • protein–RNA interaction
  • protein–RNA complex
  • ribonucleoprotein

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Published Papers (1 paper)

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Research

19 pages, 3012 KiB  
Article
RNA Surveillance Factor SMG5 Is Essential for Mouse Embryonic Stem Cell Differentiation
by Chengyan Chen, Yanling Wei, Xiaoning Jiang and Tangliang Li
Biomolecules 2024, 14(8), 1023; https://doi.org/10.3390/biom14081023 - 17 Aug 2024
Viewed by 1113
Abstract
Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling [...] Read more.
Nonsense-mediated mRNA decay (NMD) is a highly conserved post-transcriptional gene expression regulatory mechanism in eukaryotic cells. NMD eliminates aberrant mRNAs with premature termination codons to surveil transcriptome integrity. Furthermore, NMD fine-tunes gene expression by destabilizing RNAs with specific NMD features. Thus, by controlling the quality and quantity of the transcriptome, NMD plays a vital role in mammalian development, stress response, and tumorigenesis. Deficiencies of NMD factors result in early embryonic lethality, while the underlying mechanisms are poorly understood. SMG5 is a key NMD factor. In this study, we generated an Smg5 conditional knockout mouse model and found that Smg5-null results in early embryonic lethality before E13.5. Furthermore, we produced multiple lines of Smg5 knockout mouse embryonic stem cells (mESCs) and found that the deletion of Smg5 in mESCs does not compromise cell viability. Smg5-null delays differentiation of mESCs. Mechanistically, our study reveals that the c-MYC protein, but not c-Myc mRNA, is upregulated in SMG5-deficient mESCs. The overproduction of c-MYC protein could be caused by enhanced protein synthesis upon SMG5 loss. Furthermore, SMG5-null results in dysregulation of alternative splicing on multiple stem cell differentiation regulators. Overall, our findings underscore the importance of SMG5-NMD in regulating mESC cell-state transition. Full article
(This article belongs to the Special Issue New Insights into RNA-Binding Proteins)
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