Recent Advances in Molecular Genetics of Bacteria

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Molecular Genetics".

Deadline for manuscript submissions: 31 October 2025 | Viewed by 704

Special Issue Editor


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Guest Editor
Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
Interests: prokaryotic genome design and synthesis; genome rearrangement and evolution

Special Issue Information

Dear Colleagues,

The field of bacterial molecular genetics has witnessed significant advances in recent years, shedding light on the mechanisms that govern bacterial adaptation, survival, and pathogenicity. This Special Issue aims to highlight recent breakthroughs on the molecular genetics of bacteria, including the regulation of gene expression, bacterial evolution, and the interactions between bacterial genomes and the environment. The focus will be on studies that explore the genetic basis of bacterial behavior, resistance mechanisms, and the impact of genetic modifications on bacterial virulence and fitness.

Despite the rapid progress in this field, many questions remain unanswered, particularly in the areas of horizontal gene transfer, the molecular basis of bacterial resistance, and the role of genetic diversity in shaping bacterial populations.

This Special Issue will cover a broad range of topics, including but not limited to:

  • Bacterial gene regulation;
  • CRISPR-Cas systems;
  • Genome-wide association studies;
  • Microbial genomics;
  • The genetic underpinnings of bacterial pathogenesis.

We request original articles, brief communications, and review articles that will provide insights into the latest research in bacterial molecular genetics.

Dr. Bin Jia
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacterial gene regulation
  • CRISPR-Cas systems
  • bacterial resistance
  • microbial genomics
  • genome-wide association studies
  • bacterial pathogenesis
  • genetic diversity

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Published Papers (1 paper)

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Research

16 pages, 4265 KiB  
Article
Absolute Quantitative Metagenomic Analysis Provides More Accurate Insights for the Anti-Colitis Effect of Berberine via Modulation of Gut Microbiota
by Jiaguo Zhan, Jiale Cheng, Wenhui Chang, Yuying Su, Xixin Yue and Chongming Wu
Biomolecules 2025, 15(3), 400; https://doi.org/10.3390/biom15030400 - 11 Mar 2025
Viewed by 573
Abstract
Current gut microbiota studies often rely on relative quantitative sequencing. However, under certain circumstances, while the relative quantitative abundance of these bacteria may remain stable, the absolute quantities of specific bacteria can vary considerably. Since the function of bacteria is directly linked to [...] Read more.
Current gut microbiota studies often rely on relative quantitative sequencing. However, under certain circumstances, while the relative quantitative abundance of these bacteria may remain stable, the absolute quantities of specific bacteria can vary considerably. Since the function of bacteria is directly linked to their total numbers, absolute quantification is crucial. This study aims to identify the optimal method for microbiome analysis by comparing relative and absolute quantitative sequencing. Using ulcerative colitis, which is closely associated with gut microbiota, as a disease model and berberine (which affects microbiota) versus sodium butyrate (which does not) as drugs, relative and absolute quantitative methods were used to evaluate the varying effects of the different drugs on the regulation of gut microbiota in UC-affected animals. The regulatory effects of BBR on gut microbiota were further synthesized as identified in earlier studies using an individual-based meta-analysis, and we compared these findings with our absolute sequencing results. The results from absolute sequencing were more consistent with the actual microbial community, suggesting that relative abundance measurements might not accurately reflect the true abundance of microbial species. Moreover, meta-analysis results were only partially consistent with absolute quantitative sequencing and sometimes directly opposed, suggesting that relative quantitative sequencing analyses are prone to misinterpretation and incorrect correlation of results. This study underscores the importance of absolute quantitative analysis in accurately representing the true microbial counts in a sample and evaluating the modulatory effects of drugs on the microbiome, which plays a vital role in the study of the microbiome. Full article
(This article belongs to the Special Issue Recent Advances in Molecular Genetics of Bacteria)
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