Optimization and Innovation of Physiological Indexes in Crop Tolerance Mechanism

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Plant-Crop Biology and Biochemistry".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1370

Special Issue Editors


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Guest Editor
Department of Agriculture and Nutrition, Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, Croatia
Interests: chlorophyll fluorescence indexes; gas exchange indexes; gene expression analysis; abiotic stress (drought, heat stress, and heavy metal stress); plant physiology; biostimulants

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Guest Editor
Department of Agriculture and Nutrition, Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, Croatia
Interests: plant polyphenols; volatiles; biostimulants; protein hydrolysates; foliar fertilization; crop yield and quality
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Special Issue Information

Dear Colleagues,

Abiotic stress factors are a major factor in the limitation of crop yields. With the change in climate, stress factors such as temperature extremes, drought, salinity, cold, and flooding, among others, are becoming more frequent and causing severe decreases in crop production worldwide. Due to the globality of these stress factors, predictions of crop yield models show losses in major crop cultures, such as rice, maize, and wheat, which can lead to serious consequences for global food security. With the advancements in technology, various instruments, techniques, and methods have been, and still are, developed for the data collection of various morphological and physiological traits and processes in the agricultural sector. This sector is going through a digital revolution, as computers are now used basically in all agriculture-related processes, and data collection is not only reserved for research. With the integration of the Internet of Things (IoT), devices can also communicate with decision support software, which aids in field management.

With radical increases in the data points collected and the increasing number of devices and physiological parameters measured, it is important to search the data for predictive variables and reduce the data to form indexes that are sensitive to the physiological process that is of interest. In our view, some of the most important indexes are based on chlorophyll a fluorescence measurement, the ratio of variable and maximum fluorescence (Fv/Fm), and the performance index (PIABS), which are reduced under different kinds of abiotic stresses. Although they are very useful, they are not very specific to a certain kind of stress. Indexes such as Na+/K+ ratios and stomatal density have showed promising results as screening indexes for salt stress; Fv/Fm combined with stomatal density and residual transpiration have showed interesting results under drought stress and have the potential to be used by plant breeders; multidimensional evaluation has also been used to source the key contributing heat tolerance indexes in wheat. Genetic parameters such as abscisic acid, aquaporins, dehydrins, various transcription factors, heat shock proteins, antioxidant enzymes, small RNA molecules, and many others introduce a whole new field of indexes to be utilized and combined with other physiological parameters.

This Special Issue will focus on the “Optimization and innovation of physiological indexes in crop tolerance”. In this Special Issue, original research articles, review articles, communications, letters, and opinions are welcome, providing insights into all related topics of interest that include the use, optimization, and formulation of new innovative indexes for sensing the effects of stressful factors and assessing the severity of single or multiple types of stresses to which crops are exposed. New indexes and optimizations of already existing indexes could spark new research interests, aid in breeding programs, and hence reduce the impact of climate change.

Dr. Mario Franić
Dr. Igor Pasković
Guest Editors

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Keywords

  • abiotic stress
  • crop resilience
  • physiological traits
  • stress adaptation
  • agricultural innovation
  • tolerance mechanisms
  • yield stability
  • physiological index
  • performance index

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Published Papers (2 papers)

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Research

30 pages, 6093 KiB  
Article
Investigation of Antioxidative Enzymes and Transcriptomic Analysis in Response to Foliar Application of Zinc Oxide Nanoparticles and Salinity Stress in Solanum lycopersicum
by Mostafa Ahmed, Zoltán Tóth, Roquia Rizk, Donia Abdul-Hamid and Kincső Decsi
Agronomy 2025, 15(7), 1715; https://doi.org/10.3390/agronomy15071715 - 16 Jul 2025
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Abstract
Farmers commonly throw away tomato leaves when they harvest tomatoes, although they are a good source of vital biomolecules. ZnO nanoparticles (ZnO NPs) enhance plant growth by regulating abiotic stress and scavenging reactive oxygen species. In the current article, the activities of five [...] Read more.
Farmers commonly throw away tomato leaves when they harvest tomatoes, although they are a good source of vital biomolecules. ZnO nanoparticles (ZnO NPs) enhance plant growth by regulating abiotic stress and scavenging reactive oxygen species. In the current article, the activities of five antioxidant enzymes—glutathione reductase (GR), peroxidase (POX), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT)—were determined spectrophotometrically to study the interaction between foliar fertilization of ZnO NPs and salt stress in tomato plants. We employed the next-generation sequencing (NGS) technique to investigate the gene expression. It was also used to generate a de novo supertranscript and then determine the sequences modulated by treatments. Differential expression analysis was used to identify increased and reduced gene clusters, and gene enrichment analysis was used to identify over- and under-expressed genes under the treatment. Gene Ontology (GO) was used to identify the functions and regulatory pathways of the differentially expressed genes (DEGs). It was found that ZnO nanoparticles had the capability to overcome the reduction in antioxidant enzyme production levels in the case of the salinity-stressed treatments and enhance the secretion of those enzymes in the non-stressed but sprayed treatments. The ZnO NPs also enhanced the reduction in stress-responsive genes associated with salt stress resistance. The results revealed the impact of ZnO nanoparticles on alleviating the salinity stress reductive effects in antioxidative enzymes and regulating the mechanism by which metabolically relevant genes adaptively respond to salt stress in tomato plants. So, spraying tomato plants (stressed or not) with ZnO NPs is a promising agricultural technique in improving different metabolic pathways that are responsible for plants’ resistance. Full article
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16 pages, 1945 KiB  
Article
Debaryomyces hansenii Enhances Growth, Nutrient Uptake, and Yield in Rice Plants (Oryza sativa L.) Cultivated in Calcareous Soil
by Jorge Núñez-Cano, Francisco J. Ruiz-Castilla, José Ramos, Francisco J. Romera and Carlos Lucena
Agronomy 2025, 15(7), 1696; https://doi.org/10.3390/agronomy15071696 - 14 Jul 2025
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Abstract
Calcareous soils, characterized by high pH and calcium carbonate content, often limit the availability of essential nutrients for crops such as rice (Oryza sativa L.), reducing yield and nutritional quality. In this study, we evaluated the effect of the halotolerant yeast Debaryomyces [...] Read more.
Calcareous soils, characterized by high pH and calcium carbonate content, often limit the availability of essential nutrients for crops such as rice (Oryza sativa L.), reducing yield and nutritional quality. In this study, we evaluated the effect of the halotolerant yeast Debaryomyces hansenii on the growth, nutrient uptake, and phosphorus acquisition mechanisms of rice plants cultivated in calcareous soil under controlled greenhouse conditions. Plants inoculated with D. hansenii, particularly via root immersion, exhibited significantly higher SPAD chlorophyll index, plant height, and grain yield compared to controls. A modest increase (~4%) in dry matter content was also observed under sterilized soil conditions. Foliar concentrations of Fe, Zn, and Mn significantly increased in plants inoculated with D. hansenii via root immersion in non-sterilized calcareous soil, indicating improved micronutrient acquisition under these specific conditions. Although leaf phosphorus levels were not significantly increased, D. hansenii stimulated acid phosphatase activity, as visually observed through BCIP staining, and upregulated genes involved in phosphorus acquisition under both P-sufficient and P-deficient conditions. At the molecular level, D. hansenii upregulated the expression of acid phosphatase genes (OsPAP3, OsPAP9) and a phosphate transporter gene (OsPTH1;6), confirming its influence on P-related physiological responses. These findings demonstrate that D. hansenii functions as a plant growth-promoting yeast (PGPY) and may serve as a promising biofertilizer for improving rice productivity and nutrient efficiency in calcareous soils, contributing to sustainable agricultural practices in calcareous soils and other nutrient-limiting environments. Full article
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