Next Issue
Volume 4, March
Previous Issue
Volume 3, September
 
 

Waste, Volume 3, Issue 4 (December 2025) – 13 articles

  • Issues are regarded as officially published after their release is announced to the table of contents alert mailing list.
  • You may sign up for e-mail alerts to receive table of contents of newly released issues.
  • PDF is the official format for papers published in both, html and pdf forms. To view the papers in pdf format, click on the "PDF Full-text" link, and use the free Adobe Reader to open them.
Order results
Result details
Select all
Export citation of selected articles as:
15 pages, 2395 KB  
Article
Sustainable Valorization of Juice Industry Wastes: A Life Cycle Assessment Case Study
by Fotini Drosou, Tryfon Kekes, Athanasios Kardamanidis and Magdalini Krokida
Waste 2025, 3(4), 42; https://doi.org/10.3390/waste3040042 - 18 Dec 2025
Abstract
The juice industry generates substantial quantities of solid waste and wastewater. Consequently, efforts have focused on their treatment and valorization to obtain high-value-added products. Traditionally, these wastes are managed through landfill disposal and treatment in municipal wastewater facilities, respectively. In the present work, [...] Read more.
The juice industry generates substantial quantities of solid waste and wastewater. Consequently, efforts have focused on their treatment and valorization to obtain high-value-added products. Traditionally, these wastes are managed through landfill disposal and treatment in municipal wastewater facilities, respectively. In the present work, two alternative scenarios for the valorization of orange juice waste were developed and assessed in comparison to the conventional approach by performing a Life Cycle Assessment (LCA). Scenario 1 involved hydro-distillation of solid waste for essential oil recovery, followed by anaerobic digestion for biogas and fertilizer production, with wastewater treated via membrane filtration and chlorination. In Scenario 2, solvent-free microwave extraction (SFME) was employed for essential oil recovery, followed by anaerobic digestion. Wastewater was treated in a membrane bioreactor followed by ultraviolet treatment. According to the results, Scenario 1 achieved a 36% reduction in greenhouse gas emissions due to the beneficial effects of biogas and fertilizer production, despite its high energy demands. Scenario 2 exhibited the best environmental performance due to lower energy demands and higher extraction efficiency compared to Scenario 1, with reductions of 46% in greenhouse gas emissions and 48% in resource depletion. Overall, the findings highlight the potential of integrating innovative, energy-efficient technologies for the sustainable valorization of juice industry waste, offering measurable environmental advantages for industrial-scale implementation. Full article
(This article belongs to the Special Issue Agri-Food Wastes and Biomass Valorization—2nd Edition)
Show Figures

Figure 1

19 pages, 1560 KB  
Article
Integrated Framework to Assess Advanced Phosphorus Recycling as a Sustainable Alternative to Sewage Sludge in Agricultural Soils
by Juan Serrano-Gomez, Henrique Rasera Raniro, Ludwig Hermann, Manuel Pulido-Velazquez and Matthias Zessner
Waste 2025, 3(4), 41; https://doi.org/10.3390/waste3040041 - 27 Nov 2025
Viewed by 244
Abstract
Advanced phosphorus (P) recycling from wastewater is critical for improving nutrient circularity and reducing soil pollution associated with the direct application of sewage sludge in agriculture. However, few studies evaluate the long-term environmental and economic trade-offs between recycled P products and raw sewage [...] Read more.
Advanced phosphorus (P) recycling from wastewater is critical for improving nutrient circularity and reducing soil pollution associated with the direct application of sewage sludge in agriculture. However, few studies evaluate the long-term environmental and economic trade-offs between recycled P products and raw sewage sludge application. This study compares struvite, vivianite, and dicalcium phosphate (CaP) as P alternatives to sludge to mitigate heavy metal accumulation in Spanish agricultural soils. Using data from 27,835 plots, heavy metal accumulation was simulated over 50- and 100-year fertilisation scenarios. The results indicate that continuous sludge application leads to widespread exceedances of zinc, copper, and cadmium, especially in alkaline soils, whereas substitution with recycled products can substantially reduce these risks. Vivianite balances P recycling and costs, CaP offers the best environmental performance but with higher investment, and struvite suits smaller regions prioritising environmental safety. Economic analysis favours advanced recycling over sludge, especially considering externalities such as soil remediation costs. Despite limitations, our findings emphasise the importance of integrating environmental externalities into economic assessments and the value of advanced P recycling for sustainable soil management. Full article
Show Figures

Figure 1

12 pages, 210 KB  
Article
Adoption of Solid Waste Compost in Paddy Farming: Insights from Sri Lanka’s Organic Farming Policy
by Chamila Jeewanee Fernando and Aramaki Toshiya
Waste 2025, 3(4), 40; https://doi.org/10.3390/waste3040040 - 20 Nov 2025
Viewed by 226
Abstract
The utilization of Solid Waste Compost (SWC) as an organic fertilizer (OF) in agriculture has garnered significant attention in recent years due to growing concerns about worsening waste management issues. This empirical study investigates paddy farmers’ perceptions of SWC under Sri Lanka’s organic [...] Read more.
The utilization of Solid Waste Compost (SWC) as an organic fertilizer (OF) in agriculture has garnered significant attention in recent years due to growing concerns about worsening waste management issues. This empirical study investigates paddy farmers’ perceptions of SWC under Sri Lanka’s organic farming policy and uniquely addresses its underexploited potential as an organic fertilizer. Data were collected from 254 respondents in the Attanagalla Divisional Secretariat Division via a structured questionnaire. Nine key performance indicators were established to evaluate SWC against other organic fertilizers considered for the study. Findings revealed that meeting the ‘required quantity’ OF was the most challenging aspect (91%) for organic paddy cultivation, while only 14.2% of paddy farmers were able to utilize SWC for paddy fields due to limited availability. Farmers appreciated SWC as the most effective in balancing pest–predator interactions, even surpassing straw; however, its availability lagged compared to alternatives such as straw. Farmers expressed a higher likelihood of adopting SWC if it met government certification standards. The findings conclude that, while increasing production of SWC could enhance its role as an organic fertilizer in paddy farming, achieving its quality standards for paddy farming through government standard certification is crucial for successful implementation. Full article
(This article belongs to the Topic Advances in Organic Solid Waste and Wastewater Management)
19 pages, 1074 KB  
Systematic Review
From Trash to Treasure: Systematic Evaluation of Potential and Efficiency of Waste-to-Energy Incineration for Electricity Generation
by Nontobeko Gloria Maphuhla and Opeoluwa Oyehan Oyedeji
Waste 2025, 3(4), 39; https://doi.org/10.3390/waste3040039 - 17 Nov 2025
Viewed by 1854
Abstract
The massive production of municipal solid waste presents a significant global challenge for sustainable urban development and maintaining citizens’ quality of life, requiring effective management and disposal strategies. Waste-to-energy incineration technology has become increasingly important as a solution that simultaneously addresses the growing [...] Read more.
The massive production of municipal solid waste presents a significant global challenge for sustainable urban development and maintaining citizens’ quality of life, requiring effective management and disposal strategies. Waste-to-energy incineration technology has become increasingly important as a solution that simultaneously addresses the growing volumes of municipal solid waste and rising energy needs worldwide. This comprehensive review examines the research findings on the effectiveness of incineration as a waste-to-energy conversion method. The primary goal was to conduct a thorough systematic review assessing WtE incineration effectiveness across several key areas: energy recovery efficiency, waste volume reduction capabilities, environmental impact, and economic feasibility. A comprehensive literature search was conducted across ScienceDirect and additional pertinent databases, utilizing appropriate search terms in accordance with the PRISMA framework. A total of 431 studies were systematically identified, published between 2015 and 2025, and only 25 relevant studies were included in this review. Researchers collected data focusing on energy recovery percentages, volume reduction rates, emission reductions, and economic performance metrics. The findings revealed that every study included in the analysis showed positive results for WtE incineration across various performance measures. This research discovered the feasibility of generating electrical power from garbage through WtE incineration processes. The projected energy yields, ranging from gigawatt-hours to kilowatt-hours, were quantified for several nations, including Mexico (11,681.64 GWh), Cambodia (1625.81 GWh), Bangladesh (187.04 GWh), South Africa (6944 GWh), Iran (17,678 GWh), Nigeria (10,000 GWh), Indonesia (2487 MWh), Algeria (11.6 MWh), China (2316.52 MWh), Iraq (203.917 MWh), Uganda (774 kWh), and Pakistan (675 kWh). Energy recovery efficiency demonstrated a wide range from 30% to 92.75%, with waste volume reduction consistently reaching 90–95% levels, significantly prolonging landfill operational lifespans. From an environmental perspective, technology achieved greenhouse gas emission reductions ranging from 30% to 87%. This dual-purpose approach makes it an attractive, sustainable solution for both waste management and renewable energy production. By adopting this approach, cities can address waste and energy issues while boosting economic growth and job creation. However, it also involves substantial costs, technical difficulties, and environmental hazards that necessitate meticulous oversight. Full article
Show Figures

Figure 1

20 pages, 4458 KB  
Article
Proliferation of Plastic Packaging and Its Environmental Impacts at the Commune of Agoè-Nyivé 4 in Togo
by Ibrahim Batcham, Djiwonou Koffi Adjalo, Koko Zébéto Houedakor, Komlan Kounon Etienne Tede and Kossiwa Zinsou-Klassou
Waste 2025, 3(4), 38; https://doi.org/10.3390/waste3040038 - 3 Nov 2025
Viewed by 830
Abstract
The overconsumption of plastic packaging has alarming repercussions on the environment, notably through waste accumulation in public spaces and clogged drains. This study identifies factors driving plastic proliferation, analyzes their impacts, and proposes strategies for sustainable waste management. A cross-sectional design combined document [...] Read more.
The overconsumption of plastic packaging has alarming repercussions on the environment, notably through waste accumulation in public spaces and clogged drains. This study identifies factors driving plastic proliferation, analyzes their impacts, and proposes strategies for sustainable waste management. A cross-sectional design combined document review, field observations, and interviews with 156 households and 24 informants. Descriptive statistics characterized consumption patterns and service access. Impacts were assessed through litter hotspots, blocked drains, flood-prone points, and reported health risks. Households used five to six plastic bags daily, while collection coverage remained below 50%, sustaining persistent leakage. Findings reveal excessive reliance on plastics, shaped by technical, social, and institutional gaps, including weak segregation and limited pre-collection. Agoè-Nyivé 4, a fast-growing peri-urban commune within Greater Lomé, faces limited services but high consumption, making it a relevant case for rapidly growing municipalities. Yet the population often adopts counterproductive practices, hampering responsible waste management. A policy mix is outlined: expanding pre-collection and door-to-door services, integrating informal collectors, and targeted community sensitization. Without urgent interventions, plastic leakage will intensify environmental degradation, flooding, and health risks. The study recommends integrated policy measures to curb single-use dependence and foster a local circular economy. Full article
Show Figures

Figure 1

28 pages, 2764 KB  
Review
Cellulose-Based Biopolymers from Banana Pseudostem Waste: Innovations for Sustainable Bioplastics
by Alice Waithaka, Sofia Plakantonaki, Kyriaki Kiskira, Ann W. Mburu, Ioannis Chronis, Georgios Zakynthinos, John Githaiga and Georgios Priniotakis
Waste 2025, 3(4), 37; https://doi.org/10.3390/waste3040037 - 25 Oct 2025
Viewed by 2136
Abstract
Plastic materials are widely used for packaging due to their versatility and availability. Global production, mainly from petrochemicals, is estimated at 380 million tons, increasing annually by 4%. Packaging plastics have the shortest lifespan and contribute significantly to environmental pollution. Current production, use, [...] Read more.
Plastic materials are widely used for packaging due to their versatility and availability. Global production, mainly from petrochemicals, is estimated at 380 million tons, increasing annually by 4%. Packaging plastics have the shortest lifespan and contribute significantly to environmental pollution. Current production, use, and disposal of these plastics harm the environment, hu-mans, and ecosystems. Microplastics, (plastics particles ranging from 1 µm to 5 mm) formed through degradation, accumulate in ecosystems and the human body, including the brain. Bioplastics and biodegradable polymers from biological sources are a sustainable alternative; however, most production still relies on food crops, raising concerns about food security and sustainability. Utilizing organic wastes reduces production costs, lessens pressure on food systems, and supports waste management efforts. Cellulose, an abundant natural polymer, offers strong potential due to biodegradability, availability, and mechanical properties. This review explores extracting cellulose from banana pseudostem waste for packaging, high-lighting extraction and conversion methods and characterization via FTIR, TGA, SEM, XRD, and mechanical testing. FTIR confirmed the effective removal of lignin and hemicellulose, XRD revealed increased crystallinity corresponding to Type I cellulose, SEM showed a roughened fiber surface after alkaline treatment, and TGA indicated high thermal stability up to 250 °C. The goal is eco-friendly packaging by promoting agrowaste use. Further research should improve performance and scalability of cellulose-based bioplastics to meet industry needs and compete effectively with conventional plastics. Full article
(This article belongs to the Special Issue Agri-Food Wastes and Biomass Valorization—2nd Edition)
Show Figures

Figure 1

17 pages, 794 KB  
Article
Life Cycle Assessment of Reconditioned Guardrail Beams
by Daniel Mattos, Joaquim C. G. Esteves da Silva and Luis Pinto da Silva
Waste 2025, 3(4), 36; https://doi.org/10.3390/waste3040036 - 22 Oct 2025
Viewed by 384
Abstract
Steel consumption in the construction sector is one of the main contributors to global greenhouse gas emissions. Therefore, developing processes for the reuse of steel-based products with lower environmental impacts is essential for the sustainability of the construction sector. One example is the [...] Read more.
Steel consumption in the construction sector is one of the main contributors to global greenhouse gas emissions. Therefore, developing processes for the reuse of steel-based products with lower environmental impacts is essential for the sustainability of the construction sector. One example is the reuse of metal road guardrail beams on highways. This study investigated the environmental sustainability of a reconditioning process for such beams, instead of using new guardrails. The environmental impacts of the process were studied and compared with the impacts of the traditional production process using a Life Cycle Assessment (LCA) approach. This study revealed that most of the impacts of the reconditioning process derive from the use of electricity. The comparison with the traditional beam production process revealed that when primary raw materials are replaced by reused raw materials, the environmental impacts associated with the production process decrease significantly. Of the 19 impact indicators assessed, 18 were lower, and 17 had a drop of more than 90 percent compared to the traditional production process. The results indicate that the reconditioning process has the potential to significantly reduce environmental impacts by avoiding the consumption and transportation of primary raw materials, which were identified as the main sources of impacts in the traditional production process, as well as minimizing waste generation. Full article
(This article belongs to the Special Issue Use of Waste Materials in Construction Industry)
Show Figures

Figure 1

40 pages, 3822 KB  
Article
Physico-Mechanical Properties of an Aluminosilicate Refractory Castable Obtained After Chamotte Waste Recycling by Firing Method
by Leonel Díaz-Tato, Jesús Fernando López-Perales, Yadira González-Carranza, José Eulalio Contreras de León and Edén Amaral Rodríguez-Castellanos
Waste 2025, 3(4), 35; https://doi.org/10.3390/waste3040035 - 17 Oct 2025
Cited by 3 | Viewed by 1294
Abstract
Developing sustainable ceramic formulations that integrate industrial by-products addresses the high energy and raw material demands of refractory manufacturing while advancing circular economy goals. This study investigates the recycling of chamotte waste from rejected fired electrical porcelain as a partial substitute (5 and [...] Read more.
Developing sustainable ceramic formulations that integrate industrial by-products addresses the high energy and raw material demands of refractory manufacturing while advancing circular economy goals. This study investigates the recycling of chamotte waste from rejected fired electrical porcelain as a partial substitute (5 and 10 wt.%) for flint clay in aluminosilicate refractory castables. Samples were fired at 110, 815, 1050, and 1400 °C and evaluated for bulk density, apparent porosity, cold crushing strength, and flexural strength. Microstructural and mineralogical changes were analyzed by SEM and XRD. Incorporating 10 wt.% chamotte waste fostered an in situ mullite-reinforced microstructure, enhancing mechanical strength (58 MPa—CCS, 18.8 MPa—MOR) and lowering porosity (24.4%), demonstrating chamotte’s dual role as recycled raw material and reinforcement phase for densification and durability. These properties matched or surpassed those of the conventional formulation, with strength improvements of up to 44%. The findings demonstrate that high-temperature industrial waste can be effectively valorized in advanced refractories, reducing reliance on virgin raw materials, diverting waste from landfills, and promoting industrial symbiosis within the ceramics and metallurgical sectors. Full article
Show Figures

Graphical abstract

12 pages, 1596 KB  
Article
Impact on the Rheological Properties and Amino Acid Compositions of the Industrial Evaporation of Waste Vinasse in the Production of Nutritional Supplements for Livestock
by Nayeli Gutiérrez-Casiano, Cesar Antonio Ortíz-Sánchez, Karla Díaz-Castellanos, Luis Antonio Velázquez-Herrera, Solmaría Mandi Pérez-Guzmán and Eduardo Hernández-Aguilar
Waste 2025, 3(4), 34; https://doi.org/10.3390/waste3040034 - 2 Oct 2025
Viewed by 612
Abstract
Vinasse a byproduct of ethanol manufacturing, is a challenge for ethanol producers which possesses a high organic content that presents a considerable environmental threat. This complicates its management and treatment utilizing standard technologies like anaerobic digestion. This residue contains a substantial quantity of [...] Read more.
Vinasse a byproduct of ethanol manufacturing, is a challenge for ethanol producers which possesses a high organic content that presents a considerable environmental threat. This complicates its management and treatment utilizing standard technologies like anaerobic digestion. This residue contains a substantial quantity of dead and lysed yeast cells, which can function as a protein source for livestock’s nutritional needs. The application of multi-effect evaporation enhances the characteristics of this residue by increasing protein concentration, reducing volume, and minimizing water content. This study examines the impact of the five-effect evaporation procedure on vinasse waste, focusing on its rheological properties and the concentrations of proteins, amino acids, RNA, and DNA. This study aims to assess the thermal impacts linked to the evaporation process. The findings of the one-way statistical analysis demonstrate that the five evaporation effects are relevant in the utilization of waste as feed for livestock. The substance has a viscosity of 0.933 Pa s, comprising 6.3 g/100 g of crude protein, 4.08 g/100 g of amino acids, 0.1158 g/L of DNA, and 0.1031 g/L of RNA. Full article
Show Figures

Figure 1

22 pages, 3763 KB  
Article
Industrial Food Waste Screening in Emilia-Romagna and the Conceptual Design of a Novel Process for Biomethane Production
by Antonio Conversano, Samuele Alemanno, Davide Sogni and Daniele Di Bona
Waste 2025, 3(4), 33; https://doi.org/10.3390/waste3040033 - 30 Sep 2025
Viewed by 542
Abstract
The REPowerEU plan is aimed at a target of 35 bcm of biomethane annually by 2030, up from 4 bcm in 2023, requiring about EUR 37 billion in investment. Food waste is identified as a key feedstock, characterized by discrete homogeneity, although its [...] Read more.
The REPowerEU plan is aimed at a target of 35 bcm of biomethane annually by 2030, up from 4 bcm in 2023, requiring about EUR 37 billion in investment. Food waste is identified as a key feedstock, characterized by discrete homogeneity, although its availability may vary seasonally. In Italy, the Emilia-Romagna region generates approximately 450 kt/y of industrial waste from the food and beverage sector, primarily originating from meat processing (NACE 10.1), fruit and vegetable processing (NACE 10.3), and the manufacture of vegetable and animal oils and fats (NACE 10.4). Of this amount, food and beverage processing waste (EWC 02) accounts for about 302 kt from NACE 10 (food, year 2019) and 14 kt from NACE 11 (beverage, year 2019). This study provides a comprehensive screening of waste streams generated by the local food and beverage industry in Emilia-Romagna, evaluating the number of enterprises, their value added, and recorded waste production. The screening led to the identification of suitable streams for further valorization strategies: a total of ~93 kt/y was selected for the preliminary conceptual design of an integrated process combining anaerobic digestion with hydrothermal treatment, aimed at supporting national biomethane production targets while maximizing material recovery through hydrochar production. Preliminary estimations indicate that the proposed process may achieve a biochemical methane potential of approximately 0.23 Nm3/kgVS, along with a hydrochar yield of about 130 kg/twaste. Full article
(This article belongs to the Special Issue New Trends in Liquid and Solid Effluent Treatment)
Show Figures

Figure 1

15 pages, 694 KB  
Article
Mechanical Performance and Durability of Concretes with Partial Replacement of Natural Aggregates by Construction and Demolition Waste
by Thamires Alves da Silveira, Rafaella dos Passos Nörnberg, Marcelo Subtil Santi, Renata Rabassa Morales, Alessandra Buss Tessaro, Hebert Luis Rosseto, Rafael de Avila Delucis and Guilherme Hoehr Trindade
Waste 2025, 3(4), 32; https://doi.org/10.3390/waste3040032 - 30 Sep 2025
Viewed by 617
Abstract
This study investigated the mechanical performance and durability of concretes produced with varying proportions of recycled coarse aggregate from construction and demolition waste (CDW), ranging from 0% to 100% replacement of natural coarse aggregate, using recycled aggregates derived from crushed concrete and mortar [...] Read more.
This study investigated the mechanical performance and durability of concretes produced with varying proportions of recycled coarse aggregate from construction and demolition waste (CDW), ranging from 0% to 100% replacement of natural coarse aggregate, using recycled aggregates derived from crushed concrete and mortar debris, characterized by lower density and high water absorption (~9%) compared to natural aggregates. A key contribution of this research lies in the inclusion of intermediate replacement levels (20%, 25%, 45%, 50%, and 65%), which are less explored in the literature and allow a more refined identification of performance thresholds. Fresh-state parameters (slump), axial compressive strength (7 and 28 days), total immersion water absorption, sorptivity, and chloride ion penetration depth (after 90 days of immersion in a 3.5% NaCl solution) were evaluated. The results indicate that, up to 50% CDW content, the concrete maintains slump (≥94 mm), characteristic strength (≥37.2 MPa at 28 days), and chloride penetration (≤14.1 mm) within the limits for moderate exposure conditions, in accordance with ABNT: NBR 6118. Water absorption doubled from 4.5% (0% CDW) to 9.5% (100% CDW), reflecting the higher porosity and adhered mortar on the recycled aggregate, which necessitates adjustments to the water–cement ratio and SSD pre-conditioning to preserve workability and minimize sorptivity. Concretes with more than 65% CDW exhibited chloride penetration depths exceeding 15 mm, potentially compromising durability without additional mitigation. The judicious incorporation of CDW, combined with optimized mix design practices and the use of supplementary cementitious materials (SCMs), demonstrates technical viability for reducing environmental impacts without significantly impairing the structural performance or service life of the concrete. Full article
(This article belongs to the Special Issue Use of Waste Materials in Construction Industry)
Show Figures

Graphical abstract

17 pages, 810 KB  
Article
Characterisation and Fertiliser Potential of Mechanically Dewatered Faecal Sludge from Anaerobic Digestion
by Dennis Ofori-Amanfo, Eugene Appiah-Effah, Barbara Gyapong-Korsah, Esi Awuah, Helen M. K. Essandoh, Miriam Appiah-Brempong and Issahaku Ahmed
Waste 2025, 3(4), 31; https://doi.org/10.3390/waste3040031 - 29 Sep 2025
Viewed by 786
Abstract
While mechanical dewatering is widely used in faecal sludge treatment, the agricultural potential of mechanically dewatered faecal sludge (MDFS) combined with anaerobic digestion (AD) remains underexplored, particularly in sub-Saharan Africa where nutrient recovery is critical for food security. This study provides the first [...] Read more.
While mechanical dewatering is widely used in faecal sludge treatment, the agricultural potential of mechanically dewatered faecal sludge (MDFS) combined with anaerobic digestion (AD) remains underexplored, particularly in sub-Saharan Africa where nutrient recovery is critical for food security. This study provides the first comprehensive characterisation of MDFS from Ghana’s largest treatment facility and evaluates anaerobic digestion effectiveness for agricultural application. Over six months, 182 composite MDFS samples from Lavender Hill Faecal Treatment Plant were analysed for physicochemical properties, nutrients, heavy metals, and microbial contaminants before and after AD treatment. MDFS demonstrated exceptional nutrient density, with total nitrogen (2141.05 mg/kg), phosphorus (190.08 mg/kg), and potassium (4434.88 mg/kg) concentrations comparable to commercial organic fertilisers. AD achieved significant pathogen reduction, decreasing total coliforms from 148,808.70 to 493.33 cfu/100 g (p < 0.001) and Ascaris lumbricoides eggs from 12.08 to 3.33 eggs/L, while maintaining nutrient integrity and keeping heavy metals within safe agricultural limits. Statistical modelling revealed a significant correlation between treatment duration and pathogen reduction efficiency. Despite substantial improvements, treated MDFS still exceeded some regulatory thresholds, indicating a need for complementary post-treatment strategies. This research establishes AD as an effective primary treatment for converting MDFS into a nutrient-rich organic fertiliser, supporting circular economy principles in urban sanitation systems and providing a sustainable pathway for agricultural nutrient recovery in resource-constrained settings. Full article
Show Figures

Figure 1

16 pages, 1260 KB  
Article
Trichoderma harzianum Enzyme Production in Stirred Solid-State Bioreactors as a Strategy for Valorizing Water Hyacinth
by Nohemi López-Ramírez, Ernesto Favela-Torres, Tania Volke-Sepúlveda and Fernando Méndez-González
Waste 2025, 3(4), 30; https://doi.org/10.3390/waste3040030 - 25 Sep 2025
Viewed by 868
Abstract
Water hyacinth is an invasive weed that can valorize through the production of hydrolytic enzymes via solid-state culture. This study explores the application of Trichoderma harzianum in producing xylanases and endoglucanases on water hyacinth beds. Laboratory-scale packed-bed column bioreactors (PBCBs) with a capacity [...] Read more.
Water hyacinth is an invasive weed that can valorize through the production of hydrolytic enzymes via solid-state culture. This study explores the application of Trichoderma harzianum in producing xylanases and endoglucanases on water hyacinth beds. Laboratory-scale packed-bed column bioreactors (PBCBs) with a capacity of 8 grams of dry mass (gdm) were used to evaluate the effects of temperature (28–36 °C) and initial moisture content (65–80%) on microbial growth and enzyme production. High yields of biomass and enzymes were produced at 30 °C. Moreover, xylanase activity was enhanced in cultures with a moisture content of 65% (~71.24 U/gdm), and endoglucanase activity at 75–80% moisture (~20.13 U/gdm). The operational conditions identified for xylanase production were applied to 6 L bench-scale cross-flow internally stirred bioreactors, packed to 40% capacity with 450 gdm. Two stirring regimes were tested: intermittent and continuous. The results showed that continuous stirring promotes both microbial growth and xylanase activity. In fact, xylanase activity in continuous stirring conditions was comparable to that achieved in PBCBs. Consequently, continuous stirring enables a 56-fold increase in bioreactor capacity without compromising xylanase production. The approaches developed in this study can support the design of large-scale bioprocesses for the valorization of water hyacinth. Full article
Show Figures

Graphical abstract

Previous Issue
Next Issue
Back to TopTop