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BioTech, Volume 10, Issue 4

2021 December - 10 articles

Cover Story: A stimulating effect on protein synthesis was detected with extracts prepared from recombinant cells, in which the E. coli RNA polymerase subunits α, β, β’ and ω are simultaneously coexpressed. Appending a 3′ UTR genomic sequence and a T7 transcription terminator to the protein-coding region also improves the synthetic activity of some genes from linear DNA. The E. coli BL21 (DE3) rna::Tn10 mutant deficient in a periplasmic RNase I was constructed. The mutant cell-free extract increases by up to four-fold the expression of bacterial and human genes mediated from both bacterial pargC and phage pT7 promoters. By contrast, the RNase E deficiency does not affect the cell-free expression of the same genes. View this paper.
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Articles (10)

  • Article
  • Open Access
5 Citations
7,081 Views
14 Pages

13 December 2021

The present study explores the pressing matter of mandatory vaccination in Europe from an ethical–constitutional perspective. To start with, it considers the bases of the concerns that have been raised to date, as well as those of the documente...

  • Article
  • Open Access
9 Citations
5,914 Views
17 Pages

3 December 2021

The societal acceptability of different applications of genomic technologies to animal production systems will determine whether their innovation trajectories will reach the commercialisation stage. Importantly, technological implementation and comme...

  • Article
  • Open Access
20 Citations
4,938 Views
14 Pages

Drought Resistance Loci in Recombinant Lines of Iranian Oryza sativa L. in Germination Stage

  • Morteza Noryan,
  • Islam Majidi Hervan,
  • Hossein Sabouri,
  • Faroukh Darvish Kojouri and
  • Andrea Mastinu

6 November 2021

In order to locate control genes related to Oryza sativa L. traits at the germination stage under normal conditions and at drought stress levels (−4.5 and −9.0 bar), we evaluated 120 F8 generation offspring from the cross between two cultivars Neda ×...

  • Article
  • Open Access
7 Citations
5,887 Views
13 Pages

VirusLab: A Tool for Customized SARS-CoV-2 Data Analysis

  • Pietro Pinoli,
  • Anna Bernasconi,
  • Anna Sandionigi and
  • Stefano Ceri

6 November 2021

Since the beginning of 2020, the COVID-19 pandemic has posed unprecedented challenges to viral data analysis and connected host disease diagnostic methods. We propose VirusLab, a flexible system for analysing SARS-CoV-2 viral sequences and relating t...

  • Communication
  • Open Access
4 Citations
4,641 Views
8 Pages

In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique

  • Arik Makovitzki,
  • Avital Jayson,
  • Ziv Oren,
  • Elad Lerer,
  • Yaron Kafri,
  • Eyal Dor,
  • Lilach Cherry,
  • Hanan Tzadok,
  • Lilach Levin and
  • Osnat Rosen
  • + 4 authors

3 November 2021

The COVID-19 pandemic caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) increases the need for a rapid development of efficient vaccines. Among other vaccines in clinical trials, a recombinant VSV-∆G-spike vaccine was devel...

  • Article
  • Open Access
2 Citations
5,071 Views
15 Pages

Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery

  • Marina Snapyan,
  • Sylvain Robin,
  • Garabet Yeretssian,
  • Michèle Lecocq,
  • Frédéric Marc and
  • Vehary Sakanyan

14 October 2021

We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophil...

  • Article
  • Open Access
20 Citations
5,901 Views
19 Pages

Primer Binding Site (PBS) Profiling of Genetic Diversity of Natural Populations of Endemic Species Allium ledebourianum Schult.

  • Oxana Khapilina,
  • Ainur Turzhanova,
  • Alevtina Danilova,
  • Asem Tumenbayeva,
  • Vladislav Shevtsov,
  • Yuri Kotukhov and
  • Ruslan Kalendar

13 October 2021

Endemic species are especially vulnerable to biodiversity loss caused by isolation or habitat specificity, small population size, and anthropogenic factors. Endemic species biodiversity analysis has a critically important global value for the develop...

  • Article
  • Open Access
19 Citations
7,998 Views
12 Pages

Highly Efficient Purification of Recombinant VSV-∆G-Spike Vaccine against SARS-CoV-2 by Flow-Through Chromatography

  • Elad Lerer,
  • Ziv Oren,
  • Yaron Kafri,
  • Yaakov Adar,
  • Einat Toister,
  • Lilach Cherry,
  • Edith Lupu,
  • Arik Monash,
  • Rona Levy and
  • Arik Makovitzki
  • + 6 authors

12 October 2021

This study reports a highly efficient, rapid one-step purification process for the production of the recombinant vesicular stomatitis virus-based vaccine, rVSV-∆G-spike (rVSV-S), recently developed by the Israel Institute for Biological Research (IIB...

  • Review
  • Open Access
18 Citations
8,438 Views
15 Pages

11 October 2021

Many techniques are currently in use to study microbes. These can be aimed at detecting, identifying, and characterizing bacterial, fungal, and viral species. One technique that is suitable for high-throughput analysis is flow cytometry-based fluores...

  • Communication
  • Open Access
10 Citations
6,006 Views
14 Pages

Searching for G-Quadruplex-Binding Proteins in Plants: New Insight into Possible G-Quadruplex Regulation

  • Adriana Volná,
  • Martin Bartas,
  • Jakub Nezval,
  • Vladimír Špunda,
  • Petr Pečinka and
  • Jiří Červeň

22 September 2021

G-quadruplexes are four-stranded nucleic acid structures occurring in the genomes of all living organisms and viruses. It is increasingly evident that these structures play important molecular roles; generally, by modulating gene expression and overa...

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BioTech - ISSN 2673-6284