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Methods and Protocols, Volume 7, Issue 4

2024 August - 16 articles

Cover Story: This study assessed the use of X-ray fluorescence (XRF) to analyse multi-elemental soil and plant materials by examining certified reference samples. A portable XRF analyzer evaluated 32 soil and 12 plant standards using the Soil and Geochem modes with sequential beams to detect various elements. Recovery rates were calculated by comparing XRF measurements to certified values, with correlations verified through the Spearman coefficient. The results showed good reliability for soil samples, while XRF overestimated plant values, which could be corrected using a division factor. XRF may be a viable alternative to ICP-MS for routine analysis. View this paper
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Articles (16)

  • Protocol
  • Open Access
1,588 Views
11 Pages

From 2015 to 2023, we conducted a comprehensive study in the 11,893-hectare hunting area managed by the Marcal-Bitvaközi Hunting Company, characterised by its substantial wild boar population. The research was carried out across various settings...

  • Article
  • Open Access
2,202 Views
15 Pages

3-indoxyl sulfate (3-IS) results from a hepatic transformation of indole, a tryptophan degradation product produced by commensal gut bacteria. The metabolite has shown promise as a biomarker of dysbiosis and clinical outcomes following hematopoietic...

  • Article
  • Open Access
2,717 Views
19 Pages

A Mathematical Model of Pressure Ulcer Formation to Facilitate Prevention and Management

  • Ioannis G. Violaris,
  • Konstantinos Kalafatakis,
  • Nikolaos Giannakeas,
  • Alexandros T. Tzallas and
  • Markos Tsipouras

Pressure ulcers are a frequent issue involving localized damage to the skin and underlying tissues, commonly arising from prolonged hospitalization and immobilization. This paper introduces a mathematical model designed to elucidate the mechanics beh...

  • Protocol
  • Open Access
3 Citations
2,745 Views
19 Pages

Evaluation of an Italian Population-Based Programme for Risk Assessment and Genetic Counselling and Testing for BRCA1/2-Related Hereditary Breast and Ovarian Cancer after 10 Years of Operation: An Observational Study Protocol

  • Stefano Ferretti,
  • Priscilla Sassoli de Bianchi,
  • Debora Canuti,
  • Cinzia Campari,
  • Laura Cortesi,
  • Valentina Arcangeli,
  • Elena Barbieri,
  • Cecilia D’Aloia,
  • Rita Danesi and
  • the HBOC Study Group
  • + 14 authors

Hereditary breast/ovarian cancer (HBOC) syndrome is caused by the inheritance of monoallelic germline BRCA1/2 gene mutations. If BRCA1/2 mutation carriers are identified before the disease develops, effective actions against HBOC can be taken, includ...

  • Protocol
  • Open Access
3 Citations
2,660 Views
16 Pages

The Sri Lanka Mother and Newborn Growth (S-MaNGro) Cohort: Protocol of a Nationwide Prospective Study

  • Malshani Lakshika Pathirathna,
  • Megumi Haruna,
  • Satoshi Sasaki,
  • Kaori Yonezawa,
  • Yuriko Usui and
  • Yasuhiro Hagiwara

Perinatal cohort studies with a prospective longitudinal design are critical for determining the effects of early-life exposures on offspring’s health outcomes. The Sri Lanka Mother and Newborn Growth cohort study aims to investigate the impact...

  • Article
  • Open Access
4 Citations
2,552 Views
12 Pages

Phytochemical Composition and Antimicrobial and Antibiofilm Effect of Myrciaria cauliflora Hydroethanolic Extract against Staphylococcus aureus and Acinetobacter baumannii

  • Luciane Dias de Oliveira,
  • Ana Luisa Monteiro Ribeiro,
  • Sthéfani de Oliveira Dias,
  • Geovani Moreira da Cruz,
  • Raquel Teles de Menezes,
  • Lara Steffany de Carvalho,
  • Mariana Gadelho Gimenez Diamantino,
  • Thaís Cristine Pereira,
  • Maria Cristina Marcucci and
  • Amjad Abu Hasna

Staphylococcus aureus and Acinetobacter baumannii are opportunistic pathogens, and both are involved in different oral infections. This work aimed to analyze the phytochemical composition of Myrciaria cauliflora hydroethanolic extract and to evaluate...

  • Protocol
  • Open Access
1 Citations
1,573 Views
15 Pages

This study focuses on the development and optimization of a water removal device for biogenic volatile organic compounds (BVOCs) from plant emissions. BVOCs play a crucial role in various ecological processes and have potential therapeutic effects on...

  • Study Protocol
  • Open Access
1 Citations
2,055 Views
10 Pages

Unresectable, metastatic, advanced urothelial carcinoma (aUC) is an aggressive disease and is treated with platinum-containing first-line chemotherapy, followed by immune checkpoint inhibitors and antibody–drug conjugates. Response to first-lin...

  • Protocol
  • Open Access
2,607 Views
8 Pages

With the advent of “next-generation” sequencing and the continuous reduction in sequencing costs, an increasing amount of genomic data has emerged, such as whole-genome, whole-exome, and targeted sequencing data. These applications are po...

  • Article
  • Open Access
2,260 Views
17 Pages

Image Processing Analysis of Plant Morphometry with Examples from the Genus Sedum (Crassulaceae)

  • Mădălina Borcă,
  • Alexandru Borcă,
  • Alin Ciobica,
  • Gabriela Halitchi and
  • Andrei Stoie

The complex systematics of the genus Sedum, the difficulties of its classification and the ambiguity of the concrete identification of the taxa brought about the need to implement a measurement system adaptable to field conditions, so as to facilitat...

  • Article
  • Open Access
3 Citations
3,352 Views
10 Pages

Odours in Asphalt: Analysis of the Release of H2S from Bitumen by a Mass Spectrometric Residual Gas Analyser

  • Marcello Monteleone,
  • Giuseppe Di Luca,
  • Marcello Filomia,
  • Alessio Fuoco,
  • Alberto Figoli and
  • Johannes Carolus Jansen

During the production and laying phases of hot-mixing asphalt (HMA), various volatile organic compounds (VOCs) and noxious gases such as H2S are released into the atmosphere. These emissions are a serious environmental problem, a risk to human health...

  • Study Protocol
  • Open Access
9 Citations
3,114 Views
10 Pages

Protocol for the Development and Initial Validation of the COG-IMPACT Tool: A Purpose-Built Unmet Needs Assessment for Cancer-Related Cognitive Impairment

  • Darren Haywood,
  • Moira O’Connor,
  • Frank D. Baughman,
  • Alexandre Chan,
  • Raymond J. Chan,
  • Evan Dauer,
  • Haryana M. Dhillon,
  • Ashley M. Henneghan,
  • Blake J. Lawrence and
  • Nicolas H. Hart
  • + 3 authors

(1) Background: A significant proportion of cancer survivors report experiencing a cognitive ‘fog’ that affects their ability to think coherently and quickly, and reason with clarity. This has been referred to as cancer-related cognitive...

  • Communication
  • Open Access
18 Citations
4,739 Views
12 Pages

This study investigated the suitability of X-ray fluorescence (XRF) analysis for routine multi-elemental composition analysis, checking its analytical capabilities by measuring a wide array of certified reference materials of soil and plant origin. A...

  • Review
  • Open Access
7 Citations
4,194 Views
16 Pages

Methods to Investigate the Secretome of Senescent Cells

  • Afshin Samiminemati,
  • Domenico Aprile,
  • Dario Siniscalco and
  • Giovanni Di Bernardo

The word “secretome” was first used to describe the proteins that cells secrete under different circumstances; however, recent studies have proven the existence of other molecules such as RNA and chemical compounds in the secretome. The s...

  • Review
  • Open Access
5 Citations
9,020 Views
16 Pages

A Review of Histological Techniques for Differentiating Human Bone from Animal Bone

  • Emanuela Stan,
  • Camelia-Oana Muresan,
  • Ecaterina Daescu,
  • Raluca Dumache,
  • Veronica Ciocan,
  • Stefania Ungureanu,
  • Dan Costachescu and
  • Alexandra Enache

The first step in anthropological study is the positive identification of human remains, which can be a challenging undertaking when bones are broken. When bone pieces from different species are mixed together, it can be crucial to distinguish betwee...

  • Protocol
  • Open Access
7 Citations
7,987 Views
12 Pages

Background: Essential oils have gained in significance due to their various biological activities, and there is a growing demand for them in many industries. The present article focuses on the technical steps for an in vitro evaluation of the antibac...

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Methods Protoc. - ISSN 2409-9279