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Article

Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis

1
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15898 Santiago de Compostela, Spain
2
Department of Food Technology, Spanish National Research Council, Marine Research Institute, 36208 Vigo, Spain
3
Agroalimentary Technological Center of Lugo, 27002 Lugo, Spain
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Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain
5
Department of Analytical Chemistry, Complutense University of Madrid, 28040 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Foods 2021, 10(4), 799; https://doi.org/10.3390/foods10040799
Received: 8 March 2021 / Revised: 28 March 2021 / Accepted: 6 April 2021 / Published: 8 April 2021
The present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected. View Full-Text
Keywords: pathogen detection; LC-ESI-MS/MS; proteomics; mass spectrometry; phage peptide biomarker pathogen detection; LC-ESI-MS/MS; proteomics; mass spectrometry; phage peptide biomarker
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MDPI and ACS Style

Abril, A.G.; Carrera, M.; Böhme, K.; Barros-Velázquez, J.; Cañas, B.; Rama, J.-L.R.; Villa, T.G.; Calo-Mata, P. Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis. Foods 2021, 10, 799. https://doi.org/10.3390/foods10040799

AMA Style

Abril AG, Carrera M, Böhme K, Barros-Velázquez J, Cañas B, Rama J-LR, Villa TG, Calo-Mata P. Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis. Foods. 2021; 10(4):799. https://doi.org/10.3390/foods10040799

Chicago/Turabian Style

Abril, Ana G., Mónica Carrera, Karola Böhme, Jorge Barros-Velázquez, Benito Cañas, José-Luis R. Rama, Tomás G. Villa, and Pilar Calo-Mata. 2021. "Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis" Foods 10, no. 4: 799. https://doi.org/10.3390/foods10040799

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