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Separations, Volume 10, Issue 3 (March 2023) – 76 articles
Coccidiosis is a very contagious disease that may result in economic losses, especially in intensive farms.
The development of multi-residue methods that are able to determine regulated compounds is still quite challenging, owing to their different physicochemical properties and the LODs that have to be reached. LC-MS/MS is currently the most used technique for their determination in feed and food. To the best of our knowledge, these analytes have only been determined in three cases by means of HRMS. The present work was carried out to obtain fit for purpose LODs and the highest possible selectivity of a method for the determination of coccidiostats in eggs and muscle with LC-Q-Orbitrap, performing parallel reaction monitoring (PRM) acquisition for the first time. Moreover, a full validation study was carried out according to CIR (EU) 2021/808. View this paper
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