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Open AccessFeature PaperArticle

Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf

1
School of Biosciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
2
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
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Research and Training Department, Graduate School, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
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Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
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School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500 Selangor Darul Ehsan, Malaysia
6
School of Molecular and Life Sciences, Curtin University, Bentley, GPO Box U1987, Perth, WA 6845, Australia
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Fundamental and Applied Sciences Department, Centre of Innovative Nanostructures and Nanodevices, Institute of Autonomous Systems, Universiti Teknologi PETRONAS, 32610 Bandar Seri Iskandar, Malaysia
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Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Processes 2020, 8(2), 247; https://doi.org/10.3390/pr8020247
Received: 10 January 2020 / Revised: 16 February 2020 / Accepted: 17 February 2020 / Published: 21 February 2020
(This article belongs to the Special Issue Biotechnology for Sustainability and Social Well Being)
Persiscaria tenulla, commonly known as Polygonum, is a plant belonging to the family Polygonaceae, which originated from and is widely found in Southeast Asia countries, such as Indonesia, Malaysia, Thailand, and Vietnam. The leaf of the plant is believed to have active ingredients that are responsible for therapeutic effects. In order to take full advantage of a natural medicinal plant for the application in the pharmaceutical and food industries, extraction and separation techniques are essential. In this study, an emerging and rapid extraction approach known as liquid biphasic flotation (LBF) is proposed for the extraction of protein from Persiscaria tenulla leaves. The scope of this study is to establish an efficient, environmentally friendly, and cost-effective technology for the extraction of protein from therapeutic leaves. Based on the ideal conditions of the small LBF system, a 98.36% protein recovery yield and a 79.12% separation efficiency were achieved. The upscaling study of this system exhibited the reliability of this technology for large-scale applications with a protein recovery yield of 99.44% and a separation efficiency of 93.28%. This technology demonstrated a simple approach with an effective protein recovery yield and separation that can be applied for the extraction of bioactive compounds from various medicinal-value plants. View Full-Text
Keywords: extraction; leaf; liquid biphasic flotation; polygonum; protein extraction; leaf; liquid biphasic flotation; polygonum; protein
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Saw, H.S.; Sankaran, R.; Khoo, K.S.; Chew, K.W.; Phong, W.N.; Tang, M.S.; Lim, S.S.; Mohd Zaid, H.F.; Naushad, M.; Show, P.L. Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf. Processes 2020, 8, 247.

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