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Antibiotics
Volume 14
Issue 3
10.3390/antibiotics14030297
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Article

Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds

by
Paola A. Rios Agudelo
1,
Julián Reyes Vélez
1,
Martha Olivera Angel
2,
Adam M. Blanchard
3,
Yesid Cuesta Astroz
1,4,
Arley Caraballo Guzmán
1 and
Giovanny Torres Lindarte
1,*
1
Instituto Colombiano de Medicina Tropical, Universidad CES, Cra 43a No. 52 Sur 99, Sabaneta 055450, Antioquia, Colombia
2
Biogenesis Research Group, Faculty of Agriculture Science, University of Antioquia, Cra 75 No. 65-87, Medellín 050010, Antioquia, Colombia
3
School of Veterinary and Science, University of Nottingham, Sutton Bonington Campus, Leicestireshire LE12 5RD, UK
4
Escuela de Microbiología, Universidad de Antioquia, Ciudad Universitaria, Calle 67 No 12 53-108, Medellín 050010, Antioquia, Colombia
*
Author to whom correspondence should be addressed.
Antibiotics 2025, 14(3), 297; https://doi.org/10.3390/antibiotics14030297
Submission received: 22 December 2024 / Revised: 13 January 2025 / Accepted: 19 February 2025 / Published: 12 March 2025
(This article belongs to the Special Issue Antimicrobial Resistance of Pathogens Isolated from Bovine Mastitis)
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Abstract

Introduction: Streptococcus uberis is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamental to understand its complex epidemiology and behavior against antimicrobials. Methods: A comparative genomic analysis of 10 S. uberis strains was performed and their antimicrobial susceptibility was assessed. Results: Ten different novel sequence types were found, and genes (tetM, tetO, patB, lnuC, lnuA, lsaE, ermB, ANT(6)-la) and mobile genetic elements previously associated with antimicrobial resistance (repUS43, ISSag2, and ISEnfa4) and virulence (315.2 phage) were detected. Additionally, our strains had the highest relative rate of recombination to mutation (8.3) compared to other S. uberis strains isolated from different continents (America: 7.7, Asia: 2.9, Europe: 5.4, and Oceania: 6.6). Most of the strains (80%) tested showed phenotypic resistance to clindamycin and 70% exhibited intermediate susceptibility to penicillin. Conclusions: The high heterogeneity of strains observed and the presence of genetic factors linked to antimicrobial resistance represent a challenge for the implementation and surveillance of measures focused on the control and elimination of this pathogen.
Keywords: bovine mastitis; Streptococcus uberis; whole genome sequencing; recombination events; mobile genetic elements bovine mastitis; Streptococcus uberis; whole genome sequencing; recombination events; mobile genetic elements

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MDPI and ACS Style

Rios Agudelo, P.A.; Reyes Vélez, J.; Olivera Angel, M.; Blanchard, A.M.; Cuesta Astroz, Y.; Caraballo Guzmán, A.; Torres Lindarte, G. Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds. Antibiotics 2025, 14, 297. https://doi.org/10.3390/antibiotics14030297

AMA Style

Rios Agudelo PA, Reyes Vélez J, Olivera Angel M, Blanchard AM, Cuesta Astroz Y, Caraballo Guzmán A, Torres Lindarte G. Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds. Antibiotics. 2025; 14(3):297. https://doi.org/10.3390/antibiotics14030297

Chicago/Turabian Style

Rios Agudelo, Paola A., Julián Reyes Vélez, Martha Olivera Angel, Adam M. Blanchard, Yesid Cuesta Astroz, Arley Caraballo Guzmán, and Giovanny Torres Lindarte. 2025. "Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds" Antibiotics 14, no. 3: 297. https://doi.org/10.3390/antibiotics14030297

APA Style

Rios Agudelo, P. A., Reyes Vélez, J., Olivera Angel, M., Blanchard, A. M., Cuesta Astroz, Y., Caraballo Guzmán, A., & Torres Lindarte, G. (2025). Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds. Antibiotics, 14(3), 297. https://doi.org/10.3390/antibiotics14030297

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