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Biosensors, Volume 15, Issue 6

June 2025 - 62 articles

Cover Story: We developed for the first time a functional immobilized CRISPR/Cas13a assay in a chitosan hydrogel-coated 3D-printed 96-well plate, enabling unamplified quantification of three distinct miRNAs simultaneously associated with Alzheimer’s disease, achieving femtomolar sensitivity with an LOD of 0.1 fM. This immobilization strategy enables multiplex detection, providing a simplified, efficient mix and read platform, removing complex sample preparation steps and eliminating specialized training requirements. Target binding to CRISPR RNA triggers Cas13a collateral RNase activity, cleaving a fluorescent reporter with real-time reaction monitoring. This cost-effective method (USD 3.5/plate) is validated in patient plasma samples exhibiting low cross-reactivity (<5%). It has great potential in detecting multiple pathogens and clinical disorders. View this paper
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Articles (62)

  • Article
  • Open Access
2 Citations
1,633 Views
22 Pages

14 June 2025

A novel nanocomposite based on electrochemically reduced graphene oxide (ERGO) and electropolymerized polyproflavine (PPFL) was obtained within a “one-pot” synthesis from natural deep eutectic solvent (NADES). NADES consisted of citric ac...

  • Article
  • Open Access
1 Citations
1,639 Views
15 Pages

13 June 2025

We are reporting the development of a high-performance, non-enzymatic electrochemical biosensor for selective lactate detection, integrating laser-induced graphene (LIG), gold nanoparticles (AuNPs), and a molecularly imprinted polymer (MIP) synthesiz...

  • Article
  • Open Access
711 Views
13 Pages

13 June 2025

Developing appropriate methods for real-time in situ investigation of how drugs influence signaling pathways and related biomolecules holds enormous potential for helping to provide an understanding of how anticancer drugs exert their effects. Herein...

  • Article
  • Open Access
3 Citations
1,261 Views
16 Pages

Sensing Protein Structural Transitions with Microfluidic Modulation Infrared Spectroscopy

  • Lathan Lucas,
  • Phoebe S. Tsoi,
  • Ananya Nair,
  • Allan Chris M. Ferreon and
  • Josephine C. Ferreon

13 June 2025

Microfluidic modulation spectroscopy-infrared (MMS) offers a label-free, high-sensitivity approach for quantifying changes in protein secondary structures under native solution conditions. MMS subtracts the solvent backgrounds from sample signals by...

  • Article
  • Open Access
1 Citations
875 Views
14 Pages

12 June 2025

Lower extremity lymphedema (LEL) requires precise limb measurements for treatment evaluation and compression garment design. Tape measurement (TM) is the standard method but is time-consuming. Smartphones with light detection and ranging (LiDAR) tech...

  • Review
  • Open Access
1 Citations
4,684 Views
36 Pages

Electrochemical Microneedles for Real-Time Monitoring in Interstitial Fluid: Emerging Technologies and Future Directions

  • Suhyeon Cha,
  • Min Yu Choi,
  • Min Jung Kim,
  • Sang Baek Sim,
  • Izzati Haizan and
  • Jin-Ha Choi

12 June 2025

Conventional blood-based detection methods for biomarkers and analytes face significant limitations, including complex processing, variability in blood components, and the inability to provide continuous monitoring. These challenges hinder the early...

  • Systematic Review
  • Open Access
1 Citations
2,909 Views
30 Pages

The Use of CRISPR-Cas Systems for Viral Detection: A Bibliometric Analysis and Systematic Review

  • Othmane Jeddoub,
  • Nadia Touil,
  • Omar Nyabi,
  • Elmostafa El Fahime,
  • Khalid Ennibi,
  • Jean-Luc Gala,
  • Abdelaziz Benjouad and
  • Lamiae Belayachi

12 June 2025

Viral infections impose a significant burden on global public health and the economy. This study examines the current state of CRISPR-Cas system research, focusing on their applications in viral detection and their evolution over recent years. A bibl...

  • Review
  • Open Access
4 Citations
3,091 Views
25 Pages

Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors

  • Yue Wu,
  • Xing Xu,
  • Yinchu Zhu,
  • Jiaxin Wan,
  • Xingbo Wang,
  • Xin Zhou,
  • Xiangjun Li and
  • Weidong Zhou

12 June 2025

The rapid and precise identification of multiple pathogens is critical for ensuring food safety, controlling epidemics, diagnosing diseases, and monitoring the environment. However, traditional detection methods are hindered by complex workflows, the...

  • Article
  • Open Access
1 Citations
956 Views
13 Pages

11 June 2025

Development of sensitive and convenient alkaline phosphatase (ALP) detection methods is of great significance for food analysis, biomedical applications, and clinical tests. In this work, a sensitive detection method for ALP was established based on...

  • Article
  • Open Access
2 Citations
1,065 Views
15 Pages

11 June 2025

Multimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode i...

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Biosensors - ISSN 2079-6374