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Antioxidants
Volume 8
Issue 10
10.3390/antiox8100494
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Article

Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro

by
Alessandro Attanzio
1,
Antonella D’Anneo
1,
Francesco Pappalardo
2,
Francesco Paolo Bonina
3,
Maria Antonia Livrea
1,
Mario Allegra
1,* and
Luisa Tesoriere
1
1
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, Università di Palermo, 90123 Palermo, Via Archirafi, Italy
2
Department Analytics of R&D, Bionap s.r.l., C.da Fureria, Zona Industriale Ovest, Piano Tavola, 95032 Belpasso (CT), Italy
3
Department of Pharmaceutical Science, Faculty of Pharmacy, Università di Catania, Viale Andrea Doria, 95125 Catania, Italy
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(10), 494; https://doi.org/10.3390/antiox8100494
Submission received: 4 October 2019 / Revised: 14 October 2019 / Accepted: 15 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Antioxidant and Anti-inflammatory Properties of Plants Extract)
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Abstract

Manna, a very singular vegetable product derived from the spontaneous solidification of the sap of some Fraxinus species, has long been known for its mild laxative and emollient properties. In this work, a hydro-alcoholic extract of manna (HME) from Sicilian Fraxinus angustifolia Vahl was investigated using HPLC-DAD to find phenol components and using chemical and biological in vitro assays to determine its reducing, antioxidant and anti-inflammatory capacity. We identified elenolic acid, tyrosol, hydroxytyrosol, catechin, fraxetin, verbascoside, gallic acid, procyanidin-B1, and luteolin 3,7 glucoside, in order of abundance. Measurements of total antioxidant activity by Folin-Ciocalteu reaction and ferric reducing ability (FRAP), as well as of scavenger activity towards ABTS•+, DPPH•, and perferryl-myoglobin radicals, showed that the phytocomplex effectively reduced oxidants with different standard potentials. When compared with vitamin E, HME also behaved as an efficient chain-breaking antioxidant against lipoperoxyl radicals from methyl linoleate. In cellular models for oxidative stress, HME counteracted membrane lipid oxidation of human erythrocytes stimulated by tert-butyl hydroperoxide and prevented the generation of reactive oxygen species, as well as the GSH decay in IL-1β–activated intestinal normal-like cells. Moreover, in this in vitro intestinal bowel disease model, HME reduced the release of the pro-inflammatory cytokines IL-6 and IL-8. These findings may suggest that manna acts as an antioxidant and anti-inflammatory natural product in humans, beyond its well-known effects against constipation.
Keywords: manna; Fraxinus; manna bioactivity; phytochemicals; antioxidant; reducing power; red blood cell oxidation; intestinal bowel disease model manna; Fraxinus; manna bioactivity; phytochemicals; antioxidant; reducing power; red blood cell oxidation; intestinal bowel disease model

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MDPI and ACS Style

Attanzio, A.; D’Anneo, A.; Pappalardo, F.; Bonina, F.P.; Livrea, M.A.; Allegra, M.; Tesoriere, L. Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro. Antioxidants 2019, 8, 494. https://doi.org/10.3390/antiox8100494

AMA Style

Attanzio A, D’Anneo A, Pappalardo F, Bonina FP, Livrea MA, Allegra M, Tesoriere L. Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro. Antioxidants. 2019; 8(10):494. https://doi.org/10.3390/antiox8100494

Chicago/Turabian Style

Attanzio, Alessandro, Antonella D’Anneo, Francesco Pappalardo, Francesco Paolo Bonina, Maria Antonia Livrea, Mario Allegra, and Luisa Tesoriere. 2019. "Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro" Antioxidants 8, no. 10: 494. https://doi.org/10.3390/antiox8100494

APA Style

Attanzio, A., D’Anneo, A., Pappalardo, F., Bonina, F. P., Livrea, M. A., Allegra, M., & Tesoriere, L. (2019). Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro. Antioxidants, 8(10), 494. https://doi.org/10.3390/antiox8100494

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