Next Article in Journal
Separation and Identification of Highly Efficient Antioxidant Peptides from Eggshell Membrane
Next Article in Special Issue
Antioxidant and Anti-Inflammatory Properties of Plants Extract
Previous Article in Journal
In Vitro and In Vivo Antioxidant Activities of Polysaccharides Isolated from Celluclast-Assisted Extract of an Edible Brown Seaweed, Sargassum fulvellum
Previous Article in Special Issue
Glycerolic Licorice Extracts as Active Cosmeceutical Ingredients: Extraction Optimization, Chemical Characterization, and Biological Activity
Open AccessArticle

Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro

1
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, Università di Palermo, 90123 Palermo, Via Archirafi, Italy
2
Department Analytics of R&D, Bionap s.r.l., C.da Fureria, Zona Industriale Ovest, Piano Tavola, 95032 Belpasso (CT), Italy
3
Department of Pharmaceutical Science, Faculty of Pharmacy, Università di Catania, Viale Andrea Doria, 95125 Catania, Italy
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(10), 494; https://doi.org/10.3390/antiox8100494
Received: 4 October 2019 / Revised: 14 October 2019 / Accepted: 15 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Antioxidant and Anti-inflammatory Properties of Plants Extract)
Manna, a very singular vegetable product derived from the spontaneous solidification of the sap of some Fraxinus species, has long been known for its mild laxative and emollient properties. In this work, a hydro-alcoholic extract of manna (HME) from Sicilian Fraxinus angustifolia Vahl was investigated using HPLC-DAD to find phenol components and using chemical and biological in vitro assays to determine its reducing, antioxidant and anti-inflammatory capacity. We identified elenolic acid, tyrosol, hydroxytyrosol, catechin, fraxetin, verbascoside, gallic acid, procyanidin-B1, and luteolin 3,7 glucoside, in order of abundance. Measurements of total antioxidant activity by Folin-Ciocalteu reaction and ferric reducing ability (FRAP), as well as of scavenger activity towards ABTS•+, DPPH•, and perferryl-myoglobin radicals, showed that the phytocomplex effectively reduced oxidants with different standard potentials. When compared with vitamin E, HME also behaved as an efficient chain-breaking antioxidant against lipoperoxyl radicals from methyl linoleate. In cellular models for oxidative stress, HME counteracted membrane lipid oxidation of human erythrocytes stimulated by tert-butyl hydroperoxide and prevented the generation of reactive oxygen species, as well as the GSH decay in IL-1β–activated intestinal normal-like cells. Moreover, in this in vitro intestinal bowel disease model, HME reduced the release of the pro-inflammatory cytokines IL-6 and IL-8. These findings may suggest that manna acts as an antioxidant and anti-inflammatory natural product in humans, beyond its well-known effects against constipation. View Full-Text
Keywords: manna; Fraxinus; manna bioactivity; phytochemicals; antioxidant; reducing power; red blood cell oxidation; intestinal bowel disease model manna; Fraxinus; manna bioactivity; phytochemicals; antioxidant; reducing power; red blood cell oxidation; intestinal bowel disease model
Show Figures

Figure 1

MDPI and ACS Style

Attanzio, A.; D’Anneo, A.; Pappalardo, F.; Bonina, F.P.; Livrea, M.A.; Allegra, M.; Tesoriere, L. Phenolic Composition of Hydrophilic Extract of Manna from Sicilian Fraxinus angustifolia Vahl and its Reducing, Antioxidant and Anti-Inflammatory Activity in Vitro. Antioxidants 2019, 8, 494.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop