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Pathogens, Volume 12, Issue 8

August 2023 - 97 articles

Cover Story: Tick-borne pathogens (TBPs) pose a significant problem globally, particularly in tropical and subtropical regions, impacting both livestock industries and animal health. In Cuba, key pathogens affecting cattle include Babesia bovis, Babesia bigemina, and Anaplasma marginale. These pathogens are primarily transmitted by the Rhipicephalus microplus tick. Diagnosing these infections is challenging, due to low parasitemia levels during persistent infections and cross-reactivity. Molecular techniques such as PCR variants are essential for diagnosis. A drawback of PCR-based methods is their inability to simultaneously detect pathogens. However, high-throughput microfluidic methods enable for the concurrent detection of various pathogens. This study aims to investigate TBPs in cattle and ticks at four sampling points using real-time microfluidic PCR. View this paper
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Articles (97)

  • Article
  • Open Access
4 Citations
2,776 Views
11 Pages

Occurrence of Leishmania infantum in Wild Mammals Admitted to Recovery Centers in Spain

  • Iris Azami-Conesa,
  • Paula Pérez-Moreno,
  • Pablo Matas Méndez,
  • Jose Sansano-Maestre,
  • Fernando González,
  • Marta Mateo Barrientos and
  • María Teresa Gómez-Muñoz

16 August 2023

Zoonotic leishmaniasis caused by Leishmania infantum is distributed worldwide and affects humans and domestic and wild mammals. In Europe, specifically in the Mediterranean basin, leishmaniasis is endemic due to the concurrence of the phlebotomine ve...

  • Article
  • Open Access
5 Citations
3,019 Views
17 Pages

Timeline of SARS-CoV-2 Transmission in Sabah, Malaysia: Tracking the Molecular Evolution

  • Krishnan Nair Balakrishnan,
  • Chee Wei Yew,
  • Eric Tzyy Jiann Chong,
  • Sylvia Daim,
  • Nurul Elyani Mohamad,
  • Kenneth Rodrigues and
  • Ping-Chin Lee

15 August 2023

Background: The COVID-19 pandemic poses an unprecedented public health challenge in Malaysia. The impact of COVID-19 varies between countries, including geographically divided states within a country. The deadly transmission of COVID-19 has taken a h...

  • Article
  • Open Access
14 Citations
2,890 Views
11 Pages

First Molecular Evidence of Babesia vogeli, Babesia vulpes, and Theileria ovis in Dogs from Kyrgyzstan

  • Kursat Altay,
  • Ufuk Erol,
  • Omer Faruk Sahin,
  • Mehmet Fatih Aydin,
  • Ayperi Aytmirzakizi and
  • Nazir Dumanli

15 August 2023

Tick-borne parasitic diseases cause mild to severe infections among vertebrate hosts, including dogs. Species in the genus Babesia are important tick-borne pathogens and have worldwide distributions. Although there are data on the prevalence and dist...

  • Article
  • Open Access
3 Citations
4,040 Views
13 Pages

Molecular Detection and Identification of Piroplasm in Cattle from Kathmandu Valley, Nepal

  • Medhavi Dhakal,
  • Tulsi Ram Gompo,
  • Prakash Devkota,
  • Sharmila Chapagain Kafle,
  • Janak Raj Subedi,
  • Haiyan Gong,
  • Hiroaki Arima,
  • Richard Culleton,
  • Masahito Asada and
  • Kishor Pandey

15 August 2023

Background: Tick-borne protozoan parasites (TBPPs) cause significant problems for domestic animals’ health in Nepal. TBPPs are routinely diagnosed by labor-intensive blood smear microscopy. In Nepal, there are some reports of Babesia and Theile...

  • Article
  • Open Access
7 Citations
2,153 Views
13 Pages

Patterns, Cost, and Immunological Response of MDR vs. Non MDR-Bacteremia: A Prospective Cohort Study

  • Georgios Schinas,
  • Katerina Skintzi,
  • Anne-Lise De Lastic,
  • Maria Rodi,
  • Charalambos Gogos,
  • Athanasia Mouzaki and
  • Karolina Akinosoglou

15 August 2023

Background: Antimicrobial resistance (AMR) is a significant global health concern, posing a critical challenge for the effective management of infectious diseases. This study aimed to compare the immunological response, clinical outcomes, and associa...

  • Article
  • Open Access
5 Citations
3,076 Views
13 Pages

Presence of Equine and Bovine Coronaviruses, Endoparasites, and Bacteria in Fecal Samples of Horses with Colic

  • Moritz Stummer,
  • Vicky Frisch,
  • Frauke Glitz,
  • Barbara Hinney,
  • Joachim Spergser,
  • Jürgen Krücken,
  • Irina Diekmann,
  • Katharina Dimmel,
  • Christiane Riedel and
  • Jessika-Maximiliane V. Cavalleri
  • + 4 authors

15 August 2023

Acute abdominal pain (colic) is one of the major equine health threats worldwide and often necessitates intensive veterinary medical care and surgical intervention. Equine coronavirus (ECoV) infections can cause colic in horses but are rarely conside...

  • Article
  • Open Access
7 Citations
2,188 Views
15 Pages

14 August 2023

Background: Alveolar echinococcosis (AE) is a severe larval tapeworm infection with a variable clinical course of the disease. Reliable imaging techniques and biomarkers are needed to predict the course of the disease. Methods: 179 AE patients that r...

  • Article
  • Open Access
11 Citations
2,469 Views
14 Pages

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

  • Christina M. Lazov,
  • Alice Papetti,
  • Graham J. Belsham,
  • Anette Bøtner,
  • Thomas Bruun Rasmussen and
  • Maria Beatrice Boniotti

14 August 2023

It is important to be able to detect and differentiate between distinct porcine enteric coronaviruses that can cause similar diseases. However, the existence of naturally occurring recombinant coronaviruses such as swine enteric coronavirus (SeCoV) c...

  • Article
  • Open Access
1,682 Views
13 Pages

Macroelement and Microelement Levels in the Urine in Experimental Acanthamoebiasis

  • Natalia Łanocha-Arendarczyk,
  • Karolina Kot,
  • Irena Baranowska-Bosiacka,
  • Patrycja Kupnicka,
  • Dagmara Przydalska,
  • Aleksandra Łanocha,
  • Dariusz Chlubek,
  • Iwona Wojciechowska-Koszko and
  • Danuta Izabela Kosik-Bogacka

14 August 2023

Free-living amoebas can impact the excretion of macroelements and microelements in urine. The aim of the present study was to examine the concentrations of macroelements, including calcium (Ca), phosphorus (P), sodium (Na), potassium (K), and magnesi...

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Pathogens - ISSN 2076-0817