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Pathogens, Volume 12, Issue 8

August 2023 - 97 articles

Cover Story: Tick-borne pathogens (TBPs) pose a significant problem globally, particularly in tropical and subtropical regions, impacting both livestock industries and animal health. In Cuba, key pathogens affecting cattle include Babesia bovis, Babesia bigemina, and Anaplasma marginale. These pathogens are primarily transmitted by the Rhipicephalus microplus tick. Diagnosing these infections is challenging, due to low parasitemia levels during persistent infections and cross-reactivity. Molecular techniques such as PCR variants are essential for diagnosis. A drawback of PCR-based methods is their inability to simultaneously detect pathogens. However, high-throughput microfluidic methods enable for the concurrent detection of various pathogens. This study aims to investigate TBPs in cattle and ticks at four sampling points using real-time microfluidic PCR. View this paper
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Articles (97)

  • Article
  • Open Access
4 Citations
2,710 Views
13 Pages

Generation of Stable Cell Lines Expressing Akabane Virus N Protein and Insight into Its Function in Viral Replication

  • Jingjing Wang,
  • Dongjie Chen,
  • Fang Wei,
  • Junhua Deng,
  • Jia Su,
  • Xiangmei Lin and
  • Shaoqiang Wu

18 August 2023

Akabane virus (AKAV) is a world wide epidemic arbovirus belonging to the Bunyavirales order that predominantly infects livestock and causes severe congenital malformations. The nucleocapsid (N) protein of AKAV possesses multiple important functions i...

  • Article
  • Open Access
2 Citations
2,223 Views
13 Pages

Additive Effects of Cyclic Peptide [R4W4] When Added Alongside Azithromycin and Rifampicin against Mycobacterium avium Infection

  • Melissa Kelley,
  • Kayvan Sasaninia,
  • Arbi Abnousian,
  • Ali Badaoui,
  • James Owens,
  • Abrianna Beever,
  • Nala Kachour,
  • Rakesh Kumar Tiwari and
  • Vishwanath Venketaraman

18 August 2023

Mycobacterium avium (M. avium), a type of nontuberculous mycobacteria (NTM), poses a risk for pulmonary infections and disseminated infections in immunocompromised individuals. Conventional treatment consists of a 12-month regimen of the first-line a...

  • Article
  • Open Access
5 Citations
2,451 Views
15 Pages

18 August 2023

The abundant and widely distributed deermice Peromyscus leucopus and P. maniculatus are important reservoirs for several different zoonotic agents in North America. For the pathogens they persistently harbor, these species are also examples of the ph...

  • Article
  • Open Access
6 Citations
1,965 Views
9 Pages

Optimization and Validation of Reverse Transcription Recombinase-Aided Amplification (RT-RAA) for Sorghum Mosaic Virus Detection in Sugarcane

  • Fenglin Wang,
  • Qinmin Liang,
  • Rongman Lv,
  • Shakeel Ahmad,
  • Mishal Bano,
  • Guangzhen Weng and
  • Ronghui Wen

18 August 2023

Sorghum mosaic virus (SrMV) causes sugarcane mosaic disease and has significant adverse economic impacts on the cultivation of sugarcane. This study aimed to develop a rapid isotherm nucleic acid amplification method for detecting SrMV. Specific prim...

  • Case Report
  • Open Access
6 Citations
9,907 Views
11 Pages

Granulomatous Secondary Syphilis: A Case Report with a Brief Overview of the Diagnostic Role of Immunohistochemistry

  • Francesca Ambrogio,
  • Gerardo Cazzato,
  • Caterina Foti,
  • Mauro Grandolfo,
  • Gisella Biagina Mennuni,
  • Gino Antonio Vena,
  • Nicoletta Cassano,
  • Teresa Lettini,
  • Cosimo Castronovi and
  • Vito Ingordo
  • + 2 authors

18 August 2023

The diagnosis of syphilis can be challenging for dermatologists and dermatopathologists. In particular, secondary syphilis can have different clinical and histopathological presentations. A granulomatous tissue response is an unusual finding in secon...

  • Article
  • Open Access
7 Citations
4,395 Views
14 Pages

Biologically Relevant Murine Models of Chronic Pseudomonas aeruginosa Respiratory Infection

  • Aoife M. Rodgers,
  • Jaime Lindsay,
  • Avril Monahan,
  • Alice V. Dubois,
  • Aduragbemi A. Faniyi,
  • Barry J. Plant,
  • Marcus A. Mall,
  • Miquel B. Ekkelenkamp,
  • Stuart Elborn and
  • Rebecca J. Ingram

17 August 2023

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and the leading cause of infection in patients with cystic fibrosis (CF). The ability of P. aeruginosa to evade host responses and develop into chronic infection causes significant m...

  • Article
  • Open Access
1 Citations
2,502 Views
18 Pages

Using Antimicrobial Photodynamic Therapy with Ultrasound Devices and Bioactive Glasses as a Combined Approach for Treating Dentin Caries Lesions

  • João Felipe Besegato,
  • Priscila Borges Gobbo de Melo,
  • Adilson César Abreu Bernardi,
  • Marina Trevelin Souza,
  • Edgar Dutra Zanotto,
  • Vanderlei Salvador Bagnato and
  • Alessandra Nara de Souza Rastelli

17 August 2023

Novel approaches for caries lesion removal and treatment have been proposed. This study evaluates the combined use of an experimental ultrasound, aPDT (antimicrobial photodynamic therapy) and bioactive glasses on the removal, decontamination and remi...

  • Article
  • Open Access
1,953 Views
16 Pages

Naked Oat and Fusarium culmorum (W.G.Sm.) Sacc. Responses to Growth Regulator Effects

  • Sulukhan K. Temirbekova,
  • Oksana B. Polivanova,
  • Irina I. Sardarova,
  • Sholpan O. Bastaubaeva,
  • Elena A. Kalashnikova,
  • Marat Sh. Begeulov,
  • Mukhtar Zh. Ashirbekov,
  • Yuliya V. Afanasyeva,
  • Natalya S. Zhemchuzhina and
  • Natalya E. Ionova
  • + 3 authors

17 August 2023

The antioxidant defense system can be stimulated by growth regulators in plants when they are under stress, such as exposure to pathogens. There are a lot of natural growth regulators on the market, but no research has been carried out yet to determi...

  • Review
  • Open Access
38 Citations
4,889 Views
20 Pages

16 August 2023

Blastocystis is a common gut protist of humans and various animals worldwide, with a high level of genetic diversity. Neither its zoonotic potential and transmission routes nor its pathogenicity are fully known. This fact, and the fact that Blastocys...

  • Article
  • Open Access
2 Citations
1,992 Views
14 Pages

Taurine, a Component of the Tear Film, Exacerbates the Pathogenic Mechanisms of Acanthamoeba castellanii in the Ex Vivo Amoebic Keratitis Model

  • Lizbeth Salazar-Villatoro,
  • Bibiana Chávez-Munguía,
  • Celia Esther Guevara-Estrada,
  • Anel Lagunes-Guillén,
  • Dolores Hernández-Martínez,
  • Ismael Castelan-Ramírez and
  • Maritza Omaña-Molina

16 August 2023

Acanthamoeba spp. is the etiological agent of amoebic keratitis. In this study, the effect of taurine in physiological concentrations in tears (195 μM) on trophozoites of Acanthamoeba castellanii through the ex vivo amoebic keratitis model was eva...

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Pathogens - ISSN 2076-0817