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Volume 10
Issue 12
10.3390/genes10120951
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Article

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

by
Ning Wei
1,2,
Yongzhi Zhong
2,
Lulu Lin
2,
Minghui Xie
2,
Guangling Zhang
2,
Weihua Su
2,
Chuanren Li
1,* and
Haoliang Chen
2,*
1
College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China
2
Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(12), 951; https://doi.org/10.3390/genes10120951
Received: 20 September 2019 / Revised: 13 November 2019 / Accepted: 18 November 2019 / Published: 20 November 2019
(This article belongs to the Special Issue Arthropod Genetics and Genomics)
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Abstract

Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 60–2267 differentially expressed unigenes (DEUs). Among these DEUs, 31–790 unigenes were classified into 66–786 categories of gene ontology (GO) functional groups, and 24–760 DEUs could be mapped into 54–268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids. View Full-Text
Keywords: Sitobion avenae; RNA-seq; differentially expressed unigenes; insecticide tolerance-related genes Sitobion avenae; RNA-seq; differentially expressed unigenes; insecticide tolerance-related genes
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MDPI and ACS Style

Wei, N.; Zhong, Y.; Lin, L.; Xie, M.; Zhang, G.; Su, W.; Li, C.; Chen, H. Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae. Genes 2019, 10, 951. https://doi.org/10.3390/genes10120951

AMA Style

Wei N, Zhong Y, Lin L, Xie M, Zhang G, Su W, Li C, Chen H. Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae. Genes. 2019; 10(12):951. https://doi.org/10.3390/genes10120951

Chicago/Turabian Style

Wei, Ning, Yongzhi Zhong, Lulu Lin, Minghui Xie, Guangling Zhang, Weihua Su, Chuanren Li, and Haoliang Chen. 2019. "Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae" Genes 10, no. 12: 951. https://doi.org/10.3390/genes10120951

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