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Open AccessArticle

Evaluation of Lipid Accumulation Using Electrical Impedance Measurement under Three-Dimensional Culture Condition

by Daiki Zemmyo 1 and Shogo Miyata 2,*
1
Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan
2
Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(7), 455; https://doi.org/10.3390/mi10070455
Received: 17 May 2019 / Revised: 26 June 2019 / Accepted: 2 July 2019 / Published: 6 July 2019
The degeneration of adipocyte has been reported to cause obesity, metabolic syndrome, and other diseases. To treat these diseases, an effective in vitro evaluation and drug-screening system for adipocyte culture is required. The objective of this study is to establish an in vitro three-dimensional cell culture system to enable the monitoring of lipid accumulation by measuring electrical impedance, and to determine the relationship between the impedance and lipid accumulation of adipocytes cultured three dimensionally. Consequently, pre-adipocytes, 3T3-L1 cells, were cultured and differentiated to the adipocytes in our culture system, and the electrical impedance of the three-dimensional adipocyte culture at a high frequency was related to the lipid accumulation of the adipocytes. In conclusion, the lipid accumulation of adipocytes could be evaluated in real time by monitoring the electrical impedance during in vitro culture. View Full-Text
Keywords: electrical impedance measurement; three-dimensional cell culture; adipocyte; lipid droplet; 3T3-L1 electrical impedance measurement; three-dimensional cell culture; adipocyte; lipid droplet; 3T3-L1
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Zemmyo, D.; Miyata, S. Evaluation of Lipid Accumulation Using Electrical Impedance Measurement under Three-Dimensional Culture Condition. Micromachines 2019, 10, 455.

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