Next Article in Journal
Association between Vitamin D Deficiency and Single Nucleotide Polymorphisms in the Vitamin D Receptor and GC Genes and Analysis of Their Distribution in Mexican Postmenopausal Women
Next Article in Special Issue
Effect of Moringa oleifera Leaf Powder on Postprandial Blood Glucose Response: In Vivo Study on Saharawi People Living in Refugee Camps
Previous Article in Journal
Impact of Inflammation on Ferritin, Hepcidin and the Management of Iron Deficiency Anemia in Chronic Kidney Disease
Previous Article in Special Issue
Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells
Open AccessArticle

Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3)

1
UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
2
Department of Biochemistry, Kaduna State University, 2336 Kaduna, Nigeria
3
Department of Biology, Faculty of Science, El-Mergib University, El Khums, Libya
4
Laboratory of Biomolecular Medicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
5
Department of Biochemistry and Forensic Science, Nigeria Police Academy, Wudil, 3474 Kano, Nigeria
6
The Mitomasa Sdn. Bhd., 13-1, Jalan L J 3, Taman Industri Lembah Jaya, 68000 Ampang, Selangor, Malaysia
7
Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
8
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(9), 1174; https://doi.org/10.3390/nu10091174
Received: 12 July 2018 / Revised: 14 August 2018 / Accepted: 17 August 2018 / Published: 27 August 2018
(This article belongs to the Special Issue Plant Food, Nutrition and Human Health)
The incidence of prostate cancer malignancy along with other cancer types is increasing worldwide, resulting in high mortality rate due to lack of effective medications. Moringa oleifera has been used for the treatment of communicable and non-communicable ailments across tropical countries, yet, little has been documented regarding its effect on prostate cancer. We evaluated the acute toxicity and apoptosis inducing effect of glucomoringin-isothiocyanate rich soluble extracts (GMG-ITC-RSE) from M. oleifera in vivo and in vitro, respectively. Glucomoringin was isolated, identified, and characterized using fundamental analytical chemistry tools where Sprague-Dawley (SD) rats, murine fibroblast (3T3), and human prostate adenocarcinoma cells (PC-3) were used for acute toxicity and bioassays experiments. GMG-ITC-RSE did not instigate adverse toxic reactions to the animals even at high doses (2000 mg/kg body weight) and affected none of the vital organs in the rats. The extract exhibited high levels of safety in 3T3 cells, where more than 90% of the cells appeared viable when treated with the extract in a time-dependent manner even at high dose (250 µg/mL). GMG-ITC-RSE significantly triggered morphological aberrations distinctive to apoptosis observed under microscope. These findings obviously revealed the putative safety of GMG-ITC-RSE in vivo and in vitro, in addition to its anti-proliferative effect on PC-3 cells. View Full-Text
Keywords: acute toxicity; anti-proliferation; apoptosis; cytotoxicity; glucomoringin-isothiocyanate; PC-3 acute toxicity; anti-proliferation; apoptosis; cytotoxicity; glucomoringin-isothiocyanate; PC-3
Show Figures

Figure 1

MDPI and ACS Style

Jaafaru, M.S.; Abd Karim, N.A.; Mohamed Eliaser, E.; Maitalata Waziri, P.; Ahmed, H.; Mustapha Barau, M.; Kong, L.; Abdull Razis, A.F. Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3). Nutrients 2018, 10, 1174.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop