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Viruses, Volume 13, Issue 4

April 2021 - 183 articles

Cover Story: Volume electron microscopy (vEM) refers to a group of technologies that enable the efficient ultrastructural imaging of µm3–mm3 volumes of biospecimens in 3-D and at nanoscale resolutions. Of these, focused ion beam scanning electron microscopy (FIB-SEM) holds great potential for researchers interested in interrogating virus–cell interactions in 3-D, provided they can evaluate its strengths and limitations. To this end, we have written a brief primer on FIB-SEM in the context of vEM approaches. Aimed at the virologist or cell biologist, we use the example of 3-D visualization of SARS-CoV-2 virus–cell interactions to illustrate practical aspects of an FIB-SEM experiment. View this paper
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Articles (183)

  • Review
  • Open Access
27 Citations
6,212 Views
27 Pages

20 April 2021

Rift Valley fever phlebovirus (RVFV) infects humans and a wide range of ungulates and historically has caused devastating epidemics in Africa and the Arabian Peninsula. Lesions of naturally infected cases of Rift Valley fever (RVF) have only been des...

  • Article
  • Open Access
2 Citations
2,851 Views
11 Pages

Hematological Profile of Pregnant Women with Suspected Zika Virus Infection Followed Up at a Referral Service in Manaus, Brazil

  • Anny Beatriz Costa Antony de Andrade,
  • Maria Jacirema Ferreira Gonçalves,
  • Elijane de Fátima Redivo,
  • Maria das Graças Costa Alecrim and
  • Flor Ernestina Martinez-Espinosa

20 April 2021

The purpose of this paper is to describe the hematological profile of pregnant women with suspected Zika virus (ZIKV) infection followed up at a reference service for infectious diseases in Manaus, Brazil, through a clinical, epidemiological, cross-s...

  • Article
  • Open Access
6 Citations
3,033 Views
10 Pages

Persistence of Anti-ZIKV-IgG over Time Is Not a Useful Congenital Infection Marker in Infants Born to ZIKV-Infected Mothers: The NATZIG Cohort

  • Conrado Milani Coutinho,
  • Juliana Dias Crivelenti Pereira Fernandes,
  • Aparecida Yulie Yamamoto,
  • Silvia Fabiana Biason de Moura Negrini,
  • Bento Vidal de Moura Negrini,
  • Sara Reis Teixeira,
  • Fabiana Rezende Amaral,
  • Márcia Soares Freitas da Motta,
  • Adriana Aparecida Tiraboschi Bárbaro and
  • Davi Casale Aragon
  • + 5 authors

20 April 2021

Confirming ZIKV congenital infection is challenging because viral RNA is infrequently detected. We compared the presence of anti-ZIKV-IgM and the persistence of anti-ZIKV-IgG antibodies over 18 months in two cohorts of infants born to ZIKV-infected m...

  • Article
  • Open Access
9 Citations
3,728 Views
14 Pages

20 April 2021

Dengue virus (DENV) infection, which causes dengue fever, dengue hemorrhagic fever, and dengue shock syndrome, is a severe global health problem in tropical and subtropical areas. There is no effective vaccine or drug against DENV infection. Thus, th...

  • Article
  • Open Access
26 Citations
5,201 Views
15 Pages

Comparison of Serological Assays for the Detection of SARS-CoV-2 Antibodies

  • Joe James,
  • Shelley Rhodes,
  • Craig S. Ross,
  • Paul Skinner,
  • Samuel P. Smith,
  • Rebecca Shipley,
  • Caroline J. Warren,
  • Hooman Goharriz,
  • Lorraine M. McElhinney and
  • Nigel Temperton
  • + 6 authors

20 April 2021

SARS-CoV-2 virus was first detected in late 2019 and circulated globally, causing COVID-19, which is characterised by sub-clinical to severe disease in humans. Here, we investigate the serological antibody responses to SARS-CoV-2 infection during acu...

  • Article
  • Open Access
4 Citations
3,592 Views
18 Pages

Impact of IL10, MTP, SOD2, and APOE Gene Polymorphisms on the Severity of Liver Fibrosis Induced by HCV Genotype 4

  • Amr Ali Hemeda,
  • Amal Ahmad Mohamed,
  • Ramy Karam Aziz,
  • Mohamed S. Abdel-Hakeem and
  • Marwa Ali-Tammam

20 April 2021

Complications of hepatitis C virus (HCV) chronic infection cause ~400,000 deaths worldwide annually. One complication, liver fibrosis, is influenced by host genetic factors. Genes influencing fibrosis include immune, metabolic, oxidative stress, and...

  • Article
  • Open Access
11 Citations
3,364 Views
12 Pages

A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen® or Taqman® Technologies

  • Fábio A. Abade dos Santos,
  • Carina L. Carvalho,
  • Maria C. Peleteiro,
  • Francisco Parra and
  • Margarida D. Duarte

20 April 2021

In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Th...

  • Review
  • Open Access
26 Citations
9,063 Views
17 Pages

The Nuclear Pore Complex Is a Key Target of Viral Proteases to Promote Viral Replication

  • Luis Adrián De Jesús-González,
  • Selvin Palacios-Rápalo,
  • José Manuel Reyes-Ruiz,
  • Juan Fidel Osuna-Ramos,
  • Carlos Daniel Cordero-Rivera,
  • Carlos Noé Farfan-Morales,
  • Ana Lorena Gutiérrez-Escolano and
  • Rosa María del Ángel

19 April 2021

Various viruses alter nuclear pore complex (NPC) integrity to access the nuclear content favoring their replication. Alteration of the nuclear pore complex has been observed not only in viruses that replicate in the nucleus but also in viruses with a...

  • Case Report
  • Open Access
18 Citations
4,802 Views
12 Pages

Detection of Laryngotracheitis Virus in Poultry Flocks with Respiratory Disorders in Slovenia

  • Olga Zorman Rojs,
  • Alenka Dovč,
  • Uroš Krapež,
  • Zoran Žlabravec,
  • Joško Račnik and
  • Brigita Slavec

19 April 2021

Infectious laryngotracheitis (ILT) is an acute, highly contagious infectious disease of the upper respiratory tract in chickens and other poultry species that causes significant economic losses in countries worldwide. Between 2017 and 2019, seven out...

  • Article
  • Open Access
12 Citations
6,512 Views
17 Pages

Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE

  • Su Datt Lam,
  • Paul Ashford,
  • Sandra Díaz-Sánchez,
  • Margarita Villar,
  • Christian Gortázar,
  • José de la Fuente and
  • Christine Orengo

19 April 2021

Coronavirus-like organisms have been previously identified in Arthropod ectoparasites (such as ticks and unfed cat flea). Yet, the question regarding the possible role of these arthropods as SARS-CoV-2 passive/biological transmission vectors is still...

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Viruses - ISSN 1999-4915