Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Viruses
2.2. Construction of the NS Plasmids
2.3. Rescue of Recombinant Reporter-Expressing ∆NS1 Viruses and Viral Infections
2.4. Virus Growth Kinetics
2.5. Protein Gel Electrophoresis and Western Blot Analysis
2.6. Fluorescence and Indirect Immunofluorescence Assays
2.7. Plaque Assays
2.8. Cell-Based IFN Bioassay
2.9. Mouse Studies
3. Results
3.1. Generation of a Recombinant ΔNS1 PR8 Virus Expressing mCherry
3.2. In Vitro Characterization of ΔNS1 mCherry
3.3. Ability of ΔNS1 mCherry to Inhibit IFNβ Promoter Activation
3.4. ΔNS1 mCherry Infection in Mice
3.5. Generation and Characterization of a Replication-Competent ΔNS1 Nluc
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nogales, A.; Schotsaert, M.; Rathnasinghe, R.; DeDiego, M.L.; García-Sastre, A.; Martinez-Sobrido, L. Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes. Viruses 2021, 13, 698. https://doi.org/10.3390/v13040698
Nogales A, Schotsaert M, Rathnasinghe R, DeDiego ML, García-Sastre A, Martinez-Sobrido L. Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes. Viruses. 2021; 13(4):698. https://doi.org/10.3390/v13040698
Chicago/Turabian StyleNogales, Aitor, Michael Schotsaert, Raveen Rathnasinghe, Marta L. DeDiego, Adolfo García-Sastre, and Luis Martinez-Sobrido. 2021. "Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes" Viruses 13, no. 4: 698. https://doi.org/10.3390/v13040698
APA StyleNogales, A., Schotsaert, M., Rathnasinghe, R., DeDiego, M. L., García-Sastre, A., & Martinez-Sobrido, L. (2021). Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes. Viruses, 13(4), 698. https://doi.org/10.3390/v13040698