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Viruses, Volume 12, Issue 11

November 2020 - 142 articles

Cover Story: HIV-1 cure strategy by means of proviral knock-out using CRISPR-Cas9 has been hampered by the emergence of viral resistance against the targeting guide RNA (gRNA). Rapid viral rebound was evident after Cas9-gRNA disruption, due to the non-homologous end-joining (NHEJ)-mediated mutations inside the targeted proviral regions (e.g., LTR) that are non-deleterious to the virus. Here, we propose concentrated gRNA attacks against the integral HIV-1 tat to deplete post-editing viral fitness and block mutational escape. Analogous to the combination strategy of antiretroviral therapy (ART), combination of multiple gRNAs represents a promising method to inhibit viral escape from CRISPR-Cas9, thus elevating the gene editing platform to achieve long-term HIV-1 cure. View this paper.
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Articles (142)

  • Review
  • Open Access
41 Citations
10,848 Views
16 Pages

Plant Virology Delivers Diverse Toolsets for Biotechnology

  • Mo Wang,
  • Shilei Gao,
  • Wenzhi Zeng,
  • Yongqing Yang,
  • Junfei Ma and
  • Ying Wang

23 November 2020

Over a hundred years of research on plant viruses has led to a detailed understanding of viral replication, movement, and host–virus interactions. The functions of vast viral genes have also been annotated. With an increased understanding of pl...

  • Article
  • Open Access
41 Citations
5,704 Views
9 Pages

23 November 2020

Multidrug-resistant (MDR) bacteria are a major threat to public health. Bacteriophage endolysins (lysins) are a promising alternative treatment to traditional antibiotics. However, the lysins currently under development are still underestimated. Here...

  • Opinion
  • Open Access
27 Citations
4,561 Views
10 Pages

A Perspective on Organoids for Virology Research

  • Adithya Sridhar,
  • Salvatore Simmini,
  • Carla M. S. Ribeiro,
  • Caroline Tapparel,
  • Melvin M. Evers,
  • Dasja Pajkrt and
  • Katja Wolthers

23 November 2020

Animal models and cell lines are invaluable for virology research and host–pathogen interaction studies. However, it is increasingly evident that these models are not sufficient to fully understand human viral diseases. With the advent of three...

  • Article
  • Open Access
12 Citations
3,748 Views
21 Pages

Examining the Association of Socioeconomic Position with Microcephaly and Delayed Childhood Neurodevelopment among Children with Prenatal Zika Virus Exposure

  • Grace M. Power,
  • Suzanna C. Francis,
  • Nuria Sanchez Clemente,
  • Zilton Vasconcelos,
  • Patricia Brasil,
  • Karin Nielsen-Saines,
  • Elizabeth B. Brickley and
  • Maria E. Moreira

23 November 2020

Increased rates of Zika virus have been identified in economically deprived areas in Brazil at the population level; yet, the implications of the interaction between socioeconomic position and prenatal Zika virus exposure on adverse neurodevelopmenta...

  • Article
  • Open Access
6 Citations
3,808 Views
18 Pages

High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus

  • Nildimar Alves Honório,
  • Daniel Cardoso Portela Câmara,
  • Keenan Wiggins,
  • Bradley Eastmond and
  • Barry Wilmer Alto

23 November 2020

Vector competence refers to the ability of a vector to acquire, maintain, and transmit a pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for approximating arbovirus transmission. However, this method is t...

  • Article
  • Open Access
16 Citations
3,912 Views
15 Pages

23 November 2020

Several viral factors impact the natural course of hepatitis B virus (HBV) infection, the sensitivity of diagnostic tests, or treatment response to interferon-α and nucleos(t)ide analogues. These factors include the viral genotype and serotype...

  • Review
  • Open Access
43 Citations
8,314 Views
20 Pages

Host Range and Coding Potential of Eukaryotic Giant Viruses

  • Tsu-Wang Sun,
  • Chia-Ling Yang,
  • Tzu-Tong Kao,
  • Tzu-Haw Wang,
  • Ming-Wei Lai and
  • Chuan Ku

21 November 2020

Giant viruses are a group of eukaryotic double-stranded DNA viruses with large virion and genome size that challenged the traditional view of virus. Newly isolated strains and sequenced genomes in the last two decades have substantially advanced our...

  • Article
  • Open Access
18 Citations
6,710 Views
17 Pages

20 November 2020

Sea star wasting disease (SSWD) is a condition that has affected asteroids for over 120 years, yet mechanistic understanding of this wasting etiology remains elusive. We investigated temporal virome variation in two Pisaster ochraceus specimens that...

  • Review
  • Open Access
27 Citations
6,170 Views
22 Pages

20 November 2020

Chronic hepatitis D (CHD) is the most severe form of viral hepatitis, with rapid progression of liver-related diseases and high rates of development of hepatocellular carcinoma. The causative agent, hepatitis D virus (HDV), contains a small (approxim...

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Viruses - ISSN 1999-4915