Plant Species Effect on Soil Micronutrients and Aluminum in Secondary Forests at Masako Forest Reserve, Kisangani, Democratic Republic of Congo

Plant species can significantly influence soil micronutrients. We assessed how soil micronutrients (B, Fe, Cu, Zn, Mn) and aluminum (Al) were affected by soil depth (SD) and plant species (PS) in a secondary forest at Masako Forest Reserve. Soil samples were collected in June 2022 and June 2023 along five PS (Entandrophragma utile, Hevea brasiliensis, Milettia laurentii, Musanga cecropoides, and Triculia africana). Four trees (replications) were selected per plant species. A completely randomized design was used with five PS and three SD (0–10 cm, 10–20, and 20–30 cm) and was replicated four times. To collect soil samples, a pit was dug at each sampling location (near a tree), and three soil samples were taken horizontally in the middle of each layer on one of the four faces of the pit, with a 5 cm height and 5 cm diameter cylinder. Soil samples were air-dried, mixed, and sieved to 2 mm, and a 20 g subsample was sent to Brookside Laboratories (OH, USA) for analyses of soil micronutrients. The results showed that most micronutrients were concentrated in the topsoil (0–10 cm). Plant species such as Treculia africana, Millettia laurentii, and Musanga cecropoides enhanced micronutrients in the soil in which they grew, especially iron (Fe) and zinc (Zn). The effect of the year of sampling on micronutrients was prevalent for many micronutrients, which remained significantly higher in 2022 than in 2023. These findings provide a foundational framework for developing nature-based biofortification strategies. By prioritizing key native plant species, local stakeholders can optimize soil health in the Congo Basin.