Search Results (28,686)

As aquafeed formulations include more plant-based materials, mycotoxin contamination is becoming relevant for fish farming. Deoxynivalenol (DON) and fumonisin B1 (FB1) frequently occur in fish feeds and may impair fish performance and health. This study evaluated the impact of DON and FB1 on growth and health parameters in gilthead seabream (Sparus aurata). Fish were fed ad libitum for seven weeks with six diets assessed in triplicate: DON A (300 ppb), DON B (2000 ppb), DON C (5000 ppb), FB1 A (5 ppb), FB1 B (10 ppb), FB1 C (40 ppb). The highest contamination levels significantly reduced feed intake, body weight, total length, and biomass, while body weight was also reduced in the lowest FB1 treatment. The feed conversion ratio increased in exposed fish, whereas the specific growth rate decreased in DON B, DON C, FB1 A, and FB1 C. Both mycotoxins altered hematological profiles, immune-related enzymatic activities, and liver morphology. DNA damage increased with exposure level and was greatest at the highest DON concentration. Overall, this study shows that short-term dietary exposure can impair seabream performance and health, supporting stricter mycotoxin monitoring in fish feeds and research on chronic exposure, recovery, and species-specific safety thresholds. Full article

Elemental mercury (Hg⁰) removal in coal combustion flue-gas over commercial V-based SCR catalysts is still limited by insufficient low-temperature activity and the complex interference of gas components. In this work, V₂O₅/TiO₂, V₂O₅⁻MoO₃/TiO₂, and V₂O₅⁻MoS₂/TiO₂ catalysts were comparatively investigated for Hg⁰ removal under simulated coal combustion flue-gas conditions. Among them, V₂O₅⁻MoS₂/TiO₂ exhibited the best performance over the whole temperature window of 200–400 °C, reaching a Hg⁰ removal efficiency of 73.3% at 200 °C, which was markedly higher than those of V₂O₅/TiO₂ and V₂O₅⁻MoO₃/TiO₂. Under multicomponent SCR atmospheres in coal-fired plants, the catalyst also showed the highest Hg⁰ oxidation efficiency of 72.9%, indicating that MoS₂ modification was more effective than oxide promotion in enhancing low-temperature mercury removal. XRD and FT-IR results showed that MoS₂ and vanadia were successfully incorporated onto TiO₂ as dispersed surface phases, while the MoS₂-modified catalyst exhibited a distinct and persistent terminal V=O feature, implying the formation of a coupled Mo-S-V interfacial environment. H₂-TPR and NH₃-TPD demonstrated that MoS₂ modification simultaneously stabilized the redox structure and moderated the surface acidity, suppressing excessively strong NH₃ retention while maintaining a tunable oxidation-active surface. XPS analysis further revealed atmosphere-dependent redistribution of O_α/O_β species, sulfur oxidation to SO₃²⁻/SO₄²⁻ species, and dynamic V⁵⁺/V⁴⁺ interconversion, confirming that MoS₂ acted not only as a sulfur-containing component but also as an interfacial electronic regulator. Post-reaction Hg 4f XPS showed that retained mercury mainly existed as Hg²⁺ species, while Hg-TPD indicated that MoS₂ modification provided a more diverse and thermally stable mercury-binding environment. These results demonstrate that Hg⁰ removal over V₂O₅⁻MoS₂/TiO₂ proceeds through oxidation-retention coupling rather than simple oxidation alone. The enhanced performance originates from the synergistic effects of active oxygen migration, vanadium redox cycling, sulfur-assisted stabilization, and interfacial Mo-S-V electronic coupling. This work provides a promising strategy for designing multifunctional SCR catalysts in coal-fired plants for efficient Hg⁰ control under practical coal combustion flue-gas conditions. Full article

The rapid expansion of global aquaculture and increasing demand for sustainable protein sources have intensified the search for alternatives to conventional fish meal. Wolffia globosa, the smallest and fastest-growing flowering plant, has emerged as a promising yet underutilized aquatic resource with significant potential in aquaculture nutrition and sustainable food systems. Despite growing interest in aquatic plant-based proteins, comprehensive evaluation of W. globosa across nutrition, cultivation, and application systems remains limited. This review synthesizes current knowledge on its biology, nutritional composition, functional properties, and application potential in aquaculture and circular bioeconomy systems. W. globosa exhibits rapid vegetative propagation, high biomass productivity, and minimal resource requirements, enabling efficient cultivation in nutrient-rich environments. It contains high-quality protein (20–48% dry weight), essential amino acids, polyunsaturated fatty acids, dietary fiber, vitamins, minerals, and diverse bioactive compounds with antioxidant and immunomodulatory properties. Feeding studies demonstrate its potential to enhance growth performance, feed efficiency, and physiological health in aquatic species. Additionally, its capacity to absorb nitrogen and phosphorus supports nutrient recycling and water quality improvement in integrated aquaculture systems. However, variability in nutrient composition, limited large-scale cultivation data, insufficient long-term feeding trials, and uncertain economic feasibility at industrial scale remain key challenges for its commercial adoption. Overall, W. globosa represents a promising but still developing biomass resource with strong potential to reduce dependence on fish meal while supporting environmentally efficient aquaculture and future food systems. Future research should focus on optimizing cultivation practices, developing efficient processing technologies, and validating long-term feeding performance to facilitate its large-scale adoption. Full article

Low light is a major environmental constraint that limits crop productivity by restricting photosynthetic carbon assimilation and disrupting photosynthetic function. Nitric oxide (NO) is an important signaling molecule involved in plant stress responses; however, its role in regulating photosystem stability under low-light conditions remains unclear. In this study, tomato seedlings were treated with the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) to investigate the effects of NO-related treatments on growth, photosynthetic performance, photosystem function, electron transport, ROS accumulation, and antioxidant defense under low-light conditions. Low light markedly suppressed growth and impaired the photochemical activities of both photosystem II (PSII) and photosystem I (PSI), as indicated by decreases in the maximum quantum efficiency of PSII (Fv/Fm), the maximal P700 oxidation capacity (Pm), and electron transport rates, together with enhanced reactive oxygen species (ROS) accumulation. Exogenous NO application significantly improved photosystem performance under low-light conditions. NO enhanced PSII and PSI photochemical performance, improved excitation energy distribution, and restored photosynthetic electron transport, while strengthening antioxidant enzyme activities, thereby reducing excitation pressure and oxidative damage. In contrast, PTIO treatment aggravated photosystem impairment under low light. These findings indicate that NO contributes to maintaining photosystem function and photosynthetic electron transport under low-light conditions, highlighting its potential role in improving low-light tolerance in tomato. Full article

Insect herbivory plays a critical role in shaping plant growth, fitness, and population dynamics, yet its effects across African ecosystems remain poorly understood due to fragmented, methodologically diverse, and geographically biassed research. To address this gap, we conducted an integrated bibliometric and systematic review of peer-reviewed studies on insect herbivory and plant performance in Africa. Searches of Web of Science and Scopus identified 1642 records, of which 312 met relevance criteria and 157 satisfied quality thresholds, including clear herbivory metrics and robust experimental or observational designs. Bibliometric analyses revealed modest but increasing publication rates, with research concentrated in Southern and Eastern Africa and primarily focused on savannas and agroecosystems. Montane forests, wetlands, and arid ecosystems remain underrepresented. Most studies examined Lepidopteran leaf herbivory, while fewer than 15% addressed root, stem, or seed damage, and research collaborations were largely regional. Systematic synthesis showed that insect herbivory generally reduces seedling survival, height, and biomass, particularly at early life stages, although some species display tolerance or compensatory growth depending on environmental conditions and plant traits. Reported effect sizes were highly variable (Hedges’ g = 2.1–3.4), reflecting differences in ecological context, herbivore guilds, and study design. Few studies incorporated multiple life stages, trophic interactions, or climate gradients, limiting inference on long-term population and ecosystem responses. We emphasise the need for harmonised methodologies, trait-based and guild-specific approaches, long-term manipulative studies, and expanded research across underrepresented ecosystems to better inform conservation and restoration under environmental change. Full article

Pyracantha koidzumii (Hayata) Rehder is native to Taiwan, where it has an endangered IUCN conservation status, although it is invasive in other parts of the world. It was introduced to South Africa as an ornamental species. In South Africa, P. koidzumii is classified as having density-based negative impacts, although its distribution is sparse and no impact assessments have been conducted to date, compared with its established congener, Pyracantha angustifolia. We asked the following questions: (1) does the current distribution of P. koidzumii and P. angustifolia indicate any relationship with anthropogenic activities as the main driver of plant invasions; (2) what are the seed viability and germination capacity of P. koidzumii?; and (3) what is the current distribution and habitat suitability of P. koidzumii relative to P. angustifolia, and does P. koidzumii show any potential to be invasive in different climate change scenarios (i.e., SSP1 and SSP5). We found a significant relationship between land use and cover and species occurrence records, with disturbed areas having the highest occurrence records (43.5%; N = 4297), followed by grassland (23.4%). The seed viability and germination capacity were high (78.6–87.2%; n = 50), confirming that P. koidzumii can successfully recruit in southern Africa. Climate suitability projections across scenarios for both species yielded a strong data fit (i.e., training AUCs above 0.9), with precipitation as the most important climate predictor. The climate-based habitat suitability projections showed greater net expansion for both species in SSP1, with P. angustifolia projected to invade 254,974 km², while P. koidzumii could invade 49,829 km². Alternatively, there was contraction (i.e., positive net habitat loss) of suitable habitat in SSP5, characterised by significant area loss for P. angustifolia (12,0139 km²), while P. koidzumii displayed a moderate loss (13,174 km²). For the first time, this study showed that P. koidzumii has high potential to invade the southern African grassland biome, driven by high germination combined with biophysical disturbance, and that its future habitat occupancy will be moderately affected by extreme environmental temperature conditions. Full article

The widespread occurrence of microplastics (MPs) and nanoplastics (NPs) in wastewater treatment plants (WWTPs) has raised notable ecological concerns. This study investigated the multi-level impacts of polystyrene (PS) MPs and NPs on activated sludge in sequencing batch reactors (SBRs). Although long-term exposure to PS particles (up to 200 mg/L MPs and 50 mg/L NPs) did not compromise the macro-performance of the SBRs (with COD, NH₄⁺-N, and TP removal efficiencies remaining >90%), the physical and biological integrity of the sludge was significantly altered. Sludge volume index (SVI) analysis revealed a decline in settleability, particularly under NP stress, which was corroborated by laser scanning confocal microscopy (LSCM) and transmission electron microscopy (TEM) observations showing the internalization of NPs into microbial cells. Physiologically, PS NPs induced higher levels of reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release, indicating substantial oxidative stress and membrane damage. Furthermore, high-throughput sequencing and qPCR analysis demonstrated that PS particles reshaped the microbial community, enriching the antibiotic-resistant genus Acinetobacter and various antibiotic resistance genes (ARGs) such as sulII and tetG. Notably, PS NPs exhibited a more pronounced effect on ARG dissemination than MPs, even at lower mass concentrations, by facilitating horizontal gene transfer (HGT) through increased cell membrane permeability. These findings elucidate the distinct toxicological mechanisms of NPs in biological treatment systems and highlight their role as catalysts for the environmental spread of antibiotic resistance. Full article

Pathogenesis-related (PR) proteins are central to plant defense; however, their roles in regulating secondary metabolism remain largely unexplored. In Panax ginseng, the molecular characteristics and functional links of PR1 and PR10 gene families to ginsenoside biosynthesis remain unclear. Here, we conducted genome and transcriptome identification, evolutionary analysis, co-expression network construction, and methyl jasmonate (MeJA) induction assays to investigate their roles. We identified 34 PR1/PR10 family members (11 PgPR1s and 23 PgPR10s) unevenly distributed across 15 chromosomes, with gene duplication driving family expansion. Functional divergence was evident: PgPR1 members are evolutionarily conserved and root-preferential, associated with antibacterial defense, whereas PgPR10 underwent species-specific expansion, showed broad tissue expression, and possessed promoters enriched with hormone- and stress-responsive cis-elements. Co-expression analysis (|r| ≥ 0.3, p < 0.001) identified PgPR1-5 and PgPR10-8 as the only PR members significantly correlated with key ginsenoside biosynthetic enzymes. Under MeJA, the ginsenoside pathway displayed bidirectional temporal regulation, with core PR genes showing synchronized expression with the negatively regulated enzyme gene CAS_14. Our study characterizes the molecular and evolutionary features of PgPR1/PgPR10 families, identifies core PR genes associated with ginsenoside metabolism, and reveals their coordinated response to MeJA, providing key insights and candidate targets for exploring the potential link between PR proteins and ginsenoside biosynthesis and promising candidate genes for further research on P. ginseng. Full article

A circular DNA enrichment sequencing (CIDER-Seq) approach identified a New World (NW) begomovirus–alphasatellite complex associated with a leaf curl disease of tomato from plants collected in Morelos, México. Using one of 21 samples showing virosis-associated symptoms, CIDER-Seq data revealed the presence of various NW begomovirus species, namely Begomovirus solanumaureivariati (tomato golden mottle virus: ToGMoV), Begomovirus solanumseveri (tomato severe leaf curl virus: ToSLCV), and Begomovirus solanumlapazense (tomato chino La Paz virus: ToChLPV). Additionally, the alphasatellites Whiflysatellite guatemalaense (whitefly-associated Guatemala alphasatellite 1: WfaGA1) and Clecrusatellite guatemalaense (whitefly-associated Guatemala alphasatellite 2: WfaGA2) were identified, demonstrating that begomoviruses can co-infect with alphasatellites; however, the pathogenic implications of the alphasatellite–helper begomovirus association remain incompletely understood. PCR-based detection and Sanger sequencing of circular viral genomes revealed a high frequency of detection of mixed infections comprising all components of the begomovirus–alphasatellite complex, confirming the reliability of the CIDER-Seq approach in producing high-fidelity sequences. Molecular and biological analysis revealed the complex adaptation to tomato, the monopartite nature of ToSLCV/ToChLPV species, and the ability of these begomovirus species to act as helper viruses. To the best of our knowledge, this is the first report of leaf curl disease of tomato associated with a begomovirus–alphasatellite complex in Mexico. Full article

Plant functional traits are key indicators of plant responses to environmental conditions and resource-allocation trade-offs, thereby providing insights into adaptive mechanisms across habitats. Wetlands are one of the major habitats worldwide, yet organ-level plant life adaptive strategies in different wetland types remain insufficiently understood. This study compared three representative marsh systems located in different regions of Northeast China. Forty-five plant community plots were established, and 10 leaf traits, 13 root traits, and 8 soil environmental variables were systematically measured for dominant herbaceous species. We examined above- and belowground trait trade-offs using Pearson correlation analysis and assessed their responses to environmental gradients using redundancy analysis (RDA). The results showed that trait variability differed markedly among marsh types. Inland salt marshes exhibited the greatest variation in leaf and root morphology, while morphological traits generally varied more strongly than ecophysiological traits. In inland salt marshes, root ecophysiological traits were closely associated with leaf morphology. Forested marshes showed multi-trait trade-offs and nutrient regulation, whereas freshwater herbaceous marshes displayed strong leaf-root trait correlations, indicating flexible inter-organ nutrient allocation. Soil environmental properties explained different proportions of functional trait variation across habitats, with the highest explanatory power in forested marshes (42.85%), followed by freshwater herbaceous marshes (34.56%) and inland salt marshes (34.41%). Soil carbon-to-nitrogen ratio significantly affected plant growth in all three habitats, although the other environmental drivers were habitat-specific. These findings reveal distinct community-level patterns of leaf–root trait variation and coordination among the sampled dominant herbaceous species across the three marsh habitats, providing empirical evidence for understanding wetland plant adaptation mechanisms in Northeast China. Full article

Dahlia is an economically important ornamental crop in the United States and worldwide. Dahlia is primarily propagated vegetatively through bulbs, which can result in vertical transmission of and constant accumulation of viruses. In this study, mosaic and yellowing symptoms were observed in Dahlia plants in a residential garden in California. Dahlia samples were collected and sequenced using high-throughput sequencing (HTS). HTS analysis was used to explore the Dahlia virome in the symptomatic plants, which revealed the presence of several contigs with homology to known Dahlia-infecting viruses, such as Dahlia common mosaic virus (DCMV) and tobacco streak virus (TSV), and Dahlia latent viroid. However, two contigs showed that they belong to the genera Potyvirus and Miraophiovirus with low to medium homology to previously known Potyvirus and Miraophiovirus species. Sequence identity showed that these two contigs represent two novel viruses tentatively named Dahlia potyvirus 1 (DaPoV1) and Dahlia miraophiovirus 1 (DaMV1). In addition, two other contigs showed low homology to a partitivirus and yue-like virus, indicating novelty. Mechanical inoculation of infected Dahlia tissue did not result in successful transmission onto indicator hosts. Electron microscopy of symptomatic plants showed typical potyviral-like flexuous virions. Phylogenetic analysis confirmed the placement of DaPoV1 and DaMV1 in their respective genera. This study represents a significant expansion of the known Dahlia virome and uncovers the complexity of viral infections where DCMV and TSV have been historically the primary focus in Dahlia. Widespread surveys should reveal the distribution of these viruses at commercial production sites. Full article

Urban blue–green spaces (UBGSs) are critical for biodiversity, yet our understanding of how plant diversity in these spaces responds to urbanization across different UBGS types remains limited. Here, we investigated plant communities in four sampled UBGSs along an urban gradient in the Minjiang River Basin, China. Because each blue–green space type is represented by a single site, all comparisons are site-specific and descriptive. Using species richness (S), the Shannon–Wiener index (H′), Pielou’s evenness index (J′), frequency (F), and importance value (I.V.), we described whether taxonomic composition and diversity patterns differed among the four sampled sites. Across all sites, based on 615 species records, angiosperms dominated (92.6%), and cosmopolitan families (e.g., Asteraceae, Poaceae, Fabaceae) were ubiquitous, consistent with a strong regional species pool influence. However, our cross-site comparison showed a contrasting pattern: the near-natural Baihetan National Wetland Park showed the highest S (12.87) but low evenness (0.49), whereas the intensively managed Huoshui Park, with the lowest richness (9.67), achieved the highest evenness (0.68). Further, a discordance between F and I.V. rankings was observed in the managed site, where ornamental species showed high spatial coverage but low I.V. ranks. We report this rank discordance only as an observational finding, not as a proven metric or indicator. This discordance suggests that richness alone may not fully capture community structure, though its diagnostic value remains unverified. We interpret these contrasting richness–evenness patterns from the four sampled sites. Because site identity is confounded with management intensity, habitat area, and landscape context, no causal attribution to ecological or anthropogenic factors can be made. Evenness and species richness showed different site-to-site patterns, but this association remains observational. We hypothesize that a resilient urban biodiversity network could combine complementary nodes, with large natural wetlands as species-rich sites and constructed parks as structurally balanced units, rather than pursuing a single diversity target. This study provides preliminary insights that may inform planning considerations under Chengdu’s Park City initiative, but these suggestions should be viewed as hypotheses requiring further validation rather than as established management guidelines. The proposed network approach, while conceptually appealing, requires empirical testing through replicated studies before it can be considered for broader application. Full article

The essential oil (EO) industry generates large volumes of solid distillation residues that are commonly discarded as waste, despite their potential as a source of nutrients and bioactive compounds. This study evaluated the chemical quality and the volatile profile of four types of exhausted biomasses (EBs) obtained after steam distillation of EOs from bay laurel (Laurus nobilis), lavender (Lavandula angustifolia), lavandin (L. angustifolia × L. latifolia) and two industrial hemp specimens (Carifit1p, a breeding line, and Codimono, a cultivar), with a view to their potential valorisation in animal feeding. The nutritional composition of the distillation residues was determined according to AOAC methods, while the headspace volatile fraction was characterised by HS-SPME–GC/MS. The EBs were generally characterised by a high fibre content, with the highest crude fibre and NDF values in whole lavender and lavandin plants, whereas the industrial hemp line Carifit1p showed the highest crude protein (17.1% DM) and ash contents. The volatile profiles of the residues differed markedly from those typical of the corresponding fresh EOs: the bay laurel residue was dominated by monoterpene hydrocarbons (74.5%) with strongly depleted 1,8-cineole (1.9%), while lavender and lavandin retained high levels of β-caryophyllene (35.8% and 32.4%, respectively). Industrial hemp residues were enriched in oxygenated sesquiterpenes, with caryophyllene oxide reaching 21.2% in Carifit1p and exceeding β-caryophyllene. Overall, the results indicate that EO exhausted biomasses retain a species-specific profile of fibre, residual nutrients and less volatile, less water-soluble terpenoids, supporting their potential reuse as feed ingredients and contributing to a more sustainable, near-zero-waste distillation supply chain. Full article

Soil salinization constrains global wheat production, but how organic–microbial amendments alleviate saline–alkali stress across plant tissues remains poorly characterized. In a single-season field trial, we compared three treatments—a non-saline control (CK), a saline–alkali control (SCK), and an organic fertilizer combined with a microbial inoculant applied to saline–alkali soil (SDM)—to evaluate effects on soil properties, the rhizosphere microbiome structure, leaf antioxidant defense, and grain quality. SDM improved soil nutrient availability: available phosphorus and potassium increased by 96.0% and 51.8%, respectively, relative to SCK, and rhizosphere microbiome shifts favored copiotrophic taxa (Flavobacterium, Devosia, Mortierella) over halotolerant genera (Arthrobacter, Filobasidium). In leaves, SDM induced a controlled antioxidant activation—peroxidase (POD) functioned as a key mediator of oxidative homeostasis, with activities exceeding CK yet remaining below extreme SCK levels—producing the lowest malondialdehyde content (−48.4% vs. SCK). At moderate concentrations, reactive oxygen species participated in developmental signaling rather than causing pathological damage, enabling grain soluble sugars and total starch to be restored to CK levels. These results reveal a coordinated soil–microbiome–plant response in which organic–microbial amendment enhances nutrient cycling, redirects community assembly toward nutrient-cycling functional guilds, and achieves oxidative homeostasis and grain quality restoration. Our findings offer a conceptual basis for the microbiome-informed management of saline–alkali wheat production. Full article

Distinguishing crop from weed species at the seedling stage is a fine-grained morphological discrimination problem. We study it on the public Plant Seedlings benchmark, which contains twelve species (three crops and nine weeds) imaged at the seedling stage. Automated classifiers report near-ceiling accuracy on this benchmark. Yet its images are acquired in controlled trays containing soil, gravel, rulers, barcodes, and printed labels, so a model may identify a species from its acquisition context rather than its morphology. We audit two architectures, EfficientNet-B7 and ViT-B/16, trained on the V2 dataset (5539 images) and probed with plant-only, background-only, and background-swapped inputs. Near-ceiling models (96.1% and 96.4% over three seeds) recover the correct species for up to 47.7% of samples from the background alone (chance 8.3%) and lose about 60 points under background swapping. Context reliance is therefore a property of the benchmark, not any single architecture. Tracing this to its source, an independent learned representation of the plant-free background alone identifies the species at 72.4%. The reliance is correctable end to end: a consistency-regularisation scheme retains 92.1% full-image accuracy for the transformer with no segmentation at inference, at an architecture-dependent cost. Reported accuracy thus partly measures acquisition context, not morphology; morphological grounding should be measured and reported alongside accuracy. Full article