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Sensors 2016, 16(9), 1355;

Bioethanol in Biofuels Checked by an Amperometric Organic Phase Enzyme Electrode (OPEE) Working in “Substrate Antagonism” Format

Department of Chemistry, University of Rome “Sapienza”, P.le A. Moro 5, Rome 00185, Italy
Author to whom correspondence should be addressed.
Academic Editor: Roberto Pilloton
Received: 15 July 2016 / Revised: 16 August 2016 / Accepted: 17 August 2016 / Published: 25 August 2016
(This article belongs to the Special Issue Amperometric Biosensors)
PDF [1171 KB, uploaded 25 August 2016]


The bioethanol content of two samples of biofuels was determined directly, after simple dilution in decane, by means of an amperometric catalase enzyme biosensor working in the organic phase, based on substrate antagonisms format. The results were good from the point of view of accuracy, and satisfactory for what concerns the recovery test by the standard addition method. Limit of detection (LOD) was on the order of 2.5 × 10−5 M. View Full-Text
Keywords: catalase OPEE; biofuels; bioethanol; determination catalase OPEE; biofuels; bioethanol; determination

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tomassetti, M.; Spuri Capesciotti, G.; Angeloni, R.; Martini, E.; Campanella, L. Bioethanol in Biofuels Checked by an Amperometric Organic Phase Enzyme Electrode (OPEE) Working in “Substrate Antagonism” Format. Sensors 2016, 16, 1355.

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