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Open AccessArticle

Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

1
Center for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, Russia
2
Department of Pathophysiology, Siberian State Medical University, 634050 Tomsk, Russia
3
Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, SB RAS, 634055 Tomsk, Russia
4
Research School of High-Energy Physics, Tomsk Polytechnic University, 634055 Tomsk, Russia
5
Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, Russia
6
Department of Morphology and General Pathology, Siberian State Medical University, 634050 Tomsk, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(20), 7682; https://doi.org/10.3390/ijms21207682
Received: 17 August 2020 / Revised: 2 October 2020 / Accepted: 14 October 2020 / Published: 16 October 2020
(This article belongs to the Special Issue Medical Genetics, Genomics and Bioinformatics – 2020)
The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (RUNX2, BMP2, BMP6, BGLAP, and ALPL) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches. View Full-Text
Keywords: human adipose tissue; stem cell culture; cytokine/chemokine genes; osteogenic genes; microarc oxidation technique; technological coating properties; correlations human adipose tissue; stem cell culture; cytokine/chemokine genes; osteogenic genes; microarc oxidation technique; technological coating properties; correlations
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Litvinova, L.; Yurova, K.; Shupletsova, V.; Khaziakhmatova, O.; Malashchenko, V.; Shunkin, E.; Melashchenko, E.; Todosenko, N.; Khlusova, M.; Sharkeev, Y.; Komarova, E.; Sedelnikova, M.; Khlusov, I. Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating. Int. J. Mol. Sci. 2020, 21, 7682.

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