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Open AccessArticle

Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis

1
Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele, Romania
2
Faculty of Applied Sciences, University Politehnica of Bucharest, Splaiul Independentei 313, RO-060042 Bucharest, Romania
3
Department of Life and Environmental Physics, Horia Hulubei National Institute for Physics and Nuclear Engineering, Reactorului 30, RO-077125 Magurele, Romania
4
Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, Gheorghe Polizu 1-7, RO-011061 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6426; https://doi.org/10.3390/ijms21176426
Received: 29 July 2020 / Revised: 28 August 2020 / Accepted: 2 September 2020 / Published: 3 September 2020
(This article belongs to the Special Issue Polymeric Scaffolds: Design, Processing, and Biomedical Application)
The fabrication of 3D microstructures is under continuous development for engineering bone substitutes. Collagen/chitosan (Col/CT) blends emerge as biomaterials that meet the mechanical and biological requirements associated with bone tissue. In this work, we optimize the osteogenic effect of 3D microstructures by their functionalization with Col/CT blends with different blending ratios. The structures were fabricated by laser direct writing via two-photons polymerization of IP-L780 photopolymer. They comprised of hexagonal and ellipsoidal units 80 µm in length, 40 µm in width and 14 µm height, separated by 20 µm pillars. Structures’ functionalization was achieved via dip coating in Col/CT blends with specific blending ratios. The osteogenic role of Col/CT functionalization of the 3D structures was confirmed by biological assays concerning the expression of alkaline phosphatase (ALP) and osteocalcin secretion as osteogenic markers and Alizarin Red (AR) as dye for mineral deposits in osteoblast-like cells seeded on the structures. The structures having ellipsoidal units showed the best results, but the trends were similar for both ellipsoidal and hexagonal units. The strongest osteogenic effect was obtained for Col/CT blending ratio of 20/80, as demonstrated by the highest ALP activity, osteocalcin secretion and AR staining intensity in the seeded cells compared to all the other samples. View Full-Text
Keywords: laser direct writing; 3D structure; collagen/chitosan functionalization; osteogenesis laser direct writing; 3D structure; collagen/chitosan functionalization; osteogenesis
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MDPI and ACS Style

Păun, I.A.; Mustăciosu, C.C.; Popescu, R.C.; Călin, B.Ş.; Mihăilescu, M. Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis. Int. J. Mol. Sci. 2020, 21, 6426. https://doi.org/10.3390/ijms21176426

AMA Style

Păun IA, Mustăciosu CC, Popescu RC, Călin BŞ, Mihăilescu M. Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis. International Journal of Molecular Sciences. 2020; 21(17):6426. https://doi.org/10.3390/ijms21176426

Chicago/Turabian Style

Păun, Irina A.; Mustăciosu, Cosmin C.; Popescu, Roxana C.; Călin, Bogdan Ş.; Mihăilescu, Mona. 2020. "Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis" Int. J. Mol. Sci. 21, no. 17: 6426. https://doi.org/10.3390/ijms21176426

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