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Open AccessArticle

BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

1
Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China
2
Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, China
3
Department of Horticulture, College of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6428; https://doi.org/10.3390/ijms21176428
Received: 18 July 2020 / Revised: 19 August 2020 / Accepted: 31 August 2020 / Published: 3 September 2020
(This article belongs to the Section Molecular Plant Sciences)
Chinese cabbage (Brassica campestris) is an economically important leaf vegetable crop worldwide. Mounting studies have shown that cysteine-cysteine-cysteine-histidine (CCCH) zinc-finger protein genes are involved in various plant growth and development processes. However, research on the involvement of these genes in male reproductive development is still in its infancy. Here, we identified 11 male fertility-related CCCH genes in Chinese cabbage. Among them, a pair of paralogs encoding novel non-tandem CCCH zinc-finger proteins, Brassica campestris Male Fertility 30a (BcMF30a) and BcMF30c, were further characterized. They were highly expressed in pollen during microgametogenesis and continued to express in germinated pollen. Further analyses demonstrated that both BcMF30a and BcMF30c may play a dual role as transcription factors and RNA-binding proteins in plant cells. Functional analysis showed that partial bcmf30a bcmf30c pollen grains were aborted due to the degradation of pollen inclusion at the microgametogenesis phase, and the germination rate of viable pollen was also greatly reduced, indicating that BcMF30a and BcMF30c are required for both pollen development and pollen germination. This research provided insights into the function of CCCH proteins in regulating male reproductive development and laid a theoretical basis for hybrid breeding of Chinese cabbage. View Full-Text
Keywords: CCCH zinc-finger protein; Brassica campestris ssp. chinensis; pollen development; pollen germination; male fertility CCCH zinc-finger protein; Brassica campestris ssp. chinensis; pollen development; pollen germination; male fertility
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MDPI and ACS Style

Xu, L.; Xiong, X.; Liu, W.; Liu, T.; Yu, Y.; Cao, J. BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis. Int. J. Mol. Sci. 2020, 21, 6428. https://doi.org/10.3390/ijms21176428

AMA Style

Xu L, Xiong X, Liu W, Liu T, Yu Y, Cao J. BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis. International Journal of Molecular Sciences. 2020; 21(17):6428. https://doi.org/10.3390/ijms21176428

Chicago/Turabian Style

Xu, Liai; Xiong, Xingpeng; Liu, Weimiao; Liu, Tingting; Yu, Youjian; Cao, Jiashu. 2020. "BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis" Int. J. Mol. Sci. 21, no. 17: 6428. https://doi.org/10.3390/ijms21176428

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