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Solid-Phase Chemical Synthesis of Stable Isotope-Labeled RNA to Aid Structure and Dynamics Studies by NMR Spectroscopy

Center for Biomolecular Structure and Organization, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Ramon Eritja
Molecules 2019, 24(19), 3476; https://doi.org/10.3390/molecules24193476
Received: 1 September 2019 / Revised: 22 September 2019 / Accepted: 23 September 2019 / Published: 25 September 2019
RNA structure and dynamic studies by NMR spectroscopy suffer from chemical shift overlap and line broadening, both of which become worse as RNA size increases. Incorporation of stable isotope labels into RNA has provided several solutions to these limitations. Nevertheless, the only method to circumvent the problem of spectral overlap completely is the solid-phase chemical synthesis of RNA with labeled RNA phosphoramidites. In this review, we summarize the practical aspects of this methodology for NMR spectroscopy studies of RNA. These types of investigations lie at the intersection of chemistry and biophysics and highlight the need for collaborative efforts to tackle the integrative structural biology problems that exist in the RNA world. Finally, examples of RNA structure and dynamic studies using labeled phosphoramidites are highlighted. View Full-Text
Keywords: stable isotope; phosphoramidite; solid-phase synthesis; NMR spectroscopy; RNA; structure; dynamics stable isotope; phosphoramidite; solid-phase synthesis; NMR spectroscopy; RNA; structure; dynamics
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Becette, O.; Olenginski, L.T.; Dayie, T.K. Solid-Phase Chemical Synthesis of Stable Isotope-Labeled RNA to Aid Structure and Dynamics Studies by NMR Spectroscopy. Molecules 2019, 24, 3476.

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