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13 Results Found

  • Article
  • Open Access
51 Citations
13,148 Views
11 Pages

High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity

  • Blanca Iglesias-Figueroa,
  • Norberto Valdiviezo-Godina,
  • Tania Siqueiros-Cendón,
  • Sugey Sinagawa-García,
  • Sigifredo Arévalo-Gallegos and
  • Quintín Rascón-Cruz

In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant...

  • Article
  • Open Access
8 Citations
4,884 Views
23 Pages

Digestive Profiles of Human Milk, Recombinant Human and Bovine Lactoferrin: Comparing the Retained Intact Protein and Peptide Release

  • Bum Jin Kim,
  • Russell F. Kuhfeld,
  • Joanna L. Haas,
  • Yanisa M. Anaya,
  • Raysa Rosario Martinez,
  • Baidya Nath P. Sah,
  • Bella Breen,
  • Kahler Newsham,
  • Carrie-Anne Malinczak and
  • David C. Dallas

21 July 2024

Lactoferrin (LF) is a major component of human milk. LF supplementation (currently bovine) supports the immune system and helps maintain iron homeostasis in adults. No recombinant human lactoferrin (rhLF) is available for commercial food use. To dete...

  • Review
  • Open Access
16 Citations
6,325 Views
26 Pages

In this article, we review the benefits of application of colostrum and colostrum-derived proteins in animal models and clinical trials that include chemotherapy with antimetabolic drugs, radiotherapy and surgical interventions. A majority of the rep...

  • Article
  • Open Access
11 Citations
2,889 Views
9 Pages

Human Recombinant Lactoferrin Promotes Differentiation and Calcification on MC3T3-E1 Cells

  • Daichi Nagashima,
  • Yukiko Ishibashi,
  • Sachiko Kawaguchi,
  • Megumi Furukawa,
  • Masahiro Toho,
  • Megumi Ohno,
  • Takeaki Nitto and
  • Nobuo Izumo

Lactoferrin (LF), known to be present in mammalian milk, has been reported to promote the proliferation of osteoblasts and suppress bone resorption by affecting osteoclasts. However, the mechanisms underlying the effects of human sources LF on osteob...

  • Article
  • Open Access
1 Citations
2,599 Views
15 Pages

Immunologic Effects of a Novel Bovine Lactoferrin-Derived Peptide on the Gut and Clinical Perspectives

  • Haiyue Cui,
  • Huan Yang,
  • Xiaoxi Qi,
  • Yang Zhao,
  • Tianle Huang and
  • Liguang Miao

6 November 2024

Bovine lactoferrin is a natural iron-binding glycoprotein known for its antimicrobial, antiviral, antitumor, anti-inflammatory, and immunomodulatory properties. In this study, we artificially recombined a fragment of bovine lactoferrin with immunomod...

  • Review
  • Open Access
13 Citations
11,330 Views
26 Pages

In this article, we review the benefits of applying bovine colostrum (BC) and lactoferrin (LF) in animal models and clinical trials that include corticosteroid application and psychic stress, treatment with non-steroid anti-inflammatory drugs (NSAIDs...

  • Article
  • Open Access
3 Citations
2,196 Views
12 Pages

The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off

  • Sílvia Parés,
  • Olivia Cano-Garrido,
  • Alex Bach,
  • Neus Ferrer-Miralles,
  • Antonio Villaverde,
  • Elena Garcia-Fruitós and
  • Anna Arís

30 November 2021

The dry period is decisive for the milking performance of dairy cows. The promptness of mammary gland involution at dry-off affects not only the productivity in the next lactation, but also the risk of new intra-mammary infections since it is closely...

  • Article
  • Open Access
12 Citations
3,126 Views
15 Pages

We have recently developed probiotics that can express bovine, human, or porcine lactoferrin (LF), and the present study evaluated the effect of these probiotics in improving non-alcoholic fatty liver disease (NAFLD). Three kinds of probiotic supplem...

  • Article
  • Open Access
9 Citations
3,718 Views
19 Pages

A Single Plasmid of Nisin-Controlled Bovine and Human Lactoferrin Expressing Elevated Antibacterial Activity of Lactoferrin-Resistant Probiotic Strains

  • Zhen-Shu Liu,
  • Chuen-Fu Lin,
  • Chung-Pei Lee,
  • Min-Chi Hsieh,
  • Hung-Fu Lu,
  • Ying-Fang Chen,
  • Yu-We Ku and
  • Po-Wen Chen

Lactoferrin (LF) is a multifunctional protein found in mammals, and it shows broad-spectrum antimicrobial activity. To improve the functional properties of specific probiotics in order to provide both the beneficial characteristics of lactic acid bac...

  • Article
  • Open Access
1,240 Views
14 Pages

Inhibitory Effects of Bovine Lactoferricin-Lactoferrampin on Senecavirus A and Foot-and-Mouth Disease Virus with Recombinant Lactobacillus Oral Treatment in Mice

  • Wenyue Zhao,
  • Senhao Zhang,
  • Ling Sui,
  • Xiaona Wang,
  • Jiaxuan Li,
  • Wen Cui,
  • Yanping Jiang,
  • Xinyuan Qiao and
  • Lijie Tang

25 February 2025

Foot-and-mouth disease virus (FMDV) and Senecavirus A (SVA) have similar pathogenic characteristics, and both are important pathogens that harm the livestock industry. Studies have shown that lactoferrin peptides can inhibit the replication of variou...

  • Article
  • Open Access
3 Citations
2,439 Views
20 Pages

Optimum Fermentation Conditions for Bovine Lactoferricin-Lactoferrampin-Encoding LimosiLactobacillus reuteri and Regulation of Intestinal Inflammation

  • Weichun Xie,
  • Xueying Wang,
  • Jiyao Cai,
  • Huitao Bai,
  • Yilan Shao,
  • Zhuoran Li,
  • Limeng Cai,
  • Senhao Zhang,
  • Jiaxuan Li and
  • Lijie Tang
  • + 1 author

9 November 2023

The multifunctional antibacterial peptide lactoferricin-lactoferrampin (LFCA) is derived from bovine lactoferrin. Optimization of the fermentation process should be studied since different microorganisms have their own favorable conditions and proces...

  • Article
  • Open Access
12 Citations
5,458 Views
15 Pages

2 February 2024

Lactoferrin (LF) stands as one of the extensively investigated iron-binding glycoproteins within milk, exhibiting diverse biological functionalities. The global demand for LF has experienced consistent growth. Biotechnological strategies aimed at enh...

  • Article
  • Open Access
12 Citations
3,570 Views
17 Pages

Milk Allergen Micro-Array (MAMA) for Refined Detection of Cow’s-Milk-Specific IgE Sensitization

  • Victoria Garib,
  • Daria Trifonova,
  • Raphaela Freidl,
  • Birgit Linhart,
  • Thomas Schlederer,
  • Nikolaos Douladiris,
  • Alexander Pampura,
  • Daria Dolotova,
  • Tatiana Lepeshkova and
  • Rudolf Valenta
  • + 7 authors

21 May 2023

Background: Immunoglobulin-E(IgE)-mediated hypersensitivity to cow’s milk allergens is a frequent cause of severe and life-threatening anaphylactic reactions. Besides case histories and controlled food challenges, the detection of the IgE antib...