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10 Results Found

  • Article
  • Open Access
1 Citations
1,067 Views
19 Pages

17 July 2025

This study discloses the noncovalent immobilization of a bienzyme cascade composed of glucose oxidase (GOx) and horseradish peroxidase (HRP) onto magnetically responsive polyamide microparticles (PA MPs). Porous PA6, PA4, and PA12 MPs containing iron...

  • Article
  • Open Access
5 Citations
2,343 Views
13 Pages

20 March 2024

We describe the creation of a conductive microcavity based on the assembly of two pieces of carbon nanotube buckypaper for the entrapment of two enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx), as well as a redox mediator: 2,2′-...

  • Article
  • Open Access
31 Citations
5,753 Views
22 Pages

Development of a Four-Enzyme Magnetic Nanobiocatalyst for Multi-Step Cascade Reactions

  • Archontoula Giannakopoulou,
  • Michaela Patila,
  • Konstantinos Spyrou,
  • Nikolaos Chalmpes,
  • Dimitra Zarafeta,
  • Georgios Skretas,
  • Dimitrios Gournis and
  • Haralambos Stamatis

27 November 2019

We report the preparation, characterization and application of a novel magnetic four-enzyme nanobiocatalyst prepared by the simultaneous covalent co-immobilization of cellulase (CelDZ1), β-glucosidase (bgl), glucose oxidase (GOx) and horseradish...

  • Article
  • Open Access
3 Citations
3,241 Views
32 Pages

Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System

  • Tim Wendlandt,
  • Claudia Koch,
  • Beate Britz,
  • Anke Liedek,
  • Nora Schmidt,
  • Stefan Werner,
  • Yuri Gleba,
  • Farnoosh Vahidpour,
  • Melanie Welden and
  • Christina Wege
  • + 4 authors

19 September 2023

Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The la...

  • Article
  • Open Access
795 Views
17 Pages

A Biofuel Cell for Electricity Generation from Biomass-Derived Cellobiose

  • Piyanut Pinyou,
  • Peeranat Jatooratthawichot,
  • Luciranon Sribrahma,
  • Salila Pengthaisong,
  • Chamaipon Beagbandee,
  • Kantapat Chansaenpak,
  • Vincent Blay and
  • James R. Ketudat Cairns

7 October 2025

We have developed a new bioanode based on a cascade of reactions catalyzed by two enzymes. A glassy carbon electrode is modified with β-glucosidase and glucose oxidase enzymes entrapped within an osmium redox polymer. Cellobiose, the fuel for th...

  • Communication
  • Open Access
2 Citations
3,364 Views
10 Pages

The Mn2BPMP complex has an intrinsic peroxidase-like activity in the pH range of 5 to 8, especially a maximum activity at pH 7, while most peroxidase mimics operate at an acidic pH (mainly pH 4). Its peroxidase-like activity is high among small-molec...

  • Article
  • Open Access
59 Citations
12,265 Views
12 Pages

Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell

  • Kantapat Chansaenpak,
  • Anyanee Kamkaew,
  • Sireerat Lisnund,
  • Pannaporn Prachai,
  • Patipat Ratwirunkit,
  • Thitichaya Jingpho,
  • Vincent Blay and
  • Piyanut Pinyou

7 January 2021

Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenas...

  • Article
  • Open Access
19 Citations
5,138 Views
16 Pages

Hydrogel Microvalves as Control Elements for Parallelized Enzymatic Cascade Reactions in Microfluidics

  • Franziska Obst,
  • Anthony Beck,
  • Chayan Bishayee,
  • Philipp J. Mehner,
  • Andreas Richter,
  • Brigitte Voit and
  • Dietmar Appelhans

5 February 2020

Compartmentalized microfluidic devices with immobilized catalysts are a valuable tool for overcoming the incompatibility challenge in (bio) catalytic cascade reactions and high-throughput screening of multiple reaction parameters. To achieve flow con...

  • Article
  • Open Access
8 Citations
3,002 Views
26 Pages

14 January 2023

This study reports on the synthesis of novel bienzyme polymer-assisted nanoflower complexes (PANF), their morphological and structural characterization, and their effectiveness as cascade biocatalysts. First, highly porous polyamide 6 microparticles...

  • Article
  • Open Access
9 Citations
3,416 Views
19 Pages

Development of a Multi-Enzymatic Biocatalytic System through Immobilization on High Quality Few-Layer bio-Graphene

  • Christina Alatzoglou,
  • Michaela Patila,
  • Archontoula Giannakopoulou,
  • Konstantinos Spyrou,
  • Feng Yan,
  • Wenjian Li,
  • Nikolaos Chalmpes,
  • Angeliki C. Polydera,
  • Petra Rudolf and
  • Haralambos Stamatis
  • + 1 author

26 December 2022

In this work, we report the green production of few-layer bio-Graphene (bG) through liquid exfoliation of graphite in the presence of bovine serum albumin. Microscopic characterization evaluated the quality of the produced nanomaterial, showing the p...