Search Results (258)

Ricinus communis (Euphorbiaceae), commonly known as the castor oil plant, is prized for its versatile applications in medicine, industry, and agriculture. It features large, deeply lobed leaves with vibrant colours, robust stems with anthocyanin pigments, and extensive root systems for nutrient absorption. Its terminal panicle-like inflorescences bear monoecious flowers, and its seeds are enclosed in prickly capsules. Throughout its various parts, R. communis harbours a diverse array of bioactive compounds. Leaves contain tannins, which exhibit astringent and antimicrobial properties, and alkaloids like ricinine, known for anti-inflammatory properties, as well as flavonoids like rutin, offering antioxidant and antibacterial properties. Roots contain ellagitannins, lupeol, and indole-3-acetic acid, known for anti-inflammatory and liver-protective effects. Seeds are renowned for ricin, ricinine, and phenolic compounds crucial for industrial applications such as biodegradable polymers. Pharmacologically, it demonstrates antioxidant effects from flavonoids and tannins, confirmed through minimum inhibitory concentration (MIC) assays for antibacterial activity. It shows potential in managing diabetes via insulin signalling pathways and exhibits anti-inflammatory properties by activating nuclear factor erythroid 2-related factor 2 (Nrf2). Additionally, it has anti-fertility effects and potential anticancer activity against cancer stem cells. This review aims to summarize Ricinus communis’s botanical properties, therapeutic uses, chemical composition, pharmacological effects, and industrial applications. Integrating the current knowledge offers insights into future research directions, emphasizing the plant’s diverse roles in agriculture, medicine, and industry. Full article

In the context of modern dermocosmetic development, multifunctional topical gel-cream formulations must be efficient for both therapeutic efficacy and cosmetic applications. This study presents a comparative physicochemical and pharmacotechnical analysis of three topical gel-cream formulations developed by Brand Chanand^®: Acne Control Cleanser (ACC), Acne Face Cream (AFC), and Gentle Cream Cleanser Serum Control, Regenerating, Hydrating, Calming (IRC). Each formulation is enriched with a specific blend of bioactive compounds, including botanical oils, vitamins, and proteins, designed to treat acne, to support skin regeneration, and to maintain the skin barrier. A multidisciplinary approach was used, including Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR), differential scanning calorimetry (DSC), rheological evaluation, pH and density determination, spreadability analysis, and oxidative stability testing to evaluate the products. Antioxidant capacity was assessed through multiple in vitro assays. The results demonstrated that all three gel-cream formulations exhibit pseudoplastic rheological behaviour, suitable for topical application. AFC showed the highest oxidative stability and antioxidant activity, while IRC presented superior spreadability and cosmetic efficacy, likely due to its complex composition. ACC displayed faster absorption and was ideal for targeted use on oily or acne-prone skin. The differences observed in the stability and performance suggest that the ingredient synergy, base composition, and solubility profiles show notable variations in dermato-cosmetic formulations. These findings highlight the formulation–performance relationship in topical gel-cream formulations and support the development of new cosmetic products tailored for sensitive and acne-prone skin. Full article

Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI) are powerful techniques that have been employed to analyze foodstuffs comprehensively. These techniques offer in-depth information about the chemical composition, structure, and spatial distribution of components in a variety of food products. Quantitative NMR is widely applied for precise quantification of metabolites, authentication of food products, and monitoring of food quality. Low-field ¹H-NMR relaxometry is an important technique for investigating the most abundant components of intact foodstuffs based on relaxation times and amplitude of the NMR signals. In particular, information on water compartments, diffusion, and movement can be obtained by detecting proton signals because of H₂O in foodstuffs. Saffron adulterations with calendula, safflower, turmeric, sandalwood, and tartrazine have been analyzed using benchtop NMR, an alternative to the high-field NMR approach. The fraudulent addition of Robusta to Arabica coffee was investigated by ¹H-NMR Spectroscopy and the marker of Robusta coffee can be detected in the ¹H-NMR spectrum. MRI images can be a reliable tool for appreciating morphological differences in vegetables and fruits. In kiwifruit, the effects of water loss and the states of water were investigated using MRI. It provides informative images regarding the spin density distribution of water molecules and the relationship between water and cellular tissues. ¹H-NMR spectra of aqueous extract of kiwifruits affected by elephantiasis show a higher number of small oligosaccharides than healthy fruits do. One of the frauds that has been detected in the olive oil sector reflects the addition of hazelnut oils to olive oils. However, using the NMR methodology, it is possible to distinguish the two types of oils, since, in hazelnut oils, linolenic fatty chains and squalene are absent, which is also indicated by the ¹H-NMR spectrum. NMR has been applied to detect milk adulterations, such as bovine milk being spiked with known levels of whey, urea, synthetic urine, and synthetic milk. In particular, T2 relaxation time has been found to be significantly affected by adulteration as it increases with adulterant percentage. The ¹H spectrum of honey samples from two botanical species shows the presence of signals due to the specific markers of two botanical species. NMR generates large datasets due to the complexity of food matrices and, to deal with this, chemometrics (multivariate analysis) can be applied to monitor the changes in the constituents of foodstuffs, assess the self-life, and determine the effects of storage conditions. Multivariate analysis could help in managing and interpreting complex NMR data by reducing dimensionality and identifying patterns. NMR spectroscopy followed by multivariate analysis can be channelized for evaluating the nutritional profile of food products by quantifying vitamins, sugars, fatty acids, amino acids, and other nutrients. In this review, we summarize the importance of NMR spectroscopy in chemical profiling and quality assessment of food products employing magnetic resonance technologies and multivariate statistical analysis. Full article

Objective: Eating habits established during adolescence play a crucial role in shaping both current and future health status. However, adolescents today appear to be increasingly adopting poorer dietary and lifestyle habits. This study aims to investigate eating behaviors and supplement use among adolescent students who participated in the EduALI nutrition education project. Methods: The project included first-year students from six sports-oriented scientific high schools in Florence. The students participated in six nutrition sessions, completing the International Physical Activity Questionnaire to assess physical activity performance, a questionnaire on dietary supplement use, and, to assess eating habits a Food Frequency Questionnaire (FFQ), as well as the Medi-Lite questionnaire to specifically assess adherence to the Mediterranean diet (MD). The data from the FFQ were compared with national dietary guidelines. Results: The study sample consisted of 83 students (69.9% male; average age: 13.8 ± 0.4 y). The results showed that 61.4% of participants had used supplements in the past year, most commonly mineral salts (56.6%) and vitamins (54.2%), followed by botanical products (22%), sports drinks (16%), proteins/amino acids (8%), and creatine (2%). Alarmingly, 27.7% used supplements without medical supervision. Students involved in individual sports had higher supplement consumption than those in team sports, especially creatine use. Eating habits revealed poor adherence to both the MD and Italian dietary guidelines, with deficiencies in fruits, vegetables, olive oil, fish, and legumes, and excessive intake of cheese, meat, especially red and processed meats. Conclusions: A high prevalence of supplement use among adolescents was observed, along with poor adherence to dietary guidelines and MD. These findings underscore the need for targeted, school- and sport-based interventions to enhance adolescents’ nutritional awareness and responsible supplement use. Full article

Lavandula angustifolia Mill., lavender, is an aromatic plant in the Lamiaceae family. Lavender, which is native to the Mediterranean region but cultivated throughout the world, is an important economic plant. Several studies have investigated two aspects of this aromatic plant: (1) which pollinators, particularly bees, pollinate lavender, and (2) the composition of lavender essential oil. However, little research has been conducted to investigate how pollination affects either the yield or phytochemistry of lavender. The current study, which was conducted in North America, investigates which bee species visit lavender and how pollination affects plant chemistry, specifically the essential oil produced by lavender. Over the course of the 5-week observational period, a total of 12 species (across 10 genera) of bees were identified visiting lavender. Compared to previous studies on cultivated lavender at the same site (Mt. Nebo Botanical Farm, Mona, UT), four bee species not previously observed on lavender were identified. These included Hoplitis producta, Nomada sp., Osmia trevoris, and Megachile snowi. Pollinated lavender, compared to lavender excluded from pollinators, produced more essential oil (yield (w/w) = 1.49% vs. 1.07%), lower relative amounts of linalool (35.4% vs. 39.9%), and higher relative amounts of linalyl acetate (21.3% vs. 16.8%). The findings of this study demonstrate the ecological interactions between pollinators and lavender, and how those interactions impact phytochemistry. Full article

Consumers are increasingly willing to pay a premium for high-quality extra virgin olive oils (HQ-EVOOs) with specific sensory or nutraceutical properties, and originating from particular botanical or geographical origins. Regarding geographic origin, Italy is one of the main producers, with many local production areas, each characterized by its own distinctive typicity. The aim of this study is the chemical, sensory, and nutraceutical profiling of HQ-EVOO produced over two production years in Montespertoli (province of Florence) by 12 producers involved in the “MontEspertOlio” project, funded by the Tuscan Region. Oils were produced based on a production process previously defined and specifically applied to this territory. The shelf-life of the oil was also evaluated over a 12-month period. Legal quality parameters were analyzed according to EU regulation. Phenolic compounds, tocopherols, fatty acid composition, and volatile compounds were analyzed using HPLC-DAD, HPLC-FLD, HS-SPME-GC-MS, and GC-FID, respectively. Finally, sensory analysis was conducted using the Panel Test method. Results showed that Montespertoli HQ-EVOO is characterized by distinctive sensory and chemical traits that fully match consumer preferences, even across two production years characterized by different growing conditions. The shelf-life performance was excellent over 12 months, also showing a protective effect of greater bottle sizes against oxidation. Full article

Νatural compounds such as lactic, acetic, formic, and oxalic acid and thymol are currently registered for use against Varroa destructor in apiaries in Europe. Complex botanical extracts are yet to be authorized, despite their beneficial ecofriendly profile and advantages in terms of resistance management. This study examined the fumigant activity of the essential oil (EO) of oregano, clove, lavender, dittany, bay laurel, sweet orange, peppermint, blue gum, and lemon balm against V. destructor in laboratory bioassays (Petri dishes). The most effective EOs were those of Origanum vulgare, Syzygium aromaticum, and Origanum dictamnus. These three EOs yielded 33.75% carvacrol, 58.64% eugenol, and 69.77% carvacrol and exhibited significant activity from 18 h of exposure to 0.0013 μL/cm until 48 h of exposure to 0.0068 μL/cm³. Origanum vulgare’s first calculated LC₅₀ value was 0.003 μL/cm³ after 24 h of mites’ exposure to EO vapors. The LC₅₀ values stabilized for oregano, clove, and dittany at 0.001, 0.002, and 0.002 μL/cm³ of 24 h exposure, respectively. This first indication of fumigant miticidal activity in Petri dishes is a promising first step before scaling up to field experiments. Full article

Starch, as a natural, low-cost, and vegan-friendly raw material, aligns well with the growing demand for sustainable, zero-waste, and waterless cosmetic products. Its biodegradability and natural origin allow for minimal environmental impact during production and disposal. Anhydrous lotion body bars, solid and water-free alternatives to traditional moisturizers, offer high concentrations of active ingredients that are more effective and have a longer shelf life. Their solid form enables packaging in paper-based containers, reducing plastic waste. To address formulation challenges such as excessive greasiness, poor absorption, or lack of structural stability, which are often associated with the high oil content of anhydrous body lotion bars, starch may serve as a promising natural additive. The aim of this study was to optimize the formulation of an innovative starch-based anhydrous lotion bar. For this purpose, physicochemical analyses of starches from various botanical sources (corn, rice, tapioca, waxy corn and potato) were performed, along with evaluations of the functional (including commercially acceptable form, hardness sufficient for application, product stability, reduced greasiness and stickiness) and mechanical properties of the resulting bars. Additionally, the rheological behavior was described using the De Kee model. The results indicate that a 2.5% starch addition, regardless of its botanical origin, provides the best balance between viscosity and ease of application. Moreover, starches with a low moisture content and high oil absorption capacity effectively reduce the greasy skin sensation. These findings demonstrate the potential of starch as a natural multifunctional additive in the development of stable, user-friendly anhydrous lotion body bars. Full article

Chrysanthemum species represent an economically important group of flowering plants. Many species also present a medicinal interest, notably for the treatment of inflammatory pathologies. This is the case for Chrysanthemum boreale Makino, endemic to Japan and widespread in Eastern Asia. This perennial plant has long been used in folk medicine to treat inflammatory diseases and bacterial infections. An extensive review of the scientific literature pertaining to C. boreale has been performed to analyze the origin of the plant, its genetic traits, the traditional usages, and the properties of aqueous or organic plant extracts and essential oils derived from this species. Aqueous extracts and the associated flavonoids, such as acacetin and glycoside derivatives, display potent antioxidant activities. These aqueous extracts and floral waters are used mainly as cytoprotective agents. Organic extracts, in particular those made from methanol or ethanol, essentially display antioxidant and anti-inflammatory properties useful to protect organs from oxidative damage. They can be used for neuroprotection. Essential oils from C. boreale have been used as cytoprotective or antibacterial agents. The main bioactive natural products isolated from the plant include flavonoids such as acacetin and related glycosides (notably linarin), and diverse sesquiterpene lactones (SLs). Among monomeric SLs, cumambrins and borenolide are the main products of interest, with cumambrin A targeting covalently the transcription factor NF-κB to regulate proinflammatory gene expression to limit osteoclastic bone resorption. The dimeric SL handelin, which is characteristic of C. boreale, exhibits a prominent anti-inflammatory action, with a capacity to target key proteins like kinase TAK1 and chaperone Hsp70. A few other natural products isolated from the plant (tulipinolide, polyacetylenic derivatives) are discussed. Altogether, the review explores all medicinal usages of the plant and the associated phytochemical panorama, with the objective of promoting further botanical and chemical studies of this ancestral medicinal species. Full article

Background: Essential oils are widely studied for their therapeutic potential, including their role in wound healing. Myristica fragrans essential oil (MEO) has been previously investigated for various pharmacological activities, including anti-inflammatory and antimicrobial effects. However, its mechanistic role in accelerating wound healing and modulating critical pathways, such as oxidative stress, inflammation, and apoptosis, remains poorly characterized. MEO contains a rich profile of monoterpene esters, sesquiterpenoids, and phenolic acids, which may contribute to its bioactivity through unique multi-targeted mechanisms. Objective: This research aims to investigate the curative properties of MEO on wound repair, specifically its capacity to regulate inflammation, oxidative stress, and apoptosis in an excision wound model using Wistar rats. Methods: Chemical characterization via GC-MS analysis identified Nitrobenzoate Esters (12.85%), Terpenoid/Cineole (6.99%), and Gamma-Terpinene (4.67%) as the dominant constituents. This study utilized a full-thickness excision wound model, and wound contraction, inflammatory cytokines (IL-1β and TNF-α), a macrophage cell surface marker (CD68), oxidative stress markers (ROS MDA, SOD, GSH), and apoptotic regulation (Caspase-3) was evaluated using macroscopic, histopathological, and immunohistochemical analyses. Result: MEO treatment significantly reduced pro-inflammatory cytokines IL-1β (658.3 ± 32.7 pg/mg, *** p < 0.005) and TNF-α (266.7 ± 33.3 pg/mg, *** p < 0.005), compared to the control group (983.3 ± 60.1 and 650 ± 42.8 ** p < 0.05, respectively). CD68 expression was also markedly decreased (12.67 ± 0.71 ng/mL, *** p < 0.005). Furthermore, MEO effectively attenuated oxidative stress by reducing ROS and MDA levels while restoring antioxidant enzymes GSH and SOD. Conclusions: This study demonstrates that Mace Essential Oil (MEO) effectively promotes wound healing by modulating inflammation, oxidative stress, and apoptosis in a preclinical rat model. Its unique bioactive components suggest significant therapeutic potential as a botanical agent for skin repair. Further research is warranted to explore its application in advanced wound-care formulations. Full article

The tomato leaf miner Phthorimaea (syn. Tuta) absoluta (Lepidoptera: Gelechiidae) is invasive in many agricultural regions. Its larvae feed inside leaf mines and tomato fruits, causing yield losses. Repeated sprays of insecticides disrupt agri-ecosystems. Conducting three open-field tomato experiments, we assessed whether formulations of entomopathogenic nematodes could improve the efficacy of these promising biocontrol agents or whether other nature-based agents such as pyrethrin or spinosad would be the better option(s), as compared to a standard insecticide. Steinernema carpocapsae formulated in an alkyl polyglycoside polymeric surfactant, in canola oil, or mixed in both reduced 37 to 68% of pest larvae within two weeks post-treatment, followed by the botanical pyrethrin (48%). Neither spinosad nor lambda cyhalothrin achieved sufficient control. Increasing the frequency of treatments to every two weeks moderately increased efficacy. Positively, the nematodes can, if properly formulated and applied, still be recovered alive from leaf surfaces up to two hours after spraying, indicating that they have time to enter the leaf mines. A small proportion can even be still extracted alive from the leaf mines one week later. Despite these promising results, further research is needed to improve the efficacy of nature-based management options for use against this pest, with the aim of ultimately reducing reliance on chemical insecticides and minimizing the impact on agri-ecosystems. Full article

This study evaluated the repellent activity of DEET, eugenol, methyl eugenol, 1,8-cineole, and their combinations against Amblyomma sculptum nymphs through laboratory and field tests. Initially, the botanical compounds were tested individually in Petri dish assays to assess repellent activity over various intervals (1 min to 168 h) at concentrations of 2%. Eugenol and methyl eugenol exhibited the highest average repellent percentages (60 to 90%), with significant effects (p < 0.05) across most evaluation periods, in contrast to 1,8-cineole. Therefore, eugenol and methyl eugenol were selected for combination testing with DEET. The combinations of 7% DEET + 1% eugenol and 7% DEET + 2% methyl eugenol showed the best results, with average repellent efficacy exceeding 85%. The first combination was selected for field evaluation. In this test, volunteers wore treated socks and walked for two hours in a naturally infested area. A significant reduction (p < 0.05) in the number of ticks recovered from the socks was observed in the 7% DEET and 7% DEET + 1% eugenol treatments, with efficacies of 82.5% and 88.5%, respectively, after 120 min. Isolated eugenol showed limited field efficacy, with significant repellent activity (p < 0.05) lasting only 20 min. The results highlight the potential of eugenol and methyl eugenol as repellent compounds, especially when combined with DEET. The field study confirmed the efficacy of the 7% DEET + 1% eugenol. Full article

The tomato russet mite (TRM), Aculops lycopersici, is a destructive pest of tomato crops worldwide. It poses a significant challenge to growers in both greenhouse and open-field conditions. Traditional chemical control methods are often ineffective, promote resistance, and have negative environmental impacts. This has prompted the search for alternative strategies, such as biological control and eco-friendly botanical pesticides. In this study, we evaluated the acaricidal effects of essential oils (EOs) extracted from three officinal plants, Origanum vulgare L., Salvia rosmarinus Spenn., and Salvia officinalis L., cultivated using precision aromatic crop (PAC) techniques. Their efficacy was evaluated against A. lycopersici under laboratory conditions. The chemical composition of the EOs was determined by solid-phase microextraction (SPME) coupled with gas chromatography–mass spectrometry (GC-MS). The dominant component of O. vulgare EO was carvacrol (83.42%), followed by ρ-cymene (3.06%), and γ-terpinene (2.93%). In S. rosmarinus, α-pinene (28.0%), 1,8-cineole (11.00%), and borneol (7.72%) were the major components. S. officinalis EO was characterized by high levels of 1,8-cineole (27.67%), camphor (21.91%), and crisantenone (12.87%). We tested multiple concentrations (320–5000 μL L−¹) and exposure times (1–4 days) to assess mite mortality. The results revealed both dose- and time-dependent toxic activity, with significant differences among EOs. O. vulgare EO was the most toxic, causing 90% mortality at 0.5% (w/v) concentration after 4 days. S. rosmatinus and S. officinalis EOs had more limited effects, with 46% and 42% mortality, respectively. Lethal concentration (LC₅₀) values were 2.23 mL L−¹ (95% CI: 1.74–3.05) for O. vulgare, 5.84 mL L−¹ (95% CI: 3.28–22.29) for S. rosmarinus, and 6.01 mL L−¹ (95% CI: 2.63–261.60) for S. officinalis. These results indicate that O. vulgare EO shows efficacy comparable to commercially available botanical pesticides. Our findings support the potential of O. vulgare EO as a viable alternative for the control of A. lycopersici, contributing to integrated pest management (IPM) strategies. Full article

Biofilms, structured microbial consortia embedded in self-produced extracellular matrices, pose significant challenges across the medical, industrial, and environmental sectors due to their resistance to antimicrobial therapies and ability to evade the immune system. Their resilience is driven by multifaceted mechanisms, including matrix-mediated drug sequestration, metabolic dormancy, and quorum sensing (QS)-regulated virulence, which collectively sustain persistent infections and contribute to the amplification of antimicrobial resistance (AMR). This review critically examines the potential of plant-derived essential oils (EOs) as innovative agents for biofilm control. EOs exhibit broad-spectrum antibiofilm activity through multi-target mechanisms, including disrupting initial microbial adhesion, degrading extracellular polymeric substances (EPSs), suppressing QS pathways, and compromising membrane integrity. Their ability to act synergistically with conventional antimicrobials at sub-inhibitory concentrations enhances therapeutic efficacy while reducing the selection pressure for resistance. Despite their potential, EO applications face technical challenges, such as compositional variability due to botanical sources, formulation stability issues, and difficulties in standardization for large-scale production. Clinical translation is further complicated by biofilm stage- and strain-dependent efficacy, insufficient in vivo validation of therapeutic outcomes, and potential cytotoxicity at higher doses. These limitations underscore the need for optimized delivery systems, such as nanoencapsulation, to enhance bioavailability and mitigate adverse effects. Future strategies should include combinatorial approaches with antibiotics or EPS-degrading enzymes, advanced formulation technologies, and standardized protocols to bridge laboratory findings to clinical practice. By addressing these challenges, EOs hold transformative potential to mitigate biofilm-associated AMR, offering sustainable, multi-target alternatives for infection management and biofilm prevention in diverse contexts. Full article

Background: Zinc oxide paste is traditionally compounded and applied in the therapy of various skin conditions. However, prolonged use of talc, usually present in zinc oxide pastes, may pose health risks due to potential contamination with asbestos and quartz, highlighting the need for alternative excipients. This study aimed to examine the effects of starches from various botanical sources and their particle size on the rheological and absorption properties of zinc oxide paste. Methods: Eight zinc oxide paste formulations were prepared, containing 25% zinc oxide and 25% indifferent excipient (talc, tapioca, rice, or maize starch) in two particle sizes. Rheological properties were assessed using amplitude and frequency sweep tests, and water- and oil-absorption capacities were determined using a centrifugation-based method. Results: Amplitude sweep tests confirmed the predominant solid-like nature of zinc oxide pastes, with the elastic modulus (G′) exceeding the viscous modulus (G″) in all formulations. Tapioca starch-based pastes exhibited the highest G′ and G″ values, while talc-based pastes exhibited the lowest. Frequency sweep tests showed that pastes were resistant to structural changes under stress, with G′ consistently dominating over G″ across the entire frequency range. Tapioca starch-based formulations exhibited the highest water-absorption capacity, while the talc-based formulations had the highest oil-absorption capacity. Reducing particle size improved both water- and oil-absorption capacities. Conclusions: Starches may be considered as alternatives to talc in zinc oxide pastes, due to their ability to modify the absorption and rheological properties of pastes. Future studies should assess the impact of starch substitution on sensory characteristics, shelf-life stability, and patient satisfaction. Full article