Search Results (713)

Obstructive sleep apnea (OSA) could increase pulmonary artery pressure. However, the clinical consequences vary, mainly depending on comorbidities. Patients with pulmonary hypertension associated with lung diseases (World Health Organization (WHO) Group 3 pulmonary hypertension) are particularly vulnerable increases in pulmonary artery pressure. Managing pulmonary hypertension in this specific patient population presents a considerable challenge. While positive airway pressure therapy for OSA has shown promise in improving pulmonary hemodynamics in patients with obesity hypoventilation syndrome and chronic obstructive pulmonary disease, evidence is lacking for similar improvements in those with other pulmonary diseases and hypoventilation disorders. Furthermore, pulmonary-artery-specific therapies may carry a risk of clinical worsening in this group. Weight management and new pharmacotherapy have together emerged as a crucial intervention, demonstrating benefits for both OSA and pulmonary hemodynamics. We reviewed key studies that provide insights into the influence of OSA on WHO Group 3 pulmonary hypertension and the clinical management of both conditions. Full article

Background: There is a high demand for long-term postharvest storage of valuable perishables with high-quality preservation and minimal product loss due to decay and physiological disorders. Postharvest low-pressure storage (LPS) provides a viable option for many fruits. While recent studies have presented the details of technology, this pilot study presents the metabolomics changes due to the hypobaric storage of pomegranates as a model system. Methods: Nuclear magnetic resonance (NMR)-based metabolomics studies were performed on pomegranate fruit tissues, comparing fruit stored under LPS conditions versus the traditional storage system, with modified atmosphere packaging (MAP) as the control. The metabolomic changes in the exocarp, mesocarp, and arils were measured using ¹H NMR spectroscopy, and the results were analyzed using multivariate statistics. Results: Distinguishable differences were noted between the MAP and LPS conditions in fruit quality attributes and metabolite profiles. Sucrose levels in the aril, mesocarp, and exocarp samples were higher under LPS, while sucrose levels were reduced in MAP. In addition, alanine levels were more abundant in the mesocarp and exocarp samples, and ethanol concentration decreased in the exocarp samples, albeit less significantly. Conclusions: This pilot investigation shows the potential for using NMR as a valuable assessment tool for monitoring the performance of viable long-term storage conditions in horticultural commodities. Full article

A novel brain positron emission tomography (PET) radioligand, [¹¹C]HY-2-15, has potential for imaging alpha-synuclein aggregations in multiple system atrophy and misfolded tau proteins in tauopathies, based on its high binding affinity in disease brain tissue homogenates. Here, we demonstrate that [³H]HY-2-15 has the capability to bind to aggregated alpha-synuclein in multiple system atrophy brain and tau aggregations in progressive supranuclear palsy and corticobasal degeneration brain tissues via in vitro autoradiography study. A first-in-human pilot multicenter clinical study recruited a total of 10 subjects including healthy controls and patients with Parkinson’s disease, multiple system atrophy, or progressive supranuclear palsy. The study revealed that [¹¹C]HY-2-15 has a relatively higher specific uptake in the pallidum and midbrain of patients with progressive supranuclear palsy. Total-body scans performed on the PennPET Explorer showed the radiotracer was cleared by renal excretion. However, the rapid metabolism and low brain uptake resulted in a limited signal of [¹¹C]HY-2-15 in brain. Full article

The accurate monitoring of waterbird abundance and their habitat preferences is essential for effective ecological management and conservation planning in aquatic ecosystems. This study explores the efficacy of unmanned aerial vehicle (UAV)-based high-resolution orthomosaics for waterbird monitoring and mapping along the Lieve Canal, Belgium. We systematically classified habitats into residential, industrial, riparian tree, and herbaceous vegetation zones, examining their influence on the spatial distribution of three focal waterbird species: Eurasian coot (Fulica atra), common moorhen (Gallinula chloropus), and wild duck (Anas platyrhynchos). Herbaceous vegetation zones consistently supported the highest waterbird densities, attributed to abundant nesting substrates and minimal human disturbance. UAV-based waterbird counts correlated strongly with ground-based surveys (R² = 0.668), though species-specific detectability varied significantly due to morphological visibility and ecological behaviors. Detection accuracy was highest for coots, intermediate for ducks, and lowest for moorhens, highlighting the crucial role of image resolution ground sampling distance (GSD) in aerial monitoring. Operational challenges, including image occlusion and habitat complexity, underline the need for tailored survey protocols and advanced sensing techniques. Our findings demonstrate that UAV imagery provides a reliable and scalable method for monitoring waterbird habitats, offering critical insights for biodiversity conservation and sustainable management practices in aquatic landscapes. Full article

Strategies to interpret short-wave infrared hyperspectral images of rocks involve the application of analysis and classification steps that guide the extraction of geological and mineralogical information with the aim of creating mineral maps. Pre-existing strategies often rely on the use of statistical measures between reference and image spectra that are scene dependent. Therefore, classification thresholds based on statistical measures to create mineral maps are also scene dependent. This is problematic because thresholds must be adjusted between images to produce mineral maps of the same accuracy. We developed an innovative, knowledge-based strategy to perform mineralogical analyses and create classifications that overcome this problem by using physics-based wavelength positions of absorption features that are invariant between scenes as the main sources of mineral information. The strategy to interpret short-wave infrared hyperspectral images of rocks is implemented using the open source Hyperspectral Python package (HypPy) and demonstrated on a series of hyperspectral images of hydrothermally altered rock samples. The results show how expert knowledge can be embedded into a standardized processing chain to develop reproducible mineral maps without relying on statistical matching criteria. Full article

Youth Mental Health First Aid (YMHFA) is a widely adopted professional development tool that helps educators across the United States improve their mental health literacy. Data from a pretest/posttest evaluation of the YMHFA training delivered at five schools are used to explore whether various YMHFA outcomes differ for teachers who have and have not received previous mental health training. Specifically, the current study compares scores on confidence, knowledge, negative attitudes, and intentions to intervene prior to completing the YMHFA program (i.e., at baseline), the rate of change in each measure, and satisfaction with the training across teachers with and without previous mental health prevention training. Our findings showed that teachers with previous training scored higher on confidence, mental health knowledge, and intentions to intervene at baseline and experienced different patterns of change after completion of the YMHFA training program, compared to teachers without prior training. Negative attitudes and training satisfaction did not reveal differences across training experiences. Study findings offer important program and policy implications about teachers’ training experiences, as well as the value of implementing YMHFA as a universal training in educational settings. Full article

Background/Objectives: The COVID-19 pandemic introduced various stressors for families, including changes to daily routines, work, and schooling. Studies have linked these stressors to increased mental health challenges for parents and children. Sleep difficulties were also common during the pandemic, with some children and parents experiencing poorer sleep quality and shorter sleep duration. However, it remains unclear whether the effects of COVID-19 stress on mental health challenges are explained, at least in part, by effects of COVID-19 stress on child and/or parent sleep challenges. This study examined the impacts of COVID-19 stress on sleep and, in turn, mental health difficulties in school-aged children and their parents in Canada and the United States. Methods: Parents (N = 961) completed validated measures of COVID-19 stress, and of their own and their child’s sleep and mental health. Path analyses tested direct and indirect effects of COVID-19 stress on mental health outcomes with sleep problems as the potential mediator. Results: Child sleep problems partially mediated COVID-19 stress effects on both parent (β = 0.33) and child (β = 0.20) mental health difficulties, while parent sleep problems contributed significantly but to a lesser degree (parent mental health: β = 0.07; child mental health: β = 0.03). There also remained significant direct effects of COVID-19 stress on both child and parent mental health difficulties that were not mediated through sleep difficulties. Conclusions: Our findings underscore the interconnected nature of sleep and mental health, demonstrating that stress-related disruptions in sleep (particularly children’s sleep) can exacerbate mental health difficulties for both parents and children during crises like the COVID-19 pandemic. Full article

The fibrillary aggregation of α-synuclein is a hallmark of Parkinson’s disease (PD) and a potential target for diagnostics and therapeutics. Although substantial effort has been devoted to the development of positron emission tomography (PET) probes for detecting α-synuclein aggregates, no clinically suitable tracer has been reported. The design and synthesis of 43 new N-(6-methoxypyridin-3-yl)quinolin-2-amine derivatives and an evaluation of their α-synuclein binding affinity is reported here. Compounds 7f, 7j, and 8i exhibited high affinity for α-synuclein and were selected for ¹¹C, ¹⁸F, ¹²⁵I, or ³H radiolabeling. A photoaffinity variant, TZ-CLX, structurally related to 7j and 8i, demonstrated preferential binding to the C-terminal region of α-synuclein fibrils. PET brain imaging studies using [¹¹C]7f, [¹⁸F]7j, and [¹¹C]8i in non-human primates indicated that these three α-synuclein PET tracers penetrated the blood–brain barrier. Both [¹¹C]7f and [¹⁸F]7j showed more favorable brain washout pharmacokinetics than [¹¹C]8i. In vitro binding assays showed that [¹²⁵I]8i is a very potent α-synuclein radioligand, with K_d values of 5 nM for both PD brain tissues and LBD-amplified fibrils; it is also selective for PD tissues versus AD or control tissues. These results strongly suggest that the PET probes based on the N-(6-methoxypyridin-3-yl)quinoline-2-amine scaffold have potential utility in detecting α-synuclein aggregates in vivo. Full article

Monitoring the peak junction hotspot temperature in IGBT modules is critical for ensuring the reliability of high-power industrial multilevel inverters, particularly when operating under extreme thermal conditions, such as in traction applications. This study presents a comprehensive chip-level analytical loss and thermal model for estimation of the peak junction hotspot temperature in a three-level T-type neutral-point-clamped (TNPC) IGBT module. The developed model includes a detailed analytical assessment of conduction and switching losses, along with transient thermal network modeling, based on the actual electrical and thermal characteristics of the IGBT module. Additionally, a hybrid thermal–electrical stress experimental setup, designed to replicate real operating conditions, was implemented for a balanced three-phase inverter circuit utilizing a Semikron three-level IGBT module, with testing currents reaching 100 A and a critical case temperature of 125 °C. The analytically estimated module losses and peak junction hotspot temperatures were validated through direct experimental measurements. Furthermore, thermal simulations were conducted with Semikron’s SemiSel benchmark tool to cross-validate the accuracy of the thermo-electrical model. The outcomes show a relative estimation error of less than 1% when compared to experimental data and approximately 1.15% for the analytical model. These findings confirm the model’s accuracy and enhance the reliability evaluation of TNPC-IGBT modules in extreme thermal environments. Full article

Background/Objectives: We examined the in vivo effects of successive treatments with the histone deacetylase (HDAC) inhibitor romidepsin in patients with cutaneous T-cell lymphoma (CTCL), using changes in gene expression in peripheral blood mononuclear cells (PBMCs). Methods: Exploiting data from a highly responsive CTCL patient through 12 months of treatment, we identified a malignant cell predictor (MCP), a gene signature associated with the diminishing numbers of circulating malignant cells. Results: The MCP was successfully validated in the patient’s relapse sample 9 months after treatment was terminated and via an independent set of CTCL patient samples. Conclusions: The MCP set of genes contained novel CTCL markers, including membrane-associated proteins not normally expressed in lymphocytes. A subclass of those markers was also detectable in residual malignant cells undetected by flow cytometry in remission samples from a patient who relapsed 10 months later. We identified a subset of transcriptional regulators, miRNAs and methylation patterns associated with the effect of progressive treatments revealing potential mechanisms of transcriptional dysregulation and functional effects in the malignant cells. We demonstrate a role for transcriptional activator HLF, over-expressed in malignant cells, and downregulated transcriptional-suppressor and immune-modulator NFIL3, as regulators of CTCL-specific genes. Full article

Serious enteric disease caused by seven species of Eimeira continues to cause significant economic damage to the poultry industry. E. acervulina is one of the most widespread strains in farms and has a significant impact on chicken weight loss. Currently, the use of anticoccidial agents to suppress the occurrence of coccidiosis in farms is considerably restricted due to public health and environmental pollution issues. It is important to understand the protective immunity of the host against Eimeria infections with regard to natural products that could be used as alternatives to anticoccidial agents. Berberine chloride is known for its various biological functions, including its anti-parasite activity. However, its impact on intestinal morphology and immune-related activity in broilers infected with Eimeria still remains unclear. The aim of this study is to evaluate the anticoccidial effects of a berberine-based diet in broilers infected with E. acervulina and to monitor the host immune phenomenon using transcriptomic analysis. Administration of berberine to chickens infected with E. acervulina significantly reduced fecal oocyst production and intestinal lesion scores, and increased duodenal villus height, indicating anticoccidial activity and positive effects on intestinal morphology. Transcriptomic analysis of chickens infected with E. acervulina generally observed the down-regulation of metabolism-related genes and the up-regulation of cell integrity-related genes at day 4 post-infection. At day 6 post-infection, an increase in immune-related genes and cellular-homeostasis-related genes was generally observed. Berberine-treated and E. acervulina-infected chickens showed cytokine-cytokine receptor interaction in the second term in a Kyoto Encyclopedia of Genes and Genomes pathway analysis at day 4 post-infection, but not in chickens infected with E. acervulina alone, suggesting host immune changes induced by berberine. These results suggest that berberine, which exhibits anticoccidial effects, may have therapeutic and/or prophylactic potential in protecting the host from infectious and economic-loss-causing diseases, such as Eimeria infection. Full article

Background/Objectives: Naegleria fowleri is a free-living amoeba that invades brain tissues causing fatal primary amoebic meningoencephalitis (PAM). An effective and tolerable therapeutic agent is still lacking. Methods: A series of conformationally restricted analogs of miltefosine with varied restriction positions, stereochemical configuration and lengths of alkyl chain was investigated to discover more effective and less toxic agents than miltefosine. Results: Among tested compounds, derivatives 2a, 3b and 3d featuring 1,2- or 2,3-positional restriction with trans-configuration and tridecyl or behenyl alkyl chains were discovered as more potent and less cytotoxic agents. Compounds 2a, 3b and 3d elicited 3.49-, 3.58- and 6.03-fold relative potencies to miltefosine and 7.53, 3.90 and 3.49 selectivity indices, respectively. Furthermore, compounds 2a and 3b showed IC₉₀ values for N. fowleri lower than CC₅₀ against glial C6 cells. Compounds 2a, 3b and 3d induced morphological changes and programmed cell death of N. fowleri via the apoptosis-like pathway. The induced death of N. fowleri involved DNA fragmentation along with the loss of mitochondrial membrane potential. Conclusions: The current research presents compounds 2a and 3b as more potent, selective and effective agents than miltefosine against N. fowleri for further development. Full article

Two-dimensional imaging is still commonly used in dentistry, but does not provide the three-dimensional information often required for the accurate assessment of dental structures. Photon-counting computed tomography (PCCT), a new three-dimensional modality mainly used in general medicine, has shown promising potential for dental applications. With growing digitalization and cross-disciplinary integration, using PCCT data from other medical fields is becoming increasingly relevant. Conventional CT scans, such as those of the cervical spine, have so far lacked the resolution to reliably evaluate dental structures or implants. This study evaluates the diagnostic utility of PCCT for visualizing peri-implant structures in routine clinical photon-counting CT acquisitions and assesses the influence of metal artifact reduction (MAR) algorithms on image quality. Ten dental implants were retrospectively included in this IRB-approved study. Standard PCCT scans were reconstructed at multiple keV levels with and without MAR. Quantitative image analysis was performed with respect to contrast and image noise. Qualitative evaluation of peri-implant tissues, implant shoulder, and apex was performed independently by two experienced dental professionals using a five-point Likert scale. Inter-reader agreement was measured using intraclass correlation coefficients (ICCs). PCCT enabled high-resolution imaging of all peri-implant regions with excellent inter-reader agreement (ICC > 0.75 for all structures). Non-MAR reconstructions consistently outperformed MAR reconstructions across all evaluated regions. MAR led to reduced clarity, particularly in immediate peri-implant areas, without significant benefit from energy level adjustments. All imaging protocols were deemed diagnostically acceptable. This is the first in vivo study demonstrating the feasibility of opportunistic dental diagnostics using PCCT in a clinical setting. While MAR reduces peripheral artifacts, it adversely affects image clarity near implants. PCCT offers excellent image quality for peri-implant assessments and enables incidental detection of dental pathologies without additional radiation exposure. PCCT opens new possibilities for opportunistic, three-dimensional dental diagnostics during non-dental CT scans, potentially enabling earlier detection of clinically significant pathologies. Full article

Background/Objectives: While anterior lumbar interbody fusion (ALIF) is a well-established treatment for degenerative lumbar spine pathology, the timing and pace of postoperative recovery remain poorly defined. Understanding these temporal trends is clinically important for setting patient expectations and optimizing postoperative care. Methods: This retrospective single-institution study evaluated functional recovery in patients undergoing primary, single-level stand-alone (SA) ALIF, or with percutaneous posterior instrumentation (PI). Patient-reported outcome measures (PROMs), including the Oswestry Disability Index (ODI), the Visual Analog Scale (VAS) for back and leg pain, and the SF-12 Physical Component Score (PCS), were assessed preoperatively and at 2 weeks, 6 weeks, 3 months, 6 months, 1 year, and 2 years postoperatively. Achievement of minimum clinically important difference (MCID), global rating change (GRC), and return-to-activity milestones were also analyzed. Results: A total of 143 patients were included (90 SA; 53 PI). PROMs showed significant improvement through 1 year. VAS-back improved by 2 weeks, while ODI and SF12 PCS initially worsened but improved after 6 weeks. By 6 months, over half of the cohort achieved MCID, with continued gains through 1 year. Most patients returned to driving and work, and over 90% discontinued narcotics. Recovery trajectories were comparable between groups, despite early delays in the instrumented cohort. Conclusions: These findings provide time-specific recovery benchmarks that can guide surgical decision-making, patient education, and expectations around functional milestones. Full article

Rapid suburbanization can alter catchment flow regime and increase stormwater runoff, posing threats to sensitive ecosystems. Applications of Nature-based Solutions (NbS) have increasingly been adopted as part of integrated water management efforts to tackle the hydrological impact of urbanization with co-benefits for improved urban resilience, sustainability, and community well-being. However, the implementation of NbS can be hindered by gaps in performance assessment. This paper introduces a physically based dynamic modeling approach to assess the performance of a nature-based storage facility designed to capture excess runoff from an urbanizing catchment (Armstrong Creek catchment) in Geelong, Australia. The study adopts a numerical modelling approach, supported by extensive field monitoring of water levels over a 2.5-year period. The model provides a decision support tool for Geelong local government in managing stormwater runoff to protect Lake Connewarre, a Ramsar-listed wetland under the Port Phillip Bay (Western Shoreline) and Bellarine Peninsula. Runoff is currently managed via a set of operating rules governing gate operations that prevents flows into the ecological sensitive downstream waterbody from December to April (drier periods in summer and most of autumn). Comparison with observed water level data at three monitoring stations for a continuous simulation period of May 2022 to October 2024 demonstrates satisfactory to excellent model performance (NSE: 0.55–0.79, R²: 0.80–0.89, ISE rating: excellent). Between 1670 × 10³ m³ and 2770 × 10³ m³ of runoff was intercepted by the nature-based storage facility, representing a 56–70% reduction in stormwater discharge into Lake Connewarre. Our model development underscores the importance of understanding and incorporating user interventions (gate operations and emergency pumping) from the standard operation plan to better manage catchment runoff. As revealed by the seasonal flow analysis for consecutive years, adaptive runoff management practices, capable of responding to rainfall variability, should be incorporated. Full article