Sperm Biotechnology in Animals Reproduction—2nd Edition

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Reproduction and Obstetrics".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 449

Special Issue Editors


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Guest Editor
Faculty of Veterinary Sciences, Department of Animal Medicine and Surgery, University of Murcia, 30071 Murcia, Spain
Interests: animal reproduction; reproductive biotechnologies related to spermatozoa and embryos
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Guest Editor
Department of Agriculture, School of Agricultural Sciences, University of Western Macedonia, 53100 Florina, Greece
Interests: animals reproduction; artificial insemination; IVF; semen technology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Advances in sperm technology and artificial insemination with liquid-/cryo-preserved or sexed semen, depending on species, have been widely applied in practice and have facilitated, among other things, the distribution of genetically strong animals worldwide. However, there are many points related to sperm biotechnological techniques that require further investigation. The improvement of sperm survival during liquid- or cryo-preservation and sex-sorting processes or the increase in the recovery rate of good quality sperm during sperm selection techniques is a requirement that is always related to high fertility rates (either in vivo or in vitro). In this field, research has focused on the extender’s composition, the cryo-preservation protocols, and the discovery of agents or mechanisms that could improve sperm fertilizing capacity. Molecular techniques have already provided a new perspective in reproductive medicine that could further enhance our knowledge and yield novel insights into diagnosis by introducing new fertility markers. Modern diagnostic techniques for sperm quality and function evaluation could be applied for diagnosis in clinical practice or the prediction of animal fertility. New methods for sperm selection and handling in reproductive technologies could also increase the recovery rate of good quality sperm.

We are pleased to invite you to contribute to this Special Issue, which aims to publish original research articles or reviews on “Sperm biotechnology and its applications”.

We invite researchers to contribute their recent findings, mainly focusing on, but not limited to, the following scientific topics:

  • Semen extenders;
  • Sperm technology;
  • Sperm sorting methods for assisted reproductive protocols;
  • New fertility markers for diagnosis;
  • Modern techniques for prediction of animal fertility;
  • Artificial insemination in domestic animals.

Dr. Inmaculada Parrilla Riera
Dr. Athina Basioura
Dr. Junwei Li
Guest Editors

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • spermatozoa
  • fertility
  • sperm fertilizing capacity
  • sperm sorting
  • assisted reproduction technologies
  • sperm biotechnological applications

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Published Papers (1 paper)

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Research

11 pages, 600 KiB  
Article
Semen Evaluation from Dominant Males of the Viviparous Mexican Lizard Sceloporus torquatus, Wiegmann, 1828 (Sauria: Phrynosomatidae)
by Martín Martínez-Torres, Uriel Ángel Sánchez-Rivera, Alfredo Medrano, Enrique Othón Hernández-Gónzalez, Rodrigo Dávila-Govantes, Yabín Josué Castro-Camacho and Norma Berenice Cruz-Cano
Vet. Sci. 2025, 12(4), 363; https://doi.org/10.3390/vetsci12040363 - 13 Apr 2025
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Abstract
While most lizard species are polygamous, dominant males typically have greater access to females and copulate more frequently than submissive ones. Several studies suggest that applying assisted reproduction techniques to this taxon could be an effective tool to enhance conservation programs. Therefore, the [...] Read more.
While most lizard species are polygamous, dominant males typically have greater access to females and copulate more frequently than submissive ones. Several studies suggest that applying assisted reproduction techniques to this taxon could be an effective tool to enhance conservation programs. Therefore, the proper selection of males for assisted breeding programs is crucial for their overall success. In this study, we report for the first time data on semen and sperm quality from dominant males of the Mexican lizard Sceloporus torquatus using techniques commonly employed in assessing mammalian fertility. The semen and sperm characteristics were as follows: volume, 14.0 µL; sperm concentration, 125.7 × 106 cells/mL; wave motion, 2.7; total motility, 87.8%; sperm viability, 89.0%; normal morphology, 88.8%; plasma membrane integrity, 87.7%; low plasma membrane fluidity, 94.9%; capacitation status: uncapacitated (F), 90.5%; capacitated (B), 7.2%; sperm with acrosome reaction (AR), 2.3%; and, acrosome integrity, 88.8%. These results suggest that, according to mammalian fertility parameters, dominant males of S. torquatus are fertile. This series of tests provides a valuable tool for conducting a comprehensive analysis of the functional changes that may occur in sperm handling during assisted processes, and that may contribute—along with the proper selection of individuals—to reproductive success. Full article
(This article belongs to the Special Issue Sperm Biotechnology in Animals Reproduction—2nd Edition)
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