Advances in Biomass Pretreatment

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Environmental and Green Processes".

Deadline for manuscript submissions: closed (10 March 2023) | Viewed by 4931

Special Issue Editors


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Guest Editor
Department of Biotechnology, Faculty of Biological Sciences, Kazimierz Wielki University, 85-671 Bydgoszcz, Poland
Interests: optimization of bioethanol production technology from lignocellulosic biomass and starchy raw materials (improvement of the ethanol productivity, costs reduction, energy saving); improvement of the technological methods used for the enzymatic degradation of polysaccharides; impact of raw materials contamination and quality on fermentation yield and formation of alcoholic fermentation volatile by-products (quality of raw spirit and alcoholic beverages); screening and selection strategies of filamentous fungi degrading cellulosic biomass
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Guest Editor
Department of Biotechnology, Faculty of Biological Sciences, Kazimierz Wielki University, 85-671 Bydgoszcz, Poland
Interests: bioethanol; biomass pretreatment; fermentation technology; yeast; cellulose hydrolysis; microwave-assisted pretreatment; biosynthesis; biotransformation; biofuels production; biocatalysis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue of Processes entitled "Advances in Biomass Pretreatment" includes the publication of original research articles and reviews of the latest developments in lignocellulosic biomass pretreatment and decomposition. One of the necessary conditions for the use of lignocellulosic biomass as a raw material in microbial biosynthesis processes is that it is an effective pretreatment. This process reduces the cellulose crystallinity, relaxes the lignocellulose structure by partially hydrolyzing hemicellulose, and increases the efficacy of the subsequent enzymatic hydrolysis stage. The expected effect of a successful pretreatment is plant biomass being more susceptible to enzymatic hydrolysis and a reduced amount of fermentation inhibitors (mainly organic acids and sugar dehydration products). Pretreatment methods include physical techniques (grinding, extrusion, ultrasounds, and microwaves) and chemical methods (use of concentrated acids and bases, ionic liquids, ozonolysis, and Organosolv). However, the most effective are physico-chemical methods, which include pretreatment in acidic environments under elevated temperature and pressure, steam explosion, ammonia fiber explosion (AFEX), CO2 explosion pretreatment, and wet oxidation. This Special Issue will also include articles describing the industrial application of lignocellulosic biomass pretreatment methods.

Dr. Grzegorz Kłosowski
Dr. Dawid Mikulski
Guest Editors

Manuscript Submission Information

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Published Papers (3 papers)

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Research

14 pages, 4116 KiB  
Article
Enhanced Enzymatic Hydrolysis of Wheat Straw to Improve Reducing Sugar Yield by Novel Method under Mild Conditions
by Xuyang Zhao, Lihua Han, Xuejiao Ma, Xiaoran Sun and Zheng Zhao
Processes 2023, 11(3), 898; https://doi.org/10.3390/pr11030898 - 16 Mar 2023
Cited by 4 | Viewed by 1439
Abstract
Wheat straw is a suitable source material for bioethanol production. Removing lignin and hemicellulose in wheat straw to improve enzymatic hydrolysis efficiency is essential because of its complex structure. Deep eutectic solvents (DESs) have become substitutes for ionic liquids (ILs), with the characteristics [...] Read more.
Wheat straw is a suitable source material for bioethanol production. Removing lignin and hemicellulose in wheat straw to improve enzymatic hydrolysis efficiency is essential because of its complex structure. Deep eutectic solvents (DESs) have become substitutes for ionic liquids (ILs), with the characteristics of good biocompatibility, simple synthesis procedure and low cost. However, the process of removing lignin and hemicellulose using present DESs requires a high operation temperature or long operation time. Therefore, we studied a novel method under mild conditions for screening a series of novel DESs based on an inorganic base to remove lignin and hemicellulose in wheat straw. In this work, the effect of DES type, the pH of the DESs, the operation temperature and operation time for enhancing enzymatic hydrolysis, and the crystal structure and the chemical structure and surface morphology of wheat straw were investigated. In particular, Na:EG exhibited the most excellent solubility for wheat straw under mild conditions, removing 80.6% lignin and 78.5% hemicellulose, while reserving 87.4% cellulose at 90 °C for 5 h, resulting in 81.6% reducing sugar produced during hydrolysis for 72 h. Furthermore, XRD, FT-IR and SEM analysis verified the lignin and hemicellulose removal. Hence, DESs based on an inorganic base used for removing lignin and hemicellulose will enhance enzymatic hydrolysis, and thus promote the industrial application of wheat straw to produce bioethanol. Full article
(This article belongs to the Special Issue Advances in Biomass Pretreatment)
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14 pages, 2479 KiB  
Article
Two-Stage Pretreatment of Jerusalem Artichoke Stalks with Wastewater Recycling and Lignin Recovery for the Biorefinery of Lignocellulosic Biomass
by Yudian Chen, Nian Peng, Yushan Gao, Qian Li, Zancheng Wang, Bo Yao and Yonghao Li
Processes 2023, 11(1), 127; https://doi.org/10.3390/pr11010127 - 1 Jan 2023
Cited by 3 | Viewed by 1748
Abstract
Jerusalem artichoke (Helianthus tuberosus L.) is emerging as one of the energy plants considered for biofuel production. Alkali and alkali-involved pretreatment methods have been widely used for the bioconversion of cellulosic materials due to their high sugar yield and low inhibitor release. [...] Read more.
Jerusalem artichoke (Helianthus tuberosus L.) is emerging as one of the energy plants considered for biofuel production. Alkali and alkali-involved pretreatment methods have been widely used for the bioconversion of cellulosic materials due to their high sugar yield and low inhibitor release. However, the recovery and treatment of wastewater (black liquor) have been poorly studied. Here, we present a novel two-stage pretreatment process design for recycling black liquor. Jerusalem artichoke stalk (JAS) was first treated with 2% (w/v) NaOH, after which lignin was recovered by H2SO4 at pH 2.0 from the black liquor. The recycled solutions were subsequently used to treat the NaOH-pretreated JAS for the second time to dissolve hemicellulose. CO-pretreated JAS, hydrolysates, and acid-insoluble lignin were obtained after the above-mentioned two-stage pretreatment. A reducing sugar yield of 809.98 mg/g Co-pretreated JAS was achieved after 48 h at 5% substrate concentration using a cellulase dosage of 25 FPU/g substrate. In addition, hydrolysates containing xylose and acid-insoluble lignin were obtained as byproducts. The pretreatment strategy described here using alkali and acid combined with wastewater recycling provides an alternative approach for cellulosic biorefinery. Full article
(This article belongs to the Special Issue Advances in Biomass Pretreatment)
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12 pages, 3179 KiB  
Article
A Novel Method to Detoxify Steam-Exploded Biomass and Produce a Substrate for Biorefinery
by Francesco Zimbardi, Egidio Viola, Giuseppe Arcieri, Vito Valerio and Massimo Carnevale
Processes 2022, 10(12), 2611; https://doi.org/10.3390/pr10122611 - 6 Dec 2022
Cited by 1 | Viewed by 1201
Abstract
Pre-treatments at relatively high temperatures (range 160 °C–220 °C) are currently used to transform lignocellulosics into biofuels and chemicals. In this step, several molecules with an inhibitory effect in the subsequent fermentation processes are generated. These inhibitors include low-molecular-weight molecules and lignin fragments [...] Read more.
Pre-treatments at relatively high temperatures (range 160 °C–220 °C) are currently used to transform lignocellulosics into biofuels and chemicals. In this step, several molecules with an inhibitory effect in the subsequent fermentation processes are generated. These inhibitors include low-molecular-weight molecules and lignin fragments that can be removed by water washing. However, this procedure also removes valuable soluble carbohydrates which are then difficult to recover from the diluted stream. In this work, a new method to detoxify steam-exploded substrates is reported. The procedure is based on the evaporation of low-weight acids and aldehydes, which leaves all the sugars in the solid matrix, while the cellulose hornification (an irreversible modification of the cellulose fibres that depresses the saccharification yield) is prevented by adding steam to the hot fluidizing flow stream. Two systems were tested: a 0.1 kg/batch oscillating fluidized bed and a continuous fluidized bed dryer operating downstream of a steam explosion plant with a treatment capacity of 150 kg/h. The detoxified substrates were subjected to enzymatic hydrolysis and fermentation to obtain bioethanol, with a yield that was 14% higher than that obtained from substrates detoxified with conventional methods of drying or washing. Full article
(This article belongs to the Special Issue Advances in Biomass Pretreatment)
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