Plant Cell Differentiation during Embryogenesis and Protoplast Cultures

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Physiology and Metabolism".

Deadline for manuscript submissions: closed (10 October 2022) | Viewed by 3214

Special Issue Editor


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Guest Editor
Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic
Interests: cell differentiation; plant biotechnology; in vitro cultures

Special Issue Information

Dear Colleagues,

Plant cell differentiation is a fascinating aspect of plant developmental biology. A comprehensive understanding of this process could improve the strategies  available for biotechnology research and enhance the application of biotechnological techniques in plant modification and breeding processes. Several biotechnological approaches strongly concern cell differentiation, such as plant embryogenesis and protoplast cultures. Plant embryogenesis does not only include zygotic embryogenesis in planta or in vitro, but also somatic embryogenesis and, in part, androgenesis. These abovementioned developmental processes are characterized at different biological levels: from cell and tissue morphology, gene activities and transcriptional profiles to epigenetic changes and growth regulator content or transport. This Special Issue of Plants will highlight the most recent advances in plant cell differentiation, taking into account a wide spectrum of approaches to plant embryogenesis and protoplast cultures in an effort to improve plant development.   

Dr. Vladan Ondřej
Guest Editor

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Keywords

  • cell differentiation
  • plant embryogenesis
  • protoplast cultures

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Published Papers (1 paper)

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Research

12 pages, 4383 KiB  
Article
Expression of Genes Involved in ABA and Auxin Metabolism and LEA Gene during Embryogenesis in Hemp
by Daniel Král, Josef Baltazar Šenkyřík and Vladan Ondřej
Plants 2022, 11(21), 2995; https://doi.org/10.3390/plants11212995 - 7 Nov 2022
Cited by 4 | Viewed by 2776
Abstract
The level of phytohormones such as abscisic acid (ABA) and auxins (Aux) changes dynamically during embryogenesis. Knowledge of the transcriptional activity of the genes of their metabolic pathways is essential for a deeper understanding of embryogenesis itself; however, it could also help breeding [...] Read more.
The level of phytohormones such as abscisic acid (ABA) and auxins (Aux) changes dynamically during embryogenesis. Knowledge of the transcriptional activity of the genes of their metabolic pathways is essential for a deeper understanding of embryogenesis itself; however, it could also help breeding programs of important plants, such as Cannabis sativa, attractive for the pharmaceutical, textile, cosmetic, and food industries. This work aimed to find out how genes of metabolic pathways of Aux (IAA-1, IAA-2, X15-1, X15-2) and ABA (PP2C-1) alongside one member of the LEA gene family (CanLea34) are expressed in embryos depending on the developmental stage and the embryo cultivation in vitro. Walking stick (WS) and mature (M) cultivated and uncultivated embryos of C. sativa cultivars ‘KC Dora’ and ‘USO 31’ were analyzed. The RT-qPCR results indicated that for the development of immature (VH) embryos, the genes (IAA-1, IAA-2) are likely to be fundamental. Only an increased expression of the CanLea34 gene was characteristic of the fully maturated (M) embryos. In addition, this feature was significantly increased by cultivation. In conclusion, the cultivation led to the upsurge of expression of all studied genes. Full article
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