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Analytical Chemistry in Asia, 2nd Edition
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Analytical Chemistry in Asia, 2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1114

Special Issue Editor

Dr. Makoto Tsunoda

E-Mail Website
Guest Editor
Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan
Interests: HPLC; fluorescence; chemiluminescence; microfluidics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In Asia, many researchers are devoted to the dissemination of new and original knowledge in all branches of analytical chemistry. In order to assemble these findings, a Special Issue entitled “Analytical Chemistry in Asia, 2nd Edition” is being launched. This Special Issue will present a high-quality collection comprising work from scientists in Asian countries. Both original research articles and comprehensive review papers are welcome.

This Special Issue will discuss new knowledge and cutting-edge developments in the field of analytical chemistry, with the aim of expanding the current body of knowledge. This issue is intended as a forum for the exchange of research findings and innovative ideas in the field.

Dr. Makoto Tsunoda
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • analytical chemistry
  • Asia
  • HPLC
  • sample preparation
  • bioanalysis
  • mass spectrometry

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Related Special Issue

Published Papers (4 papers)

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Research

13 pages, 1243 KiB  
Article
Identification of Chemical Components in Three Types of Rose Essential Oils Based on Gas Chromatography-Mass Spectrometry (GC-MS) and Chemometric Methods
by Min Xu, Jia Cai, Long Wang, Shunpeng Zhu, Yangxi Chen, Yuchen Chen, Jie Zhong, Jiaxin Li, Peng Hu and Qiang Ye
Molecules 2025, 30(9), 1974; https://doi.org/10.3390/molecules30091974 - 29 Apr 2025
Abstract
Currently, the main types of roses circulating in China include Jinbian Rose, Kushui Rose and Pingyin Rose. Each type of rose has slight differences in usage and efficacy. There are many varieties of roses, and the quality of rose essential oils varies greatly. [...] Read more.
Currently, the main types of roses circulating in China include Jinbian Rose, Kushui Rose and Pingyin Rose. Each type of rose has slight differences in usage and efficacy. There are many varieties of roses, and the quality of rose essential oils varies greatly. Almost no research has systematically studied the essential oils of various roses. In this experiment, three types of roses (Jinbian Rose, Kushui Rose, and Pingyin Rose) were selected as research subjects based on their efficacy and variety in the market. Essential oils were extracted from the three types of roses using hydrodistillation. Gas chromatography-mass spectrometry (GC-MS) was used to qualitatively analyze the volatile substances in the essential oils of different varieties of roses. The three types of rose essential oils were identified and differentiated using chemometric methods (including HCA, PCA, PLS-DA, and OPLS-DA). On the one hand, based on the GC-MS analysis results, 40, 48, and 40 volatile components were detected in Jinbian Rose, Kushui Rose, and Pingyin Rose, respectively. The chemical compositions were primarily dominated by macromolecular compounds such as long-chain alkanes, organic acids, and esters. On the other hand, eight markers with significant identification values were identified to distinguish among the three types of roses. In conclusion, based on GC-MS analysis and chemometric methods, this experiment distinguishes and identifies three types of roses from the perspective of essential oil components for developing an effective strategy for the identification of rose varieties. Full article
(This article belongs to the Special Issue Analytical Chemistry in Asia, 2nd Edition)
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13 pages, 5468 KiB  
Article
A Bifunctional Fluorescence Probe for the Detection of Hypochlorous Acid and Viscosity in Living Cells and Zebrafish
by Xin Zhang, Yanmei Si, Xinpeng Chen, Xuqing Nie, Yiheng Zhang, Li Lin and Yehao Yan
Molecules 2025, 30(7), 1531; https://doi.org/10.3390/molecules30071531 - 30 Mar 2025
Viewed by 272
Abstract
As two significant indicators in the microenvironment, hypochlorous acid and viscosity play important roles in multitudinous physiological metabolic processes. However, it is challenging to determine the dynamic levels of hypochlorous acid and viscosity in living cells and organisms because of the absence of [...] Read more.
As two significant indicators in the microenvironment, hypochlorous acid and viscosity play important roles in multitudinous physiological metabolic processes. However, it is challenging to determine the dynamic levels of hypochlorous acid and viscosity in living cells and organisms because of the absence of effective molecular tools that can simultaneously detect hypochlorous acid and viscosity in organisms. Herein, a molecular design strategy was presented to fabricate a single fluorescence probe that can simultaneously detect hypochlorous acid and viscosity by using two different emission channels. In JXR, TICT-based 4-(2-(5-(dimethylamino)thiophen-2-yl)vinyl)-1-methylpyridin-1-ium-iodide chromophore serves as energy acceptor in the FRET process and sensors for hypochlorous acid and viscosity. JXR showed a large Stokes shift, wide emission peak distance, high photostability, and low toxicity. JXR could detect hypochlorous acid and viscosity rapidly, sensitively, and selectively by emitting different fluorescence signals. Importantly, JXR was successfully applied to track the intracellular hypochlorous acid and viscosity in living cells. Meanwhile, the generation of endogenous hypochlorite in living cells can be observed by using JXR. Full article
(This article belongs to the Special Issue Analytical Chemistry in Asia, 2nd Edition)
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14 pages, 3376 KiB  
Article
One-Step Solvothermal Synthesis of Carbon Dots for Rapid and Accurate Determination of Hemin Content
by Yiaobo Zhang, Lin Liu, Jiahui He, Chengzhi Huang, Lei Zhan and Chunmei Li
Molecules 2025, 30(6), 1343; https://doi.org/10.3390/molecules30061343 - 17 Mar 2025
Viewed by 298
Abstract
The development of sensitive and specific methods for the high-quality analysis of hemin-related drugs is significant in the pharmaceutical field. In this work, a simple and rapid method based on the fluorescent properties of carbon dots (CDs) was established for the determination of [...] Read more.
The development of sensitive and specific methods for the high-quality analysis of hemin-related drugs is significant in the pharmaceutical field. In this work, a simple and rapid method based on the fluorescent properties of carbon dots (CDs) was established for the determination of hemin in drugs. By taking melamine and ethylenediamine as the reaction materials, the fluorescent CDs were synthesized by a one-step solvothermal method, which can be used for the determination of hemin in drugs by the fluorescent inner filter effect. The as-prepared fluorescent CDs with rich functional groups on the surface displayed good water solubility, strong salt resistance, robust pH stability, and photobleaching resistance. Most importantly, the fluorescent excitation wavelength of fluorescent CDs was very close to the absorption wavelength of hemin, providing the evidence for the fluorescent inner filter effect. When the hemin concentration was in the range of 0.01–1 μM, there was a good linear relationship between the hemin content with the fluorescence intensity of CDs. The linear regression equation was (1 − F/F0) = 0.0897c + 0.0124, with a correlation coefficient (R2) of 0.9982 and a detection limit of 9 nM. This assay was successfully used to determine the content of hemin in the tablet, which displayed 97.9–105.5% of the labelled amount, with a relative standard deviation of less than 3%. The developed fluorescence method for the detection of hemin content displays the advantages of accurate, rapid, and high sensitivity, which could prove to be a useful tool for the determination of hemin supplement tablets. Full article
(This article belongs to the Special Issue Analytical Chemistry in Asia, 2nd Edition)
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Graphical abstract

12 pages, 1949 KiB  
Communication
Quantitative Evaluation of Iron-Containing Proteins Bound to Mesoporous Silica Microspheres by Inductively Coupled Plasma Mass Spectrometry and Confocal Laser Raman Microscopy
by Shin-ichi Miyashita, Toshihiko Ogura, Shun-ichi Matsuura and Eriko Fukuda
Molecules 2025, 30(6), 1252; https://doi.org/10.3390/molecules30061252 - 11 Mar 2025
Viewed by 443
Abstract
Inductively coupled plasma mass spectrometry (ICP-MS) is important in the biological and biochemical fields as it can quantify trace elements. Confocal laser Raman microscopy (CLRM), a powerful tool for the compositional analysis of biological samples, organic materials, and inorganic materials, can be used [...] Read more.
Inductively coupled plasma mass spectrometry (ICP-MS) is important in the biological and biochemical fields as it can quantify trace elements. Confocal laser Raman microscopy (CLRM), a powerful tool for the compositional analysis of biological samples, organic materials, and inorganic materials, can be used to analyze samples in aqueous solutions. Despite their analytical strength, the quantitative evaluation of proteins bound to mesoporous silica (SiO2) microspheres, which are promising candidates for drug delivery systems and vaccine carriers, has not been sufficiently explored. Therefore, we investigated the applicability of ICP-MS and CLRM to quantify lactoferrin (LF), a widely studied iron-containing protein bound to mesoporous SiO2 microspheres (SBA24). The bound LF amount was measured using ICP-MS, selectively monitoring iron derived from LF as a marker element, and CLRM. The results were compared with those obtained using a conventional bulk analysis technique. The amounts and trends of the signal intensities obtained using ICP-MS and CLRM agreed with each other and with the bulk analysis results. Our findings demonstrate that ICP-MS and CLRM are applicable for the quantitative evaluation of iron-containing proteins bound to SBA24. These methods offer a reliable platform for the quantification of biomolecules on microparticles and provide valuable insights for biomedical research and quality control in related industries. Full article
(This article belongs to the Special Issue Analytical Chemistry in Asia, 2nd Edition)
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