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Recent Advances in Protein Synthesis

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Chemical Biology".

Deadline for manuscript submissions: 30 November 2024 | Viewed by 1593

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Guest Editor
Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), Universidad Autonoma de Madrid, Cantoblanco, Calle Nicolás Cabrera 9, CP. 28049 Madrid, Spain
Interests: protein engineering; chimeric proteins applications; protein production; modification of active centers for different industrial applications; proteins for hydrocarbon biosulfurization; synthetic proteins; design of matrices for protein purification; proteins and different industrial applications; multienzyme proteins
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Special Issue Information

Dear Colleagues, 

Recent advances in protein synthesis have revolutionized our understanding of cellular processes, enabling groundbreaking discoveries and the development of innovative technologies. Protein synthesis, the cellular mechanism by which amino acids are linked together to form proteins, is a fundamental process crucial for life. In recent years researchers have made significant strides in this field, with noteworthy achievements in both understanding the intricacies of protein synthesis and enhancing its applications.

Technological advancements, such as high-throughput methods and advanced imaging techniques, have allowed scientists to explore the dynamics of protein synthesis in unprecedented detail. The identification of new ribosomal components, the elucidation of intricate translation mechanisms, and the discovery of regulatory factors have expanded our knowledge of the complexity of protein synthesis at the molecular level.

In addition to fundamental research, recent innovations in synthetic biology and biotechnology have leveraged protein synthesis for various applications. These include the production of therapeutic proteins, the engineering of novel enzymes with enhanced functionalities, and the development of bio-based materials. The ability to precisely control and manipulate protein synthesis holds immense promise for fields ranging from medicine to industrial biotechnology.

Dr. Benevides C. Pessela
Guest Editor

Manuscript Submission Information

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Keywords

  • protein synthesis
  • synthetic biology
  • biotechnology
  • proteins
  • screening enzymes
  • enzymes

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Published Papers (1 paper)

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Research

13 pages, 1873 KiB  
Article
Cell-Free Protein Expression by a Reconstituted Transcription–Translation System Energized by Sugar Catabolism
by Gaku Sato, Shintaro Miyazawa, Nobuhide Doi and Kei Fujiwara
Molecules 2024, 29(13), 2956; https://doi.org/10.3390/molecules29132956 - 21 Jun 2024
Viewed by 1150
Abstract
Cooperation between catabolism and anabolism is crucial for maintaining homeostasis in living cells. The most fundamental systems for catabolism and anabolism are the glycolysis of sugars and the transcription–translation (TX-TL) of DNA, respectively. Despite their importance in living cells, the in vitro reconstitution [...] Read more.
Cooperation between catabolism and anabolism is crucial for maintaining homeostasis in living cells. The most fundamental systems for catabolism and anabolism are the glycolysis of sugars and the transcription–translation (TX-TL) of DNA, respectively. Despite their importance in living cells, the in vitro reconstitution of their cooperation through purified factors has not been achieved, which hinders the elucidation of the design principle in living cells. Here, we reconstituted glycolysis using sugars and integrated it with the PURE system, a commercial in vitro TX-TL kit composed of purified factors. By optimizing key parameters, such as glucokinase and initial phosphate concentrations, we determined suitable conditions for their cooperation. The optimized system showed protein synthesis at up to 33% of that of the original PURE system. We observed that ATP consumption in upstream glycolysis inhibits TX-TL and that this inhibition can be alleviated by the co-addition of glycolytic intermediates, such as glyceraldehyde 3-phosphate, with glucose. Moreover, the system developed here simultaneously synthesizes a subset of its own enzymes, that is, glycolytic enzymes, in a single test tube, which is a necessary step toward self-replication. As glycolysis and TX-TL provide building blocks for constructing cells, the integrated system can be a fundamental material for reconstituting living cells from purified factors. Full article
(This article belongs to the Special Issue Recent Advances in Protein Synthesis)
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