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New Development in Fermented Products—Third Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 1976

Special Issue Editors


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Guest Editor
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, Italy
Interests: antioxidant capacities; glucans; pectins; seed oils; citrus essential oils; fatty acids; fermented products; kefir kombucha
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende, Italy
Interests: antioxidant capacities; glucans; pectins; fermented products; kefir kombucha
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fermented foods and beverages were the first processed food products consumed by humans.

They are produced through the metabolic activity of microorganisms via two main methods, “wild or spontaneous fermentation” by microorganisms naturally present in the raw food matrices or processing environment (such products include kimchi and some fermented soy products) and “culture-dependent fermentation” performed via the addition of starter cultures (e.g., kefir and kombucha). There are several variables in the fermentation process, including microorganisms, nutritional ingredients, and environmental conditions, leading to different products.

Fermentation generates antimicrobial end-products such as organic acids, ethanol, and bacteriocins that may reduce the risk of contamination, improve the products’ shelf life, and confer new and desirable organoleptic characteristics completely unlike those of the starting material. Fermented foods are increasingly studied for their properties, beyond merely their preservation and sensory attributes.

Advantages of fermentation include the reduction in possible toxins and anti-nutrients; reduced content of fermentable carbohydrates, increasing the tolerance to products in patients with bowel disorders; and the microbial conversion of raw matrix compounds into biologically active metabolites exerting beneficial effects on human health.

The main objective of this Special Issue is to highlight the traditional and innovative technologies involved in the production of fermented foods and beverages. We welcome the submission of original research articles and reviews studying the aspects of fermentation processes, microbiological, technological and sensory aspects, shelf life, and health benefits of fermented products.

Dr. Alessia Fazio
Dr. Chiara La Torre
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • application of microorganisms to the production of fermented food
  • traditional and innovative fermentation technologies
  • natural health benefits of fermented foods
  • homemade and industrial fermented products
  • identification and production of novel starter cultures of commercial interest
  • microbial biotransformation and production of active metabolites
  • mechanisms of action of fermented food on the microbiota

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Published Papers (2 papers)

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Research

17 pages, 5442 KB  
Article
Solid-State Fermentation of Riceberry Rice with Mushroom Mycelium for Enhanced Beta-Glucan Production and Health Applications
by Jutamat Nacha, Hongyu Chen, Amorn Owatworakit, Kittirat Saharat, Anupong Makeudom and Sunita Chamyuang
Molecules 2025, 30(19), 3879; https://doi.org/10.3390/molecules30193879 - 25 Sep 2025
Abstract
Beta-glucans (β-glucans), polysaccharides found in cereals and fungi, are recognized for their prebiotic and potential anti-cancer activities, particularly in the colorectal area. This study aims to optimize the production of β-glucan through the solid-state fermentation of germinated Riceberry rice with Pleurotus ostreatus and [...] Read more.
Beta-glucans (β-glucans), polysaccharides found in cereals and fungi, are recognized for their prebiotic and potential anti-cancer activities, particularly in the colorectal area. This study aims to optimize the production of β-glucan through the solid-state fermentation of germinated Riceberry rice with Pleurotus ostreatus and evaluate the bioactivities of the resulting extract. The crude β-glucan extract, obtained with a recovery rate of 54.95% and 79.98% purity, demonstrated an effective extraction process, as confirmed by thermogravimetric analysis (TGA). Fourier-transform infrared spectroscopy (FTIR) analysis verified the presence of β-1,3/1,6-glycosidic linkages, characteristic of the bioactive β-glucans found in yeast and mushrooms. The biological assessment demonstrated the extract’s functional properties. At a concentration of 1 mg/mL, the crude β-glucan extract significantly promoted the growth of probiotics Lacticaseibacillus rhamnosus and Bacillus coagulans, exhibiting high Prebiotic Index (PI) values of 6.36 ± 0.72 and 115.70 ± 10.19, respectively, with PI values indicating strong prebiotic potential. For comparison, the standard prebiotic inulin yielded PI values of 0.41 ± 0.09 and 90.53 ± 2.28 for the same respective bacteria, highlighting the superior performance of the fungal-fermented β-glucan. Furthermore, the extract displayed efficacy in inhibiting colon cancer cells in preliminary in vitro tests. It reduced the viability of the SW480 colorectal cancer cell line by 66.23% and induced cell death in 27.94 ± 0.93% of the cells after 48 h of treatment, performing comparably to a commercial yeast β-glucan standard. Crucially, the extract showed no significant cytotoxicity toward the normal human colon cell line, CCD-841 CoN. These findings highlight the promising method of fungal solid-state fermentation on germinated Riceberry rice in the production of high-purity, bioactive β-glucans for use in functional foods. Full article
(This article belongs to the Special Issue New Development in Fermented Products—Third Edition)
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24 pages, 2611 KB  
Article
Enhancing the Cosmetic Potential of Aloe Vera Gel by Kombucha-Mediated Fermentation: Phytochemical Analysis and Evaluation of Antioxidant, Anti-Aging and Moisturizing Properties
by Aleksandra Ziemlewska, Martyna Zagórska-Dziok, Anna Nowak, Anna Muzykiewicz-Szymańska, Magdalena Wójciak, Ireneusz Sowa, Dariusz Szczepanek and Zofia Nizioł-Łukaszewska
Molecules 2025, 30(15), 3192; https://doi.org/10.3390/molecules30153192 - 30 Jul 2025
Cited by 2 | Viewed by 1369
Abstract
Aloe vera gel is a valuable raw material used in the cosmetic industry for its skin care properties. The present study analyzed the effects of the fermentation of aloe vera gel with a tea fungus kombucha, which is a symbiotic consortium of bacteria [...] Read more.
Aloe vera gel is a valuable raw material used in the cosmetic industry for its skin care properties. The present study analyzed the effects of the fermentation of aloe vera gel with a tea fungus kombucha, which is a symbiotic consortium of bacteria and yeast, carried out for 10 and 20 days (samples F10 and F20, respectively). The resulting ferments and unfermented gel were subjected to chromatographic analysis to determine the content of biologically active compounds. The permeability and accumulation of these compounds in pig skin were evaluated. In addition, the methods of DPPH, ABTS and the determination of intracellular free radical levels in keratinocytes (HaCaT) and fibroblasts (HDF) cell lines were used to determine antioxidant potential. The results showed a higher content of phenolic acids and flavonoids and better antioxidant properties of the ferments, especially after 20 days of fermentation. Cytotoxicity tests against HaCaT and HDF cells confirmed the absence of toxic effects; moreover, samples at the concentrations tested (mainly 10 and 25 mg/mL) showed cytoprotective effects. The analysis of enzymatic activity (collagenase, elastase and hyaluronidase) by the ELISA technique showed higher levels of inhibition for F10 and F20. The kombucha ferments also exhibited better moisturizing properties and lower levels of transepidermal water loss (TEWL), confirming their cosmetic potential. Full article
(This article belongs to the Special Issue New Development in Fermented Products—Third Edition)
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