Topical Collection "Technical Pitfalls and Biases in Molecular Biology"
Interests: nerve injury and neuropathic pain; pain and aging; central adaptations to chronic pain; multiple sclerosis; neuroinflammation; neuro-immunologic communication; redox signaling; nitric oxide; endocannabinoids and other lipid signaling molecules; progranulin; autophagy
Special Issues and Collections in MDPI journals
Special Issue in International Journal of Molecular Sciences: Molecular and Cellular Mechanisms of Pain
Special Issue in International Journal of Molecular Sciences: Neuronal Protein Homeostasis in Health and Disease
Special Issue in International Journal of Molecular Sciences: Pain and Inflammation
Special Issue in International Journal of Molecular Sciences: Mechanism of Longevity
Special Issue in International Journal of Molecular Sciences: Rhine-Main Neuroscience Network: rmn^2-Oberwesel 2018
Special Issue in International Journal of Molecular Sciences: Feature Annual Reviews in Molecular Sciences 2019
Topical Collection in International Journal of Molecular Sciences: Feature Papers in Molecular Biology
Special Issue in International Journal of Molecular Sciences: Molecular and Biological Mechanisms of Longevity
Special Issue in International Journal of Molecular Sciences: Molecular and Metabolic Mechanisms of Parkinson's Disease
The enormous progress of research techniques and tools and a steadily growing wealth of data increases the risk of putative technical errors, analytical biases, and erroneous analysis or mal-interpretation of research data. This Special Issue offers a platform to publish such errors and biases to increase the awareness and to improve the measures against such biases. For example, the off-target effects of gene manipulation or pharmacological interventions may produce unexpected effects, transgenic models may turn out to be something else, antibodies may un-specifically detect other proteins, alternative methods for normalization of omics data may yield controversial results, DNA/RNA extraction procedures may have a stronger effect on results than genotype or treatment, and differences between labs or day-to-day variability may be greater than the biological variability.
This Special Issue aims to gather a unique collection of original research articles and reviews, that address the occurrence, recognition, and avoidance of biases and errors and sharpen the awareness of experimental and data-analysis-based pitfalls. We welcome the submission of articles that cover, but are not limited to, the following topics:
- Analytical biases owing to sample handling or pre-analytical processing
- Impact of extraction procedures for DNA/RNA, proteins, metabolites, etc.
- Off-target effects of drugs or gene/protein manipulations
- Un-specificity of immune – or RNA-based detection and quantification methods
- Data-analysis based biases owing to normalization, data transformation, low expression genes/protein, low sample sizes, use of statistical methods, etc.
- Variability between labs or researchers versus biological variability
- Confounding influences of environmental factors
- Methods for visualizing biases
Prof. Dr. Irmgard Tegeder
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- analytical and technical biases
- molecular biology
- biological versus technical variability
- agreement of continuous variables
Jump to: 2019
Jump to: 2021
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Current projection methods-induced biases at subgroup detection for machine-learning based data-analysis of biomedical data
Authors: Jörn Lötsch and Alfred Ultsch