Special Issue "Heterologous Expression of Difficult to Produce Proteins in Bacterial Systems"
Deadline for manuscript submissions: closed (30 November 2019).
Interests: recombinant protein; protein aggregation; protein nanoparticles; nanobiotechnology; microbiology; inclusion bodies; animal production; antimicrobial proteins; immunomodulation
Interests: recombinant protein; protein engineering; nanobiotechnology; microbiology; protein nanoparticles; protein aggregation; functional amyloids; cell-targeted delivery
Many proteins of interest are produced in recombinant prokaryotic or eukaryotic expression systems. Among the prokaryotic expression systems, bacterial hosts are widely used for the production of recombinant proteins. Under overexpression conditions, the overproduced heterologous protein in recombinant bacteria can partition into two separate fractions: soluble and insoluble (also known as inclusion bodies). Often, the proteins of interest are difficult to express and, as a consequence, the final yields are unacceptable. In other cases, the proteins are prone to aggregation, making it challenging or impossible to obtain protein from the soluble fraction. In light of these issues, much of the research effort during the last few decades has gone towards the development of strategies to increase the efficiency of the production process for those difficult-to-obtain proteins. Thus, this Special Issue of IJMS will cover recent research activity towards the development of novel strategies used to obtain optimal yields of difficult-to-produce heterologous proteins which use bacterial expression systems as cell factories.
Dr. Elena Garcia-Fruitos
Dr. Neus Ferrer-Miralles
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
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- recombinant proteins
- heterologous proteins
- difficult-to-produce proteins
- bacterial expression systems
- strategies for protein production
- strategies for protein purification