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The Use of Molecular Biology in the Targeted Prevention, Diagnosis and Therapy of Microbial Infections

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 December 2025 | Viewed by 1030

Special Issue Editors


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Guest Editor
1. Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy
2. CIAS Research Center, University of Ferrara, 44122 Ferrara, Italy
Interests: bacteriology; virology; molecular biology; antimicrobial resistance; One Health approach; viral infections; human health

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Guest Editor
Department of Chemical, Pharmaceutical and Agricultural Sciences, Section of Microbiology, University of Ferrara, 44121 Ferrara, Italy
Interests: human herpesvirus 6 and human beta-herpesvirus infection; pathogenesis and disease association; autoimmune diseases
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Special Issue Information

Dear Colleagues,

Microbial infections are a constant and evolving threat to human health and welfare. The severity of infections is closely tied to the issue of antimicrobial resistance (AMR), which has been continuously increasing in the past decades, making it challenging to treat these infections.

It is widely acknowledged that the swift and precise identification of these pathogens is crucial for preventing, diagnosing, and treating the related diseases. Molecular approaches have become a valuable and efficient tool for microbiological diagnosis and surveillance. Specifically, the in-depth characterization of microbes and detailed pictures of their interaction with living beings can be obtained through approaches like next-generation technologies and PCR assays, overcoming the limitations of conventional microbiology.

In this Special Issue, we aim to explore the use of molecular biology in the targeted prevention, diagnosis and therapy of microbial infections. We are open to research that encompasses both human pathogens and animal husbandry and agriculture in a 'One Health' approach to tackling AMR.

To this purpose, we welcome both original and review papers that can help to further explore these issues, emphasizing the potential use of molecular approaches to identify microbes and understand their involvement in infections.

Dr. Maria D’Accolti
Dr. Elisabetta Caselli
Guest Editors

Manuscript Submission Information

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Keywords

  • microbial infections
  • AMR
  • molecular biology
  • One Health
  • microbiome

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Published Papers (1 paper)

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Review

26 pages, 955 KiB  
Review
Investigating Blood Biomarkers That Can Facilitate the Diagnosis of Meningitis—A Systematic Literature Review
by Jakub Marek Baran, Adrianna Porębska, Magdalena Lesisz, Katarzyna Polak, Olga Grodzka and Izabela Domitrz
Int. J. Mol. Sci. 2025, 26(4), 1427; https://doi.org/10.3390/ijms26041427 - 8 Feb 2025
Viewed by 705
Abstract
Meningitis is an inflammation of the meninges that can sometimes be a life-threatening disease. Therefore, fast and proper diagnosis with the implementation of adequate treatment is crucial in its management. Treatment depends on etiology, which can be viral, bacterial, fungal, and parasitic. Diagnosis [...] Read more.
Meningitis is an inflammation of the meninges that can sometimes be a life-threatening disease. Therefore, fast and proper diagnosis with the implementation of adequate treatment is crucial in its management. Treatment depends on etiology, which can be viral, bacterial, fungal, and parasitic. Diagnosis is based on thorough clinical examination with a performance of lumbar puncture in the case of meningitis suspicion. This procedure, however, remains invasive with several contraindications and a need for a patient’s consent, which is not always given due to the patient’s fear of it, for instance. Thus, this systematic review aimed to summarize the available literature on the topic of blood biomarkers in meningitis differentiation. A selection process was performed by two authors independently in accordance with the Preferred Research Items for Systematic Reviews and Meta-analyses. Two databases were screened. It led to the identification of 863 articles, of which 43 were eventually included in the systematic review. The analysis resulted in identifying blood biomarkers in both adult and pediatric meningitis. Most studies focused on inflammatory markers, such as C-reactive protein and procalcitonin, from which procalcitonin showed better utility. Among other analyzed molecules were, for instance, interleukins, apolipoproteins, and microRNAs. Moreover, many researchers suggested that combining biomarkers or implementing novel technologies may lead to the best accuracy. However, many suggested methods lack validation, which stands in the way of making them widely used. Full article
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