Plant Tissue and Organ Cultures for Crop Improvement in Omics Era: 2nd Edition

A special issue of Horticulturae (ISSN 2311-7524).

Deadline for manuscript submissions: 15 December 2025 | Viewed by 608

Special Issue Editor


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Guest Editor
1. Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 400084 Cluj-Napoca, Romania
2. Center of Systems Biology, Biodiversity and Bioresources, Babeş-Bolyai University, 400006 Cluj-Napoca, Romania
Interests: plant tissue culture; conservation of plant biodiversity; gene transfer
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Special Issue Information

Dear Colleagues,

Following the success of the first edition of this Special Issue, “Plant Tissue and Organ Cultures for Crop Improvement in Omics Era”, a second edition is being launched.

Plant tissue and organ cultures have been employed over the years for plant regeneration, multiplication, germplasm conservation, production of secondary metabolites, and improvement of genetic characteristics by somaclonal and gametoclonal variations. In the last few decades, plant tissue cultures have also been used as experimental systems for genetic transformation and genome editing of several plant species for the development of smart crops with improved quality and yield. Thus, the plant tissue and organ culture constitute one of the most promising tools in research and biotechnology. Therefore, this Special Issue of ‘Plant Tissue and Organ Cultures for Crop Improvement in Omics Era: 2nd Edition’ will be focused on the most recent studies on the following topics (but not exclusively): gene expression during organogenesis and somatic embryogenesis, transcription and translation studies in gene regulation, genetic and epigenetic changes associated with in vitro culture conditions, gene transfer, genome editing, metabolic engineering, and molecular breeding.

Dr. Anca Butiuc-Keul
Guest Editor

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Keywords

  • bioactive compounds
  • gene regulation
  • gene transfer
  • genome editing
  • horticultural crops
  • micropropagation
  • organogenesis
  • plant tissue culture
  • plant transformation

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Published Papers (1 paper)

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Research

12 pages, 2175 KiB  
Article
UPLC-QTOF-ESI-MS/MS-Based Comparative Study of Phytochemicals in Sapindus mukorossi
by Neil Patrick Uy, Hak-Dong Lee, Jajung Ku, Kyung Choi and Sanghyun Lee
Horticulturae 2025, 11(6), 682; https://doi.org/10.3390/horticulturae11060682 - 13 Jun 2025
Viewed by 468
Abstract
This study examined the phytochemical compositions of ten Sapindus mukorossi samples from two regions in Korea: Suwon and Daegu. The Folin–Ciocalteu method was used to calculate the total polyphenol content (TPC). Among all extracts tested, leaf samples from Suwon and Daegu (SLE and [...] Read more.
This study examined the phytochemical compositions of ten Sapindus mukorossi samples from two regions in Korea: Suwon and Daegu. The Folin–Ciocalteu method was used to calculate the total polyphenol content (TPC). Among all extracts tested, leaf samples from Suwon and Daegu (SLE and DLE) exhibited the highest TPC at 2.70 and 2.90 mg tannic acid equivalent/g extract. Similarly, a modified aluminum chloride colorimetric test was used to determine the total flavonoid content (TFC). Similar results were obtained, with SLE and DLE having TFC values of 40.71 and 41.07 mg quercetin equivalent/g extract, respectively. Liquid chromatography with tandem mass spectrometry was used to detect 13 compounds, whereas high-performance liquid chromatography was used to quantify the prominent compounds: rutin, nicotiflorin, and narcissin. Among these, rutin was the most abundant, especially in SLE and DLE (54.37 and 70.21 mg/g, respectively). Furthermore, rutin significantly contributed to the total content of these samples at 78.31 and 85.44 mg/g, respectively. There were significant variations in the distribution of these compounds across different parts of the plant. These findings highlight the importance of S. mukorossi as a source of natural bioactive chemicals and pave the way for further research into its potential applications in healthcare products. Full article
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